################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AhdI Alw44I AlwNI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspHI BsrGI Bst1107I BstAPI BstBI BstYI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MflI MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 118 CGCG CG^CG AccIII 739 TCCGGA T^CCGGA AcsI 715 AAATTT [GA]^AATT[CT] AgeI 707 ACCGGT A^CCGGT AlwI 867 GGATC GGATC(4/5) AocI 67 CCTGAGG CC^T[ACGT]AGG Aor51HI 400 AGCGCT AGC^GCT ApoI 715 AAATTT [GA]^AATT[CT] AspHI 946 GAGCTC G[AT]GC[AT]^C AspI 144 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 502 CCTAGG C^CTAGG BanI 1021 GGCACC G^G[CT][GA]CC BlnI 502 CCTAGG C^CTAGG BlpI 1065 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1065 GCTCAGC GC^T[ACGT]AGC BseAI 739 TCCGGA T^CCGGA BsgI 97 GTGCAG GTGCAG(16/14) BsiHKAI 946 GAGCTC G[AT]GC[AT]^C BsmI 1014 GAATGC GAATGC(1/-1) BspEI 739 TCCGGA T^CCGGA BsrBI 547 GAGCGG CCGCTC(-3/-3) BsrDI 929 CATTGC GCAATG(2/0) BssHII 117 GCGCGC G^CGCGC BstEII 45 GGTGACC G^GT[ACGT]ACC BstPI 45 GGTGACC G^GT[ACGT]ACC BstUI 118 CGCG CG^CG Bsu36I 67 CCTGAGG CC^T[ACGT]AGG CelII 1065 GCTCAGC GC^T[ACGT]AGC DpnII 868 GATC ^GATC DraII 764 AGGCCCT [GA]G^G[ACGT]CC[CT] Eco47III 400 AGCGCT AGC^GCT Eco81I 67 CCTGAGG CC^T[ACGT]AGG EcoO109I 764 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 45 GGTGACC G^GT[ACGT]ACC HincII 1074 GTTGAC GT[CT]^[GA]AC HindII 1074 GTTGAC GT[CT]^[GA]AC MaeII 1049 ACGT A^CGT MboI 868 GATC ^GATC MroI 739 TCCGGA T^CCGGA MseI 248 TTAA T^TAA MspA1I 238 CAGCTG C[AC]G^C[GT]G MvnI 118 CGCG CG^CG NcoI 914 CCATGG C^CATGG NdeII 868 GATC ^GATC NheI 787 GCTAGC G^CTAGC PflFI 144 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 707 ACCGGT A^CCGGT PshAI 48 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 238 CAGCTG CAG^CTG SacI 946 GAGCTC GAGCT^C SapI 948 GCTCTTC GCTCTTC(1/4) Sau3AI 868 GATC ^GATC ScaI 749 AGTACT AGT^ACT TfiI 1135 GATTC G^A[AT]TC Tru9I 248 TTAA T^TAA Tth111I 144 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflIII 74 ACATGT A^C[GA][CT]GT 659 ACATGT AvaI 58 CCCGAG C^[CT]CG[GA]G 476 CTCGGG BalI 335 TGGCCA TGG^CCA 620 TGGCCA BanII 313 GAGCCC G[GA]GC[CT]^C 946 GAGCTC BbsI 678 GAAGAC GAAGAC(2/6) 1043 GTCTTC BcnI 177 CCGGG CC^[GC]GG 537 CCGGG BmyI 313 GAGCCC G[AGT]GC[ACT]^C 946 GAGCTC BpuAI 678 GAAGAC GAAGAC(2/6) 1043 GTCTTC BseRI 735 CTCCTC GAGGAG(10/8) 61 GAGGAG BsmFI 253 GGGAC GGGAC(10/14) 55 GTCCC BsoBI 58 CCCGAG C^[CT]CG[GA]G 476 CTCGGG Bsp1286I 313 GAGCCC G[AGT]GC[ACT]^C 946 GAGCTC BspLU11I 74 ACATGT A^CATGT 659 ACATGT BspMI 99 GCAGGT ACCTGC(4/8) 891 GCAGGT BsrFI 707 ACCGGT [GA]^CCGG[CT] 980 GCCGGT BssSI 7 CTCGTG CACGAG(-5/-1) 94 CTCGTG BstXI 302 CCATCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1071 CCAGTTGACTGG Cfr10I 707 ACCGGT [GA]^CCGG[CT] 980 GCCGGT DsaI 155 CCACGG C^C[GA][CT]GG 914 CCATGG EaeI 335 TGGCCA [CT]^GGCC[GA] 620 TGGCCA EarI 949 CTCTTC CTCTTC(1/4) 1147 GAAGAG EcoNI 93 CCTCGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 756 CCTGAAAGAGG FauI 37 GCGGG CCCGC(4/6) 243 GCGGG HaeII 400 AGCGCT [GA]GCGC^[CT] 581 AGCGCC Ksp632I 949 CTCTTC CTCTTC(1/4) 1147 GAAGAG MfeI 519 CAATTG C^AATTG 920 CAATTG MluNI 335 TGGCCA TGG^CCA 620 TGGCCA MscI 335 TGGCCA TGG^CCA 620 TGGCCA MunI 519 CAATTG C^AATTG 920 CAATTG NciI 177 CCGGG CC^[GC]GG 537 CCGGG SmlI 227 CTCAAG C^T[CT][GA]AG 886 CTCAAG StuI 380 AGGCCT AGG^CCT 432 AGGCCT XcmI 155 CCACGGGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 905 CCAATGAAGCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BfaI BpmI BsaJI BsaWI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboII MnlI MslI MspI MvaI MwoI NlaIII NlaIV NspI PleI RsaI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I