################################## # NO CUT ENZYMES ( 206 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MamI MboII MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AfaI 108 GTAC GT^AC AlwNI 14 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 138 GGACC G^G[AT]CC BanII 159 GAGCCC G[GA]GC[CT]^C BclI 224 TGATCA T^GATCA BmyI 159 GAGCCC G[AGT]GC[ACT]^C BsaJI 176 CCGAGG C^C[ACGT][ACGT]GG BseRI 28 CTCCTC GAGGAG(10/8) BsmAI 66 GAGAC GTCTC(1/5) Bsp1286I 159 GAGCCC G[AGT]GC[ACT]^C BsrI 246 ACTGG ACTGG(1/-1) BssKI 96 CCTGG ^CC[ACGT]GG BstNI 96 CCTGG CC^[AT]GG Cfr13I 138 GGACC G^G[ACGT]CC DdeI 104 CTCAG C^T[ACGT]AG DpnII 225 GATC ^GATC DraII 137 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO109I 137 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 96 CCTGG ^CC[AT]GG FbaI 224 TGATCA T^GATCA HincII 250 GTTAAC GT[CT]^[GA]AC HindII 250 GTTAAC GT[CT]^[GA]AC HpaI 250 GTTAAC GTT^AAC HphI 32 TCACC GGTGA(8/7) MaeIII 121 GTGAC ^GT[ACGT]AC MboI 225 GATC ^GATC MseI 251 TTAA T^TAA MspA1I 211 CAGCTG C[AC]G^C[GT]G MvaI 96 CCTGG CC^[AT]GG NdeII 225 GATC ^GATC NlaIII 83 CATG CATG^ NlaIV 78 GGAGCC GG[ACGT]^[ACGT]CC PpuMI 137 AGGACCT [GA]G^G[AT]CC[CT] PstI 39 CTGCAG CTGCA^G PvuII 211 CAGCTG CAG^CTG RsaI 108 GTAC GT^AC Sau3AI 225 GATC ^GATC Sau96I 138 GGACC G^G[ACGT]CC ScaI 107 AGTACT AGT^ACT ScrFI 96 CCTGG CC^[ACGT]GG SfcI 39 CTGCAG C^T[GA][CT]AG SmlI 258 CTTGAG C^T[CT][GA]AG Tru9I 251 TTAA T^TAA Tsp45I 121 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 3 enzymes) # ################################### Cac8I 9 GCTCGC GC[ACGT]^[ACGT]GC 288 GCCTGC HaeIII 174 GGCC GG^CC 180 GGCC MnlI 30 CCTC CCTC(7/6) 178 GAGG ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### AluI BbvI Fnu4HI ItaI TseI