(Circular) (Six-base) MAPSORT of: pFN21AE2612.seq Check: 7994 from: 1 to: 5860 pFN21AE2612 5860 bp With 182 enzymes: SgfI * March 10, 2016 11:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2095 2887 2095 Size: 792 5068 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3203 1937 Size: 1266 4594 Acc65I G'GTAC_C Cuts at: 3181 3181 Size: 5860 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2711 2941 3171 3923 5307 5575 1632 Size: 1079 230 230 752 1384 268 1917 Fragments arranged by size: 1917 1384 1079 752 268 230 230 AfeI AGC'GCT Cuts at: 2407 2407 Size: 5860 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 2079 2664 4759 5736 1203 Size: 876 585 2095 977 1327 Fragments arranged by size: 2095 1327 977 876 585 AgeI A'CCGG_T Cuts at: 2712 4691 2712 Size: 1979 3881 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5860 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2556 2588 2556 Size: 32 5828 AlwNI CAG_nnn'CTG Cuts at: 3603 5175 3603 Size: 1572 4288 ApaLI G'TGCA_C Cuts at: 3917 5073 3917 Size: 1156 4704 ApoI r'AATT_y Cuts at: 1493 2720 3169 3395 3547 5656 1493 Size: 1227 449 226 152 2109 1697 Fragments arranged by size: 2109 1697 1227 449 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2481 3185 1870 Size: 73 120 418 704 4545 Fragments arranged by size: 4545 704 418 120 73 AvrII C'CTAG_G Cuts at: 2507 2507 Size: 5860 BamHI G'GATC_C Cuts at: 3190 3557 3190 Size: 367 5493 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3026 3181 4636 618 Size: 325 217 627 1239 155 1455 1842 Fragments arranged by size: 1842 1455 1239 627 325 217 155 BanII G_rGCy'C Cuts at: 1391 2322 2955 3179 1391 Size: 931 633 224 4072 Fragments arranged by size: 4072 931 633 224 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2690 3041 928 Size: 971 791 351 3747 Fragments arranged by size: 3747 971 791 351 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4051 4085 1903 Size: 34 2114 34 3678 Fragments arranged by size: 3678 2114 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 1548 Size: 5860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4471 136 Size: 107 122 71 631 605 2799 1525 Fragments arranged by size: 2799 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5855 5855 Size: 5860 BlpI GC'TnA_GC Cuts at: 3071 3277 3071 Size: 206 5654 Bme1580I G_kGCm'C Cuts at: 1790 3921 5077 1790 Size: 2131 1156 2573 Fragments arranged by size: 2573 2131 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3093 3591 4549 Size: 416 53 83 399 24 1676 498 958 Cuts at: 4549 442 Size: 1753 Fragments arranged by size: 1753 1676 958 498 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2796 1056 Size: 1740 4120 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2390 2422 4520 1239 Size: 1097 54 32 2098 2579 Fragments arranged by size: 2579 2098 1097 54 32 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2217 2876 3985 4850 5148 2217 Size: 659 1109 865 298 2929 Fragments arranged by size: 2929 1109 865 659 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4523 882 Size: 617 3024 2219 Fragments arranged by size: 3024 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3651 493 Size: 711 2447 2702 Fragments arranged by size: 2702 2447 711 BsaBI GATnn'nnATC Cuts at: 5854 5854 Size: 5860 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3667 4049 4684 275 Size: 53 83 186 936 2134 382 635 1451 Fragments arranged by size: 2134 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2712 2744 2869 4293 4691 4965 5112 5523 2712 Size: 32 125 1424 398 274 147 411 3049 Fragments arranged by size: 3049 1424 411 398 274 147 125 32 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2741 2771 1178 Size: 30 165 30 1338 30 4267 Fragments arranged by size: 4267 1338 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2731 1186 Size: 895 650 4315 Fragments arranged by size: 4315 895 650 BseYI C'CCAG_C Cuts at: 2395 2465 2819 5063 5559 2395 Size: 70 354 2244 496 2696 Fragments arranged by size: 2696 2244 496 354 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 1163 Size: 573 387 4900 Fragments arranged by size: 4900 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 4071 5099 1540 Size: 275 186 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2955 3921 4006 5077 2955 Size: 966 85 1071 3738 Fragments arranged by size: 3738 1071 966 85 BsmI GAATG_Cn' Cuts at: 3025 3371 3464 3025 Size: 346 93 5421 Fragments arranged by size: 5421 346 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2322 2955 3179 3921 4006 5077 1391 Size: 399 532 633 224 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 633 532 399 224 85 BspEI T'CCGG_A Cuts at: 2744 5523 2744 Size: 2779 3081 BspHI T'CATG_A Cuts at: 3749 5479 3749 Size: 1730 4130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2095 2887 3215 844 Size: 1251 792 328 3489 Fragments arranged by size: 3489 1251 792 328 BsrBI CCG'CTC Cuts at: 1394 1823 2554 3747 1394 Size: 429 731 1193 3507 Fragments arranged by size: 3507 1193 731 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2933 4355 4529 65 Size: 1183 1685 1422 174 1396 Fragments arranged by size: 1685 1422 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2712 2985 4504 4691 1700 Size: 110 902 273 1519 187 2869 Fragments arranged by size: 2869 1519 902 273 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 2122 1925 Size: 197 5663 BssSI C'ACGA_G Cuts at: 2012 2099 3920 4932 2012 Size: 87 1821 1012 2940 Fragments arranged by size: 2940 1821 1012 87 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5860 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 2050 1184 Size: 866 4994 BstXI CCAn_nnnn'nTGG Cuts at: 2314 3083 2314 Size: 769 5091 BstYI r'GATC_y Cuts at: 1972 3190 3557 3942 3959 5411 5497 5509 5855 Size: 1218 367 385 17 1452 86 12 346 Cuts at: 5855 1972 Size: 1977 Fragments arranged by size: 1977 1452 1218 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2073 2073 Size: 5860 BtgI C'CryG_G Cuts at: 513 1065 2160 2919 513 Size: 552 1095 759 3454 Fragments arranged by size: 3454 1095 759 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2961 487 Size: 683 56 1735 3386 Fragments arranged by size: 3386 1735 683 56 BtsI GCAGTG_nn' Cuts at: 2357 2685 3376 4170 4190 2357 Size: 328 691 794 20 4027 Fragments arranged by size: 4027 794 691 328 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2340 2625 3307 Size: 54 1425 50 275 18 510 285 682 Cuts at: 3307 4196 5825 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 682 510 285 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2960 3147 3790 1353 Size: 1607 187 643 3423 Fragments arranged by size: 3423 1607 643 187 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4447 4821 4967 387 Size: 1064 138 2858 374 146 1280 Fragments arranged by size: 2858 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2953 2953 Size: 5860 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2390 2422 2711 2941 3171 3923 Size: 393 704 54 32 289 230 230 752 Cuts at: 3923 4520 5307 5575 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 752 704 597 393 289 268 230 230 54 32 EcoNI CCTnn'n_nnAGG Cuts at: 2102 2765 2102 Size: 663 5197 EcoO109I rG'GnC_Cy Cuts at: 2770 2770 Size: 5860 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2789 2789 Size: 5860 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 2409 2590 5007 1678 Size: 731 181 2417 2531 Fragments arranged by size: 2531 2417 731 181 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2213 2245 2464 2496 4501 Size: 32 135 32 641 32 219 32 2005 Cuts at: 4501 4533 4575 4607 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2005 641 219 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3081 3204 3450 669 Size: 988 281 1143 123 246 3079 Fragments arranged by size: 3079 1143 988 281 246 123 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KpnI G_GTAC'C Cuts at: 3185 3185 Size: 5860 MfeI C'AATT_G Cuts at: 2524 2925 3459 2524 Size: 401 534 4925 Fragments arranged by size: 4925 534 401 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4974 5158 1305 Size: 3669 184 2007 Fragments arranged by size: 3669 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2342 2627 10 Size: 54 1425 343 510 285 3243 Fragments arranged by size: 3243 1425 510 343 285 54 MslI CAynn'nnrTG Cuts at: 518 1166 2601 2621 2967 3818 4177 4336 518 Size: 648 1435 20 346 851 359 159 2042 Fragments arranged by size: 2042 1435 851 648 359 346 159 20 MspA1I CmG'CkG Cuts at: 1606 2245 3276 3953 5101 5346 1606 Size: 639 1031 677 1148 245 2120 Fragments arranged by size: 2120 1148 1031 677 639 245 NcoI C'CATG_G Cuts at: 513 1065 2919 513 Size: 552 1854 3454 Fragments arranged by size: 3454 1854 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NheI G'CTAG_C Cuts at: 1052 2792 1052 Size: 1740 4120 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 2083 2668 2738 3218 4763 1132 Size: 97 854 585 70 480 1545 2229 Fragments arranged by size: 2229 1545 854 585 480 97 70 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 2079 2664 4759 2079 Size: 585 2095 3180 Fragments arranged by size: 3180 2095 585 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5860 PfoI T'CCnGG_A Cuts at: 2703 2703 Size: 5860 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2057 1613 Size: 444 5416 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PstI C_TGCA'G Cuts at: 830 3212 830 Size: 2382 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2245 2245 Size: 5860 SacI G_AGCT'C Cuts at: 2955 2955 Size: 5860 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SapI GCTCTTCn'nnn_ Cuts at: 2960 2960 Size: 5860 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 2756 4108 5820 2756 Size: 1352 1712 2796 Fragments arranged by size: 2796 1712 1352 SfcI C'TryA_G Cuts at: 826 1045 3208 4343 5024 5215 826 Size: 219 2163 1135 681 191 1471 Fragments arranged by size: 2163 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 3187 1872 Size: 1315 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2232 2891 3964 4865 5127 820 Size: 197 926 289 659 1073 901 262 1553 Fragments arranged by size: 1553 1073 926 901 659 289 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 2387 2439 1517 Size: 870 52 4938 Fragments arranged by size: 4938 870 52 StyI C'CwwG_G Cuts at: 513 1065 2487 2507 2547 2835 2919 3299 513 Size: 552 1422 20 40 288 84 380 3074 Fragments arranged by size: 3074 1422 552 380 288 84 40 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2702 3887 3904 4057 4242 4687 325 Size: 1255 1122 1185 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1185 1122 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2754 4106 5818 Size: 274 80 33 51 1443 777 1352 1712 Cuts at: 5818 96 Size: 138 Fragments arranged by size: 1712 1443 1352 777 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2741 2773 5820 5852 2741 Size: 32 3047 32 2749 Fragments arranged by size: 3047 2749 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2152 1316 Size: 836 5024 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 2917 2167 Size: 750 5110 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 3185 1870 Size: 1315 4545 XmnI GAAnn'nnTTC Cuts at: 3989 3989 Size: 5860 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI