################################## # NO CUT ENZYMES ( 225 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I TseI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AcsI 276 GAATTT [GA]^AATT[CT] AfaI 153 GTAC GT^AC ApoI 276 GAATTT [GA]^AATT[CT] AspI 83 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BssKI 103 CCTGG ^CC[ACGT]GG BstNI 103 CCTGG CC^[AT]GG Cac8I 240 GCAAGC GC[ACGT]^[ACGT]GC Cfr13I 100 GGCCC G^G[ACGT]CC DraII 99 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 99 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRII 103 CCTGG ^CC[AT]GG EcoT14I 26 CCTTGG C^C[AT][AT]GG HaeIII 100 GGCC GG^CC HgaI 83 GACGC GACGC(5/10) MvaI 103 CCTGG CC^[AT]GG PflFI 83 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC RsaI 153 GTAC GT^AC Sau96I 100 GGCCC G^G[ACGT]CC ScrFI 103 CCTGG CC^[ACGT]GG SfaNI 218 GATGC GCATC(5/9) StyI 26 CCTTGG C^C[AT][AT]GG TaqI 235 TCGA T^CGA Tsp45I 170 GTGAC ^GT[GC]AC Tsp509I 277 AATT ^AATT TspRI 116 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 83 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 235 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 5 enzymes) # ################################### MaeIII 161 GTAAC ^GT[ACGT]AC 170 GTGAC MnlI 143 CCTC CCTC(7/6) 80 GAGG MwoI 86 GCTGTCTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 240 GCAAGCAAGGC NlaIII 15 CATG CATG^ 69 CATG SfcI 20 CTATAG C^T[GA][CT]AG 47 CTACAG ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### BsaJI