(Circular) (Six-base) MAPSORT of: pFN21AE6129.seq Check: 5706 from: 1 to: 5155 pFN21AE6129 5155 bp With 182 enzymes: SgfI * December 8, 2014 13:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2965 278 Size: 53 83 186 936 1429 2468 Fragments arranged by size: 2468 1429 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2498 1937 Size: 561 4594 Acc65I G'GTAC_C Cuts at: 2476 2476 Size: 5155 AclI AA'CG_TT Cuts at: 1563 3282 3655 1563 Size: 1719 373 3063 Fragments arranged by size: 3063 1719 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2088 3218 4602 4870 1632 Size: 456 1130 1384 268 1917 Fragments arranged by size: 1917 1384 1130 456 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4958 AflIII A'CryG_T Cuts at: 1203 4054 5031 1203 Size: 2851 977 1327 Fragments arranged by size: 2851 1327 977 AgeI A'CCGG_T Cuts at: 3986 3986 Size: 5155 AhdI GACnn_n'nnGTC Cuts at: 3884 3884 Size: 5155 AleI CACnn'nnGTG Cuts at: 1166 2075 2405 1166 Size: 909 330 3916 Fragments arranged by size: 3916 909 330 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5155 AlwNI CAG_nnn'CTG Cuts at: 2898 4470 2898 Size: 1572 3583 ApaLI G'TGCA_C Cuts at: 3212 4368 3212 Size: 1156 3999 ApoI r'AATT_y Cuts at: 1493 2196 2464 2690 2842 4951 1493 Size: 703 268 226 152 2109 1697 Fragments arranged by size: 2109 1697 703 268 226 152 AseI AT'TA_AT Cuts at: 160 1032 3709 160 Size: 872 2677 1606 Fragments arranged by size: 2677 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5155 AvaI C'yCGr_G Cuts at: 1870 1943 2480 1870 Size: 73 537 4545 Fragments arranged by size: 4545 537 73 BamHI G'GATC_C Cuts at: 2485 2852 2485 Size: 367 4788 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2476 3931 618 Size: 325 217 627 689 1455 1842 Fragments arranged by size: 1842 1455 689 627 325 217 BanII G_rGCy'C Cuts at: 1391 2182 2474 1391 Size: 791 292 4072 Fragments arranged by size: 4072 791 292 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4184 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3346 3380 1903 Size: 34 1409 34 3678 Fragments arranged by size: 3678 1409 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3047 4257 3047 Size: 1210 3945 BclI T'GATC_A Cuts at: 1548 2449 1548 Size: 901 4254 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3766 136 Size: 107 122 71 631 605 2094 1525 Fragments arranged by size: 2094 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5150 5150 Size: 5155 BlpI GC'TnA_GC Cuts at: 2572 2572 Size: 5155 Bme1580I G_kGCm'C Cuts at: 1790 3216 4372 1790 Size: 1426 1156 2573 Fragments arranged by size: 2573 1426 1156 BmgBI CAC'GTC Cuts at: 1318 2407 1318 Size: 1089 4066 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2886 3844 442 Size: 416 53 83 399 24 1469 958 1753 Fragments arranged by size: 1753 1469 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5155 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2205 3815 1239 Size: 966 1610 2579 Fragments arranged by size: 2579 1610 966 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3280 4145 4443 3280 Size: 865 298 3992 Fragments arranged by size: 3992 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3818 882 Size: 617 2319 2219 Fragments arranged by size: 2319 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2946 493 Size: 711 1742 2702 Fragments arranged by size: 2702 1742 711 BsaBI GATnn'nnATC Cuts at: 5149 5149 Size: 5155 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2962 3344 3979 275 Size: 53 83 186 936 1429 382 635 1451 Fragments arranged by size: 1451 1429 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2234 3588 3986 4260 4407 4818 2234 Size: 1354 398 274 147 411 2571 Fragments arranged by size: 2571 1354 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2229 2259 1178 Size: 30 165 30 826 30 4074 Fragments arranged by size: 4074 826 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5155 BseYI C'CCAG_C Cuts at: 4358 4854 4358 Size: 496 4659 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4582 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2605 3366 4394 1540 Size: 275 186 604 761 1028 2301 Fragments arranged by size: 2301 1028 761 604 275 186 BsiHKAI G_wGCw'C Cuts at: 2182 3216 3301 4372 2182 Size: 1034 85 1071 2965 Fragments arranged by size: 2965 1071 1034 85 BsmI GAATG_Cn' Cuts at: 2666 2759 2666 Size: 93 5062 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2182 2474 3216 3301 4372 1391 Size: 399 392 292 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 392 292 85 BspEI T'CCGG_A Cuts at: 4818 4818 Size: 5155 BspHI T'CATG_A Cuts at: 2182 3044 4774 2182 Size: 862 1730 2563 Fragments arranged by size: 2563 1730 862 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2510 844 Size: 1666 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3042 1394 Size: 429 1219 3507 Fragments arranged by size: 3507 1219 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2088 3650 3824 65 Size: 1183 840 1562 174 1396 Fragments arranged by size: 1562 1396 1183 840 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3799 3986 1700 Size: 110 1989 187 2869 Fragments arranged by size: 2869 1989 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5155 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5155 BssSI C'ACGA_G Cuts at: 3215 4227 3215 Size: 1012 4143 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5155 BstBI TT'CG_AA Cuts at: 3959 3959 Size: 5155 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5155 BstXI CCAn_nnnn'nTGG Cuts at: 2077 2077 Size: 5155 BstYI r'GATC_y Cuts at: 1972 2485 2852 3237 3254 4706 4792 4804 5150 Size: 513 367 385 17 1452 86 12 346 Cuts at: 5150 1972 Size: 1977 Fragments arranged by size: 1977 1452 513 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4603 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4416 Fragments arranged by size: 4416 683 56 BtsI GCAGTG_nn' Cuts at: 2671 3465 3485 2671 Size: 794 20 4341 Fragments arranged by size: 4341 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5155 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5120 DraI TTT'AAA Cuts at: 2459 2459 Size: 5155 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4162 809 Size: 507 2846 1802 Fragments arranged by size: 2846 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2602 3491 5120 Size: 54 1425 50 275 18 772 889 1629 Cuts at: 5120 8 Size: 43 Fragments arranged by size: 1629 1425 889 772 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2602 1537 Size: 275 790 4090 Fragments arranged by size: 4090 790 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2104 2220 3085 1353 Size: 751 116 865 3423 Fragments arranged by size: 3423 865 751 116 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3742 4116 4262 387 Size: 1064 138 2153 374 146 1280 Fragments arranged by size: 2153 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2180 2180 Size: 5155 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 2205 3218 3815 4602 4870 1239 Size: 393 456 117 1013 597 787 268 1524 Fragments arranged by size: 1524 1013 787 597 456 393 268 117 EcoRI G'AATT_C Cuts at: 2464 2842 2464 Size: 378 4777 FspI TGC'GCA Cuts at: 3661 3661 Size: 5155 HaeII r_GCGC'y Cuts at: 1678 4302 1678 Size: 2624 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2434 2466 3796 3828 3870 Size: 32 135 32 862 32 1330 32 42 Cuts at: 3870 3902 1373 Size: 32 2626 Fragments arranged by size: 2626 1330 862 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2499 2745 669 Size: 988 281 561 246 3079 Fragments arranged by size: 3079 988 561 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5155 HpaI GTT'AAC Cuts at: 2745 2745 Size: 5155 KpnI G_GTAC'C Cuts at: 2480 2480 Size: 5155 MfeI C'AATT_G Cuts at: 2043 2754 2043 Size: 711 4444 MluI A'CGCG_T Cuts at: 5031 5031 Size: 5155 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4269 4453 1305 Size: 2964 184 2007 Fragments arranged by size: 2964 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3333 Fragments arranged by size: 3333 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2075 2405 3113 3472 3631 518 Size: 648 909 330 708 359 159 2042 Fragments arranged by size: 2042 909 708 648 359 330 159 MspA1I CmG'CkG Cuts at: 1606 2571 3248 4396 4641 1606 Size: 965 677 1148 245 2120 Fragments arranged by size: 2120 1148 965 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4603 NdeI CA'TA_TG Cuts at: 387 2161 387 Size: 1774 3381 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5155 NotI GC'GGCC_GC Cuts at: 2602 2602 Size: 5155 NspI r_CATG'y Cuts at: 1132 1229 2513 4058 1132 Size: 97 1284 1545 2229 Fragments arranged by size: 2229 1545 1284 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5155 PciI A'CATG_T Cuts at: 4054 4054 Size: 5155 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5155 PmeI GTTT'AAAC Cuts at: 2459 2459 Size: 5155 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3224 3256 4737 4769 3224 Size: 32 1481 32 3610 Fragments arranged by size: 3610 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5155 PsiI TTA'TAA Cuts at: 2725 2725 Size: 5155 PstI C_TGCA'G Cuts at: 830 2507 830 Size: 1677 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5155 SacI G_AGCT'C Cuts at: 2182 2182 Size: 5155 SalI G'TCGA_C Cuts at: 1936 2497 1936 Size: 561 4594 SbfI CC_TGCA'GG Cuts at: 2507 2507 Size: 5155 ScaI AGT'ACT Cuts at: 3403 5115 3403 Size: 1712 3443 SfcI C'TryA_G Cuts at: 826 1045 2503 3638 4319 4510 826 Size: 219 1458 1135 681 191 1471 Fragments arranged by size: 1471 1458 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5155 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5155 SmaI CCC'GGG Cuts at: 1872 2482 1872 Size: 610 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3259 4160 4422 820 Size: 197 926 1316 901 262 1553 Fragments arranged by size: 1553 1316 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2946 493 Size: 2453 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5155 SphI G_CATG'C Cuts at: 1132 2513 1132 Size: 1381 3774 SspI AAT'ATT Cuts at: 5 52 3079 5 Size: 47 3027 2081 Fragments arranged by size: 3027 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5155 StyI C'CwwG_G Cuts at: 513 1065 2594 513 Size: 552 1529 3074 Fragments arranged by size: 3074 1529 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3182 3199 3352 3537 3982 325 Size: 1255 1602 17 153 185 445 1498 Fragments arranged by size: 1602 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3401 5113 96 Size: 274 80 33 51 1443 1424 1712 138 Fragments arranged by size: 1712 1443 1424 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5115 5147 5115 Size: 32 5123 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5155 XbaI T'CTAG_A Cuts at: 2491 2491 Size: 5155 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5155 XmaI C'CCGG_G Cuts at: 1870 2480 1870 Size: 610 4545 XmnI GAAnn'nnTTC Cuts at: 3284 3284 Size: 5155 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2963 276 Size: 53 83 186 936 1429 2468 Fragments arranged by size: 2468 1429 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI