################################## # NO CUT ENZYMES ( 203 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FokI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MamI MflI MluI MluNI MroI MscI MspA1I MvaI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcsI 191 AAATTT [GA]^AATT[CT] AluI 174 AGCT AG^CT AlwI 318 GATCC GGATC(4/5) ApoI 191 AAATTT [GA]^AATT[CT] AspHI 173 GAGCTC G[AT]GC[AT]^C BanII 173 GAGCTC G[GA]GC[CT]^C BbvI 160 GCAGC GCAGC(8/12) BclI 444 TGATCA T^GATCA BfaI 170 CTAG C^TAG BmyI 173 GAGCTC G[AGT]GC[ACT]^C BpmI 215 CTCCAG CTGGAG(16/14) BsaWI 229 ACCGGA [AT]^CCGG[AT] BsiHKAI 173 GAGCTC G[AT]GC[AT]^C BsiYI 230 CCGGAGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 230 CCGGAGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 436 GAGAC GTCTC(1/5) BsmFI 149 GGGAC GGGAC(10/14) Bsp1286I 173 GAGCTC G[AGT]GC[ACT]^C BspHI 177 TCATGA T^CATGA BsrDI 76 GCAATG GCAATG(2/0) BstXI 65 CCACTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 68 CTGAG C^T[ACGT]AG FbaI 444 TGATCA T^GATCA Fnu4HI 160 GCAGC GC^[ACGT]GC HapII 230 CCGG C^CGG HpaII 230 CCGG C^CGG ItaI 160 GCAGC GC^[ACGT]GC MaeI 170 CTAG C^TAG MaeII 401 ACGT A^CGT MfeI 38 CAATTG C^AATTG MspI 230 CCGG C^CGG MunI 38 CAATTG C^AATTG NdeI 155 CATATG CA^TATG NlaIII 178 CATG CATG^ RcaI 177 TCATGA T^CATGA SacI 173 GAGCTC GAGCT^C TseI 160 GCAGC G^C[AT]GC Tsp45I 103 GTCAC ^GT[GC]AC TspRI 66 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AfaI 62 GTAC GT^AC 241 GTAC DpnII 318 GATC ^GATC 445 GATC EarI 93 CTCTTC CTCTTC(1/4) 220 GAAGAG HphI 294 GGTGA GGTGA(8/7) 388 TCACC Ksp632I 93 CTCTTC CTCTTC(1/4) 220 GAAGAG MaeIII 103 GTCAC ^GT[ACGT]AC 113 GTTAC MboI 318 GATC ^GATC 445 GATC MslI 66 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 396 CACAGACGTG NdeII 318 GATC ^GATC 445 GATC RsaI 62 GTAC GT^AC 241 GTAC Sau3AI 318 GATC ^GATC 445 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### MboII MnlI MseI Tru9I Tsp509I