################################## # NO CUT ENZYMES ( 211 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AcsI 197 AAATTT [GA]^AATT[CT] AlwI 117 GGATC GGATC(4/5) ApoI 197 AAATTT [GA]^AATT[CT] AvaII 303 GGACC G^G[AT]CC BbvI 17 GCAGC GCAGC(8/12) BcgI 278 GCAAGTATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaJI 12 CCTGGG C^C[ACGT][ACGT]GG BsiYI 76 CCTAATGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 76 CCTAATGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 172 GAGAC GTCTC(1/5) BsrDI 53 GCAATG GCAATG(2/0) BstYI 117 GGATCT [GA]^GATC[CT] Cfr13I 303 GGACC G^G[ACGT]CC DraII 302 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 302 AGGACCC [GA]G^G[ACGT]CC[CT] Fnu4HI 17 GCAGC GC^[ACGT]GC ItaI 17 GCAGC GC^[ACGT]GC MaeIII 43 GTGAC ^GT[ACGT]AC MflI 117 GGATCT [GA]^GATC[CT] MslI 36 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIV 303 GGACCC GG[ACGT]^[ACGT]CC NspI 175 ACATGC [GA]CATG^[CT] PpuMI 302 AGGACCC [GA]G^G[AT]CC[CT] Sau96I 303 GGACC G^G[ACGT]CC SfaNI 58 GCATC GCATC(5/9) TseI 17 GCAGC G^C[AT]GC Tsp45I 43 GTGAC ^GT[GC]AC Tsp509I 198 AATT ^AATT TspRI 327 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 104 CCAGGAAATGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 117 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### DpnII 27 GATC ^GATC 118 GATC EcoT22I 56 ATGCAT ATGCA^T 111 ATGCAT HinfI 97 GATTC G^A[ACGT]TC 257 GAATC MboI 27 GATC ^GATC 118 GATC MboII 297 GAAGA GAAGA(8/7) 260 TCTTC MnlI 82 GAGG CCTC(7/6) 271 GAGG NdeII 27 GATC ^GATC 118 GATC NsiI 56 ATGCAT ATGCA^T 111 ATGCAT Sau3AI 27 GATC ^GATC 118 GATC TfiI 97 GATTC G^A[AT]TC 257 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### BssKI BstNI EcoRII MvaI NlaIII ScrFI