(Linear) (Six-base) MAPSORT of: KIEE6705.seq Check: 4593 from: 1 to: 2301 KIEE6705.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2244 2301 Size: 2244 57 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1267 2301 Size: 1267 1034 AflIII A'CryG_T Cuts at: 0 65 2301 Size: 65 2236 AleI CACnn'nnGTG Cuts at: 0 799 2301 Size: 799 1502 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 692 709 2301 Size: 692 17 1592 Fragments arranged by size: 1592 692 17 AlwNI CAG_nnn'CTG Cuts at: 0 1839 2297 2301 Size: 1839 458 4 Fragments arranged by size: 1839 458 4 ApaI G_GGCC'C Cuts at: 0 1625 2301 Size: 1625 676 ApoI r'AATT_y Cuts at: 0 1943 2087 2301 Size: 1943 144 214 Fragments arranged by size: 1943 214 144 AvaI C'yCGr_G Cuts at: 0 1238 2032 2301 Size: 1238 794 269 Fragments arranged by size: 1238 794 269 BanI G'GyrC_C Cuts at: 0 2064 2301 Size: 2064 237 BanII G_rGCy'C Cuts at: 0 1625 2301 Size: 1625 676 BbsI GAAGACnn'nnnn_ Cuts at: 0 460 555 1700 2301 Size: 460 95 1145 601 Fragments arranged by size: 1145 601 460 95 BciVI GTATCCnnnnn_n' Cuts at: 0 1950 2301 Size: 1950 351 BclI T'GATC_A Cuts at: 0 497 1062 2301 Size: 497 565 1239 Fragments arranged by size: 1239 565 497 BglII A'GATC_T Cuts at: 0 1762 2301 Size: 1762 539 BlpI GC'TnA_GC Cuts at: 0 532 2301 Size: 532 1769 Bme1580I G_kGCm'C Cuts at: 0 838 1059 1625 2301 Size: 838 221 566 676 Fragments arranged by size: 838 676 566 221 BmrI ACTGGGnnnn_n' Cuts at: 0 330 1839 1995 2301 Size: 330 1509 156 306 Fragments arranged by size: 1509 330 306 156 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1781 2301 Size: 1781 520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 106 269 436 461 850 896 1127 Size: 34 72 163 167 25 389 46 231 Cuts at: 1127 1452 2240 2274 2301 Size: 325 788 34 27 Fragments arranged by size: 788 389 325 231 167 163 72 46 34 34 27 25 Bpu10I CC'TnA_GC Cuts at: 0 1534 2301 Size: 1534 767 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2066 2301 Size: 2066 235 BsaI GGTCTCn'nnnn_ Cuts at: 0 774 1984 2301 Size: 774 1210 317 Fragments arranged by size: 1210 774 317 BsaAI yAC'GTr Cuts at: 0 357 1116 2301 Size: 357 759 1185 Fragments arranged by size: 1185 759 357 BsaWI w'CCGG_w Cuts at: 0 1634 2301 Size: 1634 667 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1072 1100 1102 1130 1867 1897 2301 Size: 1072 28 2 28 737 30 404 Fragments arranged by size: 1072 737 404 30 28 28 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1448 1630 2301 Size: 1448 182 671 Fragments arranged by size: 1448 671 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 538 638 2301 Size: 538 100 1663 Fragments arranged by size: 1663 538 100 BsiHKAI G_wGCw'C Cuts at: 0 375 671 1634 2226 2301 Size: 375 296 963 592 75 Fragments arranged by size: 963 592 375 296 75 BsmI GAATG_Cn' Cuts at: 0 1928 2301 Size: 1928 373 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1473 2301 Size: 1473 828 Bsp1286I G_dGCh'C Cuts at: 0 375 671 838 1059 1625 1634 2226 2301 Size: 375 296 167 221 566 9 592 75 Fragments arranged by size: 592 566 375 296 221 167 75 9 BspHI T'CATG_A Cuts at: 0 525 974 1856 2301 Size: 525 449 882 445 Fragments arranged by size: 882 525 449 445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 327 510 660 2244 2301 Size: 327 183 150 1584 57 Fragments arranged by size: 1584 327 183 150 57 BsrBI CCG'CTC Cuts at: 0 1606 2301 Size: 1606 695 BsrDI GCAATG_nn' Cuts at: 0 812 2301 Size: 812 1489 BsrFI r'CCGG_y Cuts at: 0 80 2301 Size: 80 2221 BsrGI T'GTAC_A Cuts at: 0 159 1521 2301 Size: 159 1362 780 Fragments arranged by size: 1362 780 159 BssSI C'ACGA_G Cuts at: 0 1332 2301 Size: 1332 969 BstAPI GCAn_nnn'nTGC Cuts at: 0 652 1213 2058 2301 Size: 652 561 845 243 Fragments arranged by size: 845 652 561 243 BstXI CCAn_nnnn'nTGG Cuts at: 0 84 801 898 2301 Size: 84 717 97 1403 Fragments arranged by size: 1403 717 97 84 BstYI r'GATC_y Cuts at: 0 1359 1762 2301 Size: 1359 403 539 Fragments arranged by size: 1359 539 403 BtgI C'CryG_G Cuts at: 0 757 2301 Size: 757 1544 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1485 2301 Size: 1485 816 BtsI GCAGTG_nn' Cuts at: 0 1032 1580 2301 Size: 1032 548 721 Fragments arranged by size: 1032 721 548 EaeI y'GGCC_r Cuts at: 0 760 2301 Size: 760 1541 EarI CTCTTCn'nnn_ Cuts at: 0 772 1153 2301 Size: 772 381 1148 Fragments arranged by size: 1148 772 381 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 106 269 436 461 850 896 1127 Size: 34 72 163 167 25 389 46 231 Cuts at: 1127 1267 1452 2240 2274 2301 Size: 140 185 788 34 27 Fragments arranged by size: 788 389 231 185 167 163 140 72 46 34 34 27 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 890 2059 2301 Size: 890 1169 242 Fragments arranged by size: 1169 890 242 EcoO109I rG'GnC_Cy Cuts at: 0 753 880 895 1621 1836 2301 Size: 753 127 15 726 215 465 Fragments arranged by size: 753 726 465 215 127 15 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 445 2301 Size: 445 1856 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 845 877 1351 1383 1424 1456 1640 1672 Size: 845 32 474 32 41 32 184 32 Cuts at: 1672 1789 1821 2213 2245 2301 Size: 117 32 392 32 56 Fragments arranged by size: 845 474 392 184 117 56 41 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 405 2301 Size: 405 1896 MfeI C'AATT_G Cuts at: 0 347 2301 Size: 347 1954 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 40 891 1683 2301 Size: 40 851 792 618 Fragments arranged by size: 851 792 618 40 MscI TGG'CCA Cuts at: 0 762 2301 Size: 762 1539 MslI CAynn'nnrTG Cuts at: 0 634 799 1489 2301 Size: 634 165 690 812 Fragments arranged by size: 812 690 634 165 MspA1I CmG'CkG Cuts at: 0 1021 2301 Size: 1021 1280 NaeI GCC'GGC Cuts at: 0 82 2301 Size: 82 2219 NcoI C'CATG_G Cuts at: 0 757 2301 Size: 757 1544 NgoMIV G'CCGG_C Cuts at: 0 80 2301 Size: 80 2221 NspI r_CATG'y Cuts at: 0 413 2031 2301 Size: 413 1618 270 Fragments arranged by size: 1618 413 270 PflMI CCAn_nnn'nTGG Cuts at: 0 292 2301 Size: 292 2009 PpuMI rG'GwC_Cy Cuts at: 0 753 880 1836 2301 Size: 753 127 956 465 Fragments arranged by size: 956 753 465 127 PspOMI G'GGCC_C Cuts at: 0 1621 2301 Size: 1621 680 PstI C_TGCA'G Cuts at: 0 455 698 2063 2255 2301 Size: 455 243 1365 192 46 Fragments arranged by size: 1365 455 243 192 46 PvuII CAG'CTG Cuts at: 0 1021 2301 Size: 1021 1280 SanDI GG'GwC_CC Cuts at: 0 753 2301 Size: 753 1548 SbfI CC_TGCA'GG Cuts at: 0 2255 2301 Size: 2255 46 ScaI AGT'ACT Cuts at: 0 1910 2301 Size: 1910 391 SfcI C'TryA_G Cuts at: 0 451 694 745 2059 2251 2301 Size: 451 243 51 1314 192 50 Fragments arranged by size: 1314 451 243 192 51 50 SmlI C'TyrA_G Cuts at: 0 2081 2301 Size: 2081 220 StyI C'CwwG_G Cuts at: 0 575 757 891 1422 2301 Size: 575 182 134 531 879 Fragments arranged by size: 879 575 531 182 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1489 1661 2241 2301 Size: 1489 172 580 60 Fragments arranged by size: 1489 580 172 60 TatI w'GTAC_w Cuts at: 0 159 1521 1908 2301 Size: 159 1362 387 393 Fragments arranged by size: 1362 393 387 159 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 500 532 1072 1100 1104 1132 1286 1318 Size: 500 32 540 28 4 28 154 32 Cuts at: 1318 1335 1367 2301 Size: 17 32 934 Fragments arranged by size: 934 540 500 154 32 32 32 28 28 17 4 Tth111I GACn'n_nGTC Cuts at: 0 1664 1845 2301 Size: 1664 181 456 Fragments arranged by size: 1664 456 181 Enzymes that do cut: AarI AcuI AflIII AleI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PpuMI PspOMI PstI PvuII SanDI SbfI ScaI SfcI SmlI StyI TaqII TatI TstI Tth111I Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaBI BsaHI BseYI BsiEI BsiWI BspEI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XcmI XhoI XmaI XmnI ZraI