(Circular) (Six-base) MAPSORT of: pFN21AE6705.seq Check: 7990 from: 1 to: 7006 pFN21AE6705 7006 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4249 4249 Size: 7006 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4816 278 Size: 53 83 186 936 3280 2468 Fragments arranged by size: 3280 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4349 1937 Size: 2412 4594 Acc65I G'GTAC_C Cuts at: 4327 4327 Size: 7006 AclI AA'CG_TT Cuts at: 1563 5133 5506 1563 Size: 3570 373 3063 Fragments arranged by size: 3570 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3272 5069 6453 6721 1632 Size: 1640 1797 1384 268 1917 Fragments arranged by size: 1917 1797 1640 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6809 AflIII A'CryG_T Cuts at: 1203 2070 5905 6882 1203 Size: 867 3835 977 1327 Fragments arranged by size: 3835 1327 977 867 AgeI A'CCGG_T Cuts at: 5837 5837 Size: 7006 AhdI GACnn_n'nnGTC Cuts at: 5735 5735 Size: 7006 AleI CACnn'nnGTG Cuts at: 1166 2804 1166 Size: 1638 5368 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2697 2714 1184 Size: 1513 17 5476 Fragments arranged by size: 5476 1513 17 AlwNI CAG_nnn'CTG Cuts at: 3844 4302 4749 6321 3844 Size: 458 447 1572 4529 Fragments arranged by size: 4529 1572 458 447 ApaI G_GGCC'C Cuts at: 3630 3630 Size: 7006 ApaLI G'TGCA_C Cuts at: 5063 6219 5063 Size: 1156 5850 ApoI r'AATT_y Cuts at: 1493 3948 4092 4315 4541 4693 6802 1493 Size: 2455 144 223 226 152 2109 1697 Fragments arranged by size: 2455 2109 1697 226 223 152 144 AseI AT'TA_AT Cuts at: 160 1032 5560 160 Size: 872 4528 1606 Fragments arranged by size: 4528 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7006 AvaI C'yCGr_G Cuts at: 1870 1943 3243 4037 4331 1870 Size: 73 1300 794 294 4545 Fragments arranged by size: 4545 1300 794 294 73 BamHI G'GATC_C Cuts at: 4336 4703 4336 Size: 367 6639 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4069 4327 5782 618 Size: 325 217 627 2282 258 1455 1842 Fragments arranged by size: 2282 1842 1455 627 325 258 217 BanII G_rGCy'C Cuts at: 1391 3630 4325 1391 Size: 2239 695 4072 Fragments arranged by size: 4072 2239 695 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2465 2560 3705 928 Size: 971 566 95 1145 4229 Fragments arranged by size: 4229 1145 971 566 95 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5197 5231 1903 Size: 34 3260 34 3678 Fragments arranged by size: 3678 3260 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3955 4898 6108 3955 Size: 943 1210 4853 Fragments arranged by size: 4853 1210 943 BclI T'GATC_A Cuts at: 1548 2502 3067 1548 Size: 954 565 5487 Fragments arranged by size: 5487 954 565 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5617 136 Size: 107 122 71 631 605 3945 1525 Fragments arranged by size: 3945 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3767 7001 3767 Size: 3234 3772 BlpI GC'TnA_GC Cuts at: 2537 4423 2537 Size: 1886 5120 Bme1580I G_kGCm'C Cuts at: 1790 2843 3064 3630 5067 6223 1790 Size: 1053 221 566 1437 1156 2573 Fragments arranged by size: 2573 1437 1156 1053 566 221 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7006 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2335 3844 4000 Size: 416 53 83 399 24 918 1509 156 Cuts at: 4000 4737 5695 442 Size: 737 958 1753 Fragments arranged by size: 1753 1509 958 918 737 416 399 156 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7006 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3786 3786 Size: 7006 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2111 2274 2441 2466 2855 2901 3132 Size: 800 72 163 167 25 389 46 231 Cuts at: 3132 3457 4245 4279 5666 1239 Size: 325 788 34 1387 2579 Fragments arranged by size: 2579 1387 800 788 389 325 231 167 163 72 46 34 25 Bpu10I CC'TnA_GC Cuts at: 3539 3539 Size: 7006 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4071 5131 5996 6294 4071 Size: 1060 865 298 4783 Fragments arranged by size: 4783 1060 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2779 3989 5669 882 Size: 617 1280 1210 1680 2219 Fragments arranged by size: 2219 1680 1280 1210 617 BsaAI yAC'GTr Cuts at: 493 1204 2362 3121 4797 493 Size: 711 1158 759 1676 2702 Fragments arranged by size: 2702 1676 1158 759 711 BsaBI GATnn'nnATC Cuts at: 7000 7000 Size: 7006 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4813 5195 5830 275 Size: 53 83 186 936 3280 382 635 1451 Fragments arranged by size: 3280 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3639 5439 5837 6111 6258 6669 3639 Size: 1800 398 274 147 411 3976 Fragments arranged by size: 3976 1800 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3077 3105 3107 3135 3872 Size: 30 165 30 1674 28 2 28 737 Cuts at: 3872 3902 1178 Size: 30 4282 Fragments arranged by size: 4282 1674 737 165 30 30 30 28 28 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3453 3635 1186 Size: 2267 182 4557 Fragments arranged by size: 4557 2267 182 BseYI C'CCAG_C Cuts at: 6209 6705 6209 Size: 496 6510 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2543 2643 1163 Size: 573 807 100 5526 Fragments arranged by size: 5526 807 573 100 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4456 5217 6245 1540 Size: 275 186 2455 761 1028 2301 Fragments arranged by size: 2455 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2380 2676 3639 4231 5067 5152 6223 2380 Size: 296 963 592 836 85 1071 3163 Fragments arranged by size: 3163 1071 963 836 592 296 85 BsmI GAATG_Cn' Cuts at: 3933 4517 4610 3933 Size: 584 93 6329 Fragments arranged by size: 6329 584 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3478 3478 Size: 7006 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2380 2676 2843 3064 3630 3639 4231 Size: 399 590 296 167 221 566 9 592 Cuts at: 4231 4325 5067 5152 6223 1391 Size: 94 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 592 590 566 399 296 221 167 94 85 9 BspEI T'CCGG_A Cuts at: 6669 6669 Size: 7006 BspHI T'CATG_A Cuts at: 2530 2979 3861 4895 6625 2530 Size: 449 882 1034 1730 2911 Fragments arranged by size: 2911 1730 1034 882 449 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2332 2515 2665 4249 4361 844 Size: 1488 183 150 1584 112 3489 Fragments arranged by size: 3489 1584 1488 183 150 112 BsrBI CCG'CTC Cuts at: 1394 1823 3611 4893 1394 Size: 429 1788 1282 3507 Fragments arranged by size: 3507 1788 1282 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2817 5501 5675 65 Size: 1183 1569 2684 174 1396 Fragments arranged by size: 2684 1569 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 5650 5837 1700 Size: 110 275 3565 187 2869 Fragments arranged by size: 3565 2869 275 187 110 BsrGI T'GTAC_A Cuts at: 96 2164 3526 96 Size: 2068 1362 3576 Fragments arranged by size: 3576 2068 1362 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7006 BssSI C'ACGA_G Cuts at: 3337 5066 6078 3337 Size: 1729 1012 4265 Fragments arranged by size: 4265 1729 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2657 3218 4063 794 Size: 1863 561 845 3737 Fragments arranged by size: 3737 1863 845 561 BstBI TT'CG_AA Cuts at: 5810 5810 Size: 7006 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7006 BstXI CCAn_nnnn'nTGG Cuts at: 2089 2806 2903 2089 Size: 717 97 6192 Fragments arranged by size: 6192 717 97 BstYI r'GATC_y Cuts at: 1972 3364 3767 4336 4703 5088 5105 6557 6643 Size: 1392 403 569 367 385 17 1452 86 Cuts at: 6643 6655 7001 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1392 569 403 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2762 513 Size: 552 939 758 4757 Fragments arranged by size: 4757 939 758 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3490 487 Size: 683 56 2264 4003 Fragments arranged by size: 4003 2264 683 56 BtsI GCAGTG_nn' Cuts at: 3037 3585 4522 5316 5336 3037 Size: 548 937 794 20 4707 Fragments arranged by size: 4707 937 794 548 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7006 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6971 DraI TTT'AAA Cuts at: 4310 4310 Size: 7006 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6013 809 Size: 507 4697 1802 Fragments arranged by size: 4697 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2765 4453 5342 Size: 54 1425 50 275 18 935 1688 889 Cuts at: 5342 6971 8 Size: 1629 43 Fragments arranged by size: 1688 1629 1425 935 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4453 1537 Size: 275 2641 4090 Fragments arranged by size: 4090 2641 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2777 3158 4936 1353 Size: 1424 381 1778 3423 Fragments arranged by size: 3423 1778 1424 381 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5593 5967 6113 387 Size: 1064 138 4004 374 146 1280 Fragments arranged by size: 4004 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2111 2274 2441 2466 2855 2901 Size: 393 407 72 163 167 25 389 46 Cuts at: 2901 3132 3272 3457 4245 4279 5069 5666 6453 Size: 231 140 185 788 34 790 597 787 Cuts at: 6453 6721 1239 Size: 268 1524 Fragments arranged by size: 1524 790 788 787 597 407 393 389 268 231 185 167 163 140 72 46 34 25 EcoNI CCTnn'n_nnAGG Cuts at: 2895 4064 2895 Size: 1169 5837 EcoO109I rG'GnC_Cy Cuts at: 2758 2885 2900 3626 3841 2758 Size: 127 15 726 215 5923 Fragments arranged by size: 5923 726 215 127 15 EcoRI G'AATT_C Cuts at: 4315 4693 4315 Size: 378 6628 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2450 2450 Size: 7006 FspI TGC'GCA Cuts at: 5512 5512 Size: 7006 HaeII r_GCGC'y Cuts at: 1678 6153 1678 Size: 4475 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2850 2882 3356 3388 3429 Size: 32 135 32 1278 32 474 32 41 Cuts at: 3429 3461 3645 3677 3794 3826 4218 4250 5647 Size: 32 184 32 117 32 392 32 1397 Cuts at: 5647 5679 5721 5753 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1397 1278 474 392 184 135 117 42 41 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2410 4350 4596 669 Size: 988 281 472 1940 246 3079 Fragments arranged by size: 3079 1940 988 472 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7006 HpaI GTT'AAC Cuts at: 4596 4596 Size: 7006 KpnI G_GTAC'C Cuts at: 4331 4331 Size: 7006 MfeI C'AATT_G Cuts at: 2352 4605 2352 Size: 2253 4753 MluI A'CGCG_T Cuts at: 6882 6882 Size: 7006 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2045 2896 3688 6120 6304 1305 Size: 740 851 792 2432 184 2007 Fragments arranged by size: 2432 2007 851 792 740 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2767 10 Size: 54 1425 343 935 4249 Fragments arranged by size: 4249 1425 935 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2639 2804 3494 4964 5323 5482 518 Size: 648 1473 165 690 1470 359 159 2042 Fragments arranged by size: 2042 1473 1470 690 648 359 165 159 MspA1I CmG'CkG Cuts at: 1606 3026 4422 5099 6247 6492 1606 Size: 1420 1396 677 1148 245 2120 Fragments arranged by size: 2120 1420 1396 1148 677 245 NaeI GCC'GGC Cuts at: 2087 2087 Size: 7006 NcoI C'CATG_G Cuts at: 513 1065 2004 2762 513 Size: 552 939 758 4757 Fragments arranged by size: 4757 939 758 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7006 NgoMIV G'CCGG_C Cuts at: 2085 2085 Size: 7006 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7006 NotI GC'GGCC_GC Cuts at: 4453 4453 Size: 7006 NspI r_CATG'y Cuts at: 1132 1229 2418 4036 4364 5909 1132 Size: 97 1189 1618 328 1545 2229 Fragments arranged by size: 2229 1618 1545 1189 328 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7006 PciI A'CATG_T Cuts at: 5905 5905 Size: 7006 PflMI CCAn_nnn'nTGG Cuts at: 1108 2297 1108 Size: 1189 5817 PmeI GTTT'AAAC Cuts at: 4310 4310 Size: 7006 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5075 5107 6588 6620 5075 Size: 32 1481 32 5461 Fragments arranged by size: 5461 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2758 2885 3841 2758 Size: 127 956 5923 Fragments arranged by size: 5923 956 127 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7006 PsiI TTA'TAA Cuts at: 4576 4576 Size: 7006 PspOMI G'GGCC_C Cuts at: 3626 3626 Size: 7006 PstI C_TGCA'G Cuts at: 830 2460 2703 4068 4260 4358 830 Size: 1630 243 1365 192 98 3478 Fragments arranged by size: 3478 1630 1365 243 192 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7006 PvuII CAG'CTG Cuts at: 3026 3026 Size: 7006 SalI G'TCGA_C Cuts at: 1936 4348 1936 Size: 2412 4594 SanDI GG'GwC_CC Cuts at: 2758 2758 Size: 7006 SbfI CC_TGCA'GG Cuts at: 4260 4358 4260 Size: 98 6908 ScaI AGT'ACT Cuts at: 3915 5254 6966 3915 Size: 1339 1712 3955 Fragments arranged by size: 3955 1712 1339 SfcI C'TryA_G Cuts at: 826 1045 2456 2699 2750 4064 4256 4354 5489 Size: 219 1411 243 51 1314 192 98 1135 Cuts at: 5489 6170 6361 826 Size: 681 191 1471 Fragments arranged by size: 1471 1411 1314 1135 681 243 219 192 191 98 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7006 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7006 SmaI CCC'GGG Cuts at: 1872 4333 1872 Size: 2461 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4086 5110 6011 6273 820 Size: 197 926 2143 1024 901 262 1553 Fragments arranged by size: 2143 1553 1024 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4797 493 Size: 4304 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7006 SphI G_CATG'C Cuts at: 1132 4364 1132 Size: 3232 3774 SspI AAT'ATT Cuts at: 5 52 4930 5 Size: 47 4878 2081 Fragments arranged by size: 4878 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7006 StyI C'CwwG_G Cuts at: 513 1065 2004 2580 2762 2896 3427 4445 513 Size: 552 939 576 182 134 531 1018 3074 Fragments arranged by size: 3074 1018 939 576 552 531 182 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3494 3666 4246 5033 5050 5203 5388 Size: 1255 1914 172 580 787 17 153 185 Cuts at: 5388 5833 325 Size: 445 1498 Fragments arranged by size: 1914 1498 1255 787 580 445 185 172 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2164 3526 3913 Size: 274 80 33 51 1443 187 1362 387 Cuts at: 3913 5252 6964 96 Size: 1339 1712 138 Fragments arranged by size: 1712 1443 1362 1339 387 274 187 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2505 2537 3077 3105 3109 3137 3291 3323 3340 Size: 32 540 28 4 28 154 32 17 Cuts at: 3340 3372 6966 6998 2505 Size: 32 3594 32 2513 Fragments arranged by size: 3594 2513 540 154 32 32 32 32 28 28 17 4 Tth111I GACn'n_nGTC Cuts at: 1316 3669 3850 1316 Size: 2353 181 4472 Fragments arranged by size: 4472 2353 181 XbaI T'CTAG_A Cuts at: 4342 4342 Size: 7006 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7006 XmaI C'CCGG_G Cuts at: 1870 4331 1870 Size: 2461 4545 XmnI GAAnn'nnTTC Cuts at: 5135 5135 Size: 7006 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4814 276 Size: 53 83 186 936 3280 2468 Fragments arranged by size: 3280 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI