################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BfrI BglI BlnI BsaBI BsaHI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MluI MroI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI StuI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 1489 CGCG CG^CG AflIII 65 ACGTGT A^C[GA][CT]GT ApaI 1621 GGGCCC GGGCC^C BalI 760 TGGCCA TGG^CCA BanI 2064 GGCACC G^G[CT][GA]CC BanII 1621 GGGCCC G[GA]GC[CT]^C BciVI 1956 GGATAC GTATCC(6/5) BcnI 1829 CCCGG CC^[GC]GG BglII 1762 AGATCT A^GATCT BlpI 531 GCTAAGC GC^T[ACGT]AGC Bpu1102I 531 GCTAAGC GC^T[ACGT]AGC BsaWI 1634 TCCGGT [AT]^CCGG[AT] BsmBI 1479 GAGACG CGTCTC(1/5) BsmI 1928 GCATTC GAATGC(1/-1) BsrBI 1604 GAGCGG CCGCTC(-3/-3) BsrDI 813 CATTGC GCAATG(2/0) BsrFI 80 GCCGGC [GA]^CCGG[CT] BssSI 1332 CACGAG CACGAG(-5/-1) BstUI 1489 CGCG CG^CG CelII 531 GCTAAGC GC^T[ACGT]AGC Cfr10I 80 GCCGGC [GA]^CCGG[CT] DsaI 757 CCATGG C^C[GA][CT]GG EaeI 760 TGGCCA [CT]^GGCC[GA] HgaI 1481 GACGC GACGC(5/10) HincII 403 GTTGAC GT[CT]^[GA]AC HindII 403 GTTGAC GT[CT]^[GA]AC MfeI 347 CAATTG C^AATTG MluNI 760 TGGCCA TGG^CCA MscI 760 TGGCCA TGG^CCA MspA1I 1019 CAGCTG C[AC]G^C[GT]G MunI 347 CAATTG C^AATTG MvnI 1489 CGCG CG^CG NaeI 80 GCCGGC GCC^GGC NciI 1829 CCCGG CC^[GC]GG NcoI 757 CCATGG C^CATGG NgoMIV 80 GCCGGC G^CCGGC PflMI 286 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 1019 CAGCTG CAG^CTG SbfI 2250 CCTGCAGG CCTGCA^GG ScaI 1908 AGTACT AGT^ACT SmlI 2081 CTCAAG C^T[CT][GA]AG Sse8387I 2250 CCTGCAGG CCTGCA^GG Van91I 286 CCAGCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 1943 GAATTT [GA]^AATT[CT] 2087 AAATTC AlwNI 1834 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 2292 CAGTGCCTG ApoI 1943 GAATTT [GA]^AATT[CT] 2087 AAATTC AspI 1661 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1842 GACCCAGTC AvaI 1238 CTCGGG C^[CT]CG[GA]G 2032 CCCGAG BclI 497 TGATCA T^GATCA 1062 TGATCA BfaI 1357 CTAG C^TAG 1933 CTAG BsaAI 355 TACGTG [CT]AC^GT[GA] 1114 TACGTG BsaI 780 GAGACC GGTCTC(1/5) 1990 GAGACC BseRI 1433 GAGGAG GAGGAG(10/8) 1615 GAGGAG BsgI 653 CTGCAC GTGCAG(16/14) 517 GTGCAG BsoBI 1238 CTCGGG C^[CT]CG[GA]G 2032 CCCGAG Bsp1407I 159 TGTACA T^GTACA 1521 TGTACA BsrGI 159 TGTACA T^GTACA 1521 TGTACA BstYI 1359 AGATCC [GA]^GATC[CT] 1762 AGATCT EarI 1147 CTCTTC CTCTTC(1/4) 777 GAAGAG EcoNI 886 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2055 CCTTCTGCAGG FbaI 497 TGATCA T^GATCA 1062 TGATCA HphI 309 GGTGA GGTGA(8/7) 1987 GGTGA Ksp632I 1147 CTCTTC CTCTTC(1/4) 777 GAAGAG MaeI 1357 CTAG C^TAG 1933 CTAG MflI 1359 AGATCC [GA]^GATC[CT] 1762 AGATCT NspI 409 ACATGC [GA]CATG^[CT] 2027 ACATGC PflFI 1661 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1842 GACCCAGTC SspBI 159 TGTACA T^GTACA 1521 TGTACA Tth111I 1661 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1842 GACCCAGTC XhoII 1359 AGATCC [GA]^GATC[CT] 1762 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BbsI BbvI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I