################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FokI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MfeI MflI MluI MluNI MroI MscI MseI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AclI 59 AACGTT AA^CGTT AfaI 43 GTAC GT^AC AlwI 127 GATCC GGATC(4/5) AvaII 302 GGACC G^G[AT]CC BclI 132 TGATCA T^GATCA BpmI 201 CTCCAG CTGGAG(16/14) BsaBI 127 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 79 GAGGAG GAGGAG(10/8) BsgI 223 GTGCAG GTGCAG(16/14) BsmAI 288 GAGAC GTCTC(1/5) CfoI 153 GCGC GCG^C Cfr13I 302 GGACC G^G[ACGT]CC DraII 301 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 119 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 426 TGGCCG [CT]^GGCC[GA] EarI 400 GAAGAG CTCTTC(1/4) EcoO109I 301 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 352 GAATTC G^AATTC EcoT14I 347 CCAAGG C^C[AT][AT]GG FbaI 132 TGATCA T^GATCA HaeII 152 AGCGCC [GA]GCGC^[CT] HhaI 153 GCGC GCG^C HinP1I 153 GCGC G^CGC Ksp632I 400 GAAGAG CTCTTC(1/4) MamI 127 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 436 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 51 CAGCTG C[AC]G^C[GT]G MwoI 103 GCTTCCCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 301 GGGACC GG[ACGT]^[ACGT]CC PleI 199 GACTC GAGTC(4/5) PpuMI 301 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 59 AACGTT AA^CGTT PvuII 51 CAGCTG CAG^CTG RsaI 43 GTAC GT^AC Sau96I 302 GGACC G^G[ACGT]CC SfcI 183 CTACAG C^T[GA][CT]AG StyI 347 CCAAGG C^C[AT][AT]GG TfiI 513 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 429 CCGC CCGC(-3/-1) 488 CCGC AcsI 280 GAATTT [GA]^AATT[CT] 352 GAATTC ApoI 280 GAATTT [GA]^AATT[CT] 352 GAATTC BbvI 50 GCAGC GCAGC(8/12) 343 GCTGC BmyI 165 GGGCAC G[AGT]GC[ACT]^C 209 GTGCCC BsiYI 156 CCATTCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 396 CCCAGAAGAGG BslI 156 CCATTCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 396 CCCAGAAGAGG BsmFI 301 GGGAC GGGAC(10/14) 494 GGGAC Bsp1286I 165 GGGCAC G[AGT]GC[ACT]^C 209 GTGCCC BsrDI 261 CATTGC GCAATG(2/0) 357 CATTGC Cac8I 53 GCTGGC GC[ACGT]^[ACGT]GC 207 GCGTGC DdeI 99 CTCAG C^T[ACGT]AG 121 CTCAG HinfI 199 GACTC G^A[ACGT]TC 513 GATTC HphI 369 GGTGA GGTGA(8/7) 173 TCACC MaeII 60 ACGT A^CGT 270 ACGT MaeIII 179 GTGAC ^GT[ACGT]AC 272 GTCAC MboII 400 GAAGA GAAGA(8/7) 418 GAAGA NlaIII 292 CATG CATG^ 436 CATG NspI 291 ACATGC [GA]CATG^[CT] 435 ACATGC TseI 50 GCAGC G^C[AT]GC 343 GCTGC Tsp45I 179 GTGAC ^GT[GC]AC 272 GTCAC Tsp509I 281 AATT ^AATT 353 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AluI BsaJI BsrI BssKI BstNI DpnII EcoRII Fnu4HI HaeIII ItaI MboI MnlI MvaI NdeII Sau3AI ScrFI TspRI