Restriction map for
pFN21AB6369
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BciVI BclI BcnI BfrI BglI
BlnI BmyI BpmI BsaAI BsaHI
BsaI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssKI
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MslI MunI MvaI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScrFI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccIII 21 TCCGGA T^CCGGA
AciI 203 CCGC CCGC(-3/-1)
BalI 715 TGGCCA TGG^CCA
BcgI 153 CGATTCAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 306 AGATCT A^GATCT
BlpI 163 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 163 GCTCAGC GC^T[ACGT]AGC
BsaBI 13 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 536 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 21 TCCGGA [AT]^CCGG[AT]
BseAI 21 TCCGGA T^CCGGA
BspEI 21 TCCGGA T^CCGGA
CelII 163 GCTCAGC GC^T[ACGT]AGC
EaeI 715 TGGCCA [CT]^GGCC[GA]
EarI 283 GAAGAG CTCTTC(1/4)
EcoT14I 536 CCTTGG C^C[AT][AT]GG
HaeIII 716 GGCC GG^CC
HapII 22 CCGG C^CGG
HindIII 794 AAGCTT A^AGCTT
HpaII 22 CCGG C^CGG
HphI 149 TCACC GGTGA(8/7)
Ksp632I 283 GAAGAG CTCTTC(1/4)
MaeII 260 ACGT A^CGT
MaeIII 49 GTTAC ^GT[ACGT]AC
MamI 13 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 715 TGGCCA TGG^CCA
MroI 21 TCCGGA T^CCGGA
MscI 715 TGGCCA TGG^CCA
MspA1I 203 CCGCTG C[AC]G^C[GT]G
MspI 22 CCGG C^CGG
MwoI 732 GCTGCTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 689 CATATG CA^TATG
PleI 334 GAGTC GAGTC(4/5)
ScaI 28 AGTACT AGT^ACT
SfcI 32 CTACAG C^T[GA][CT]AG
StyI 536 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 12 enzymes) #
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Asp700I 238 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
256 GAAGACGTTC
BfaI 16 CTAG C^TAG
550 CTAG
BsmAI 300 GAGAC GTCTC(1/5)
759 GAGAC
BstF5I 763 CATCC GGATG(2/0)
387 GGATG
FokI 763 CATCC GGATG(9/13)
387 GGATG
MaeI 16 CTAG C^TAG
550 CTAG
MnlI 565 CCTC CCTC(7/6)
10 GAGG
NlaIII 637 CATG CATG^
682 CATG
SmlI 528 CTTGAG C^T[CT][GA]AG
756 CTTGAG
TspRI 429 CAGTG CA[GC]TG[ACGT][ACGT]^
632 CACTG
XbaI 15 TCTAGA T^CTAGA
549 TCTAGA
XmnI 238 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
256 GAAGACGTTC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AfaI AluI AlwI BbsI BbvI
BpuAI BstYI DdeI DpnII Fnu4HI
HinfI ItaI MboI MboII MflI
MseI NdeII RsaI Sau3AI TfiI
Tru9I TseI Tsp509I XhoII