##################################
# NO CUT ENZYMES (  219 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DpnII       DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HaeIII      HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       HphI        
ItaI        KasI        KpnI        Ksp632I     KspI        
MaeII       MaeIII      MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TseI        Tsp45I      TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI          239  GTCTAC               GT^[AC][GT]AC
AfaI          282  GTAC                 GT^AC
AvaII         364  GGTCC                G^G[AT]CC
BbsI          415  GAAGAC               GAAGAC(2/6)
BfaI          299  CTAG                 C^TAG
BpmI          368  CTGGAG               CTGGAG(16/14)
BpuAI         415  GAAGAC               GAAGAC(2/6)
BsmFI         245  GGGAC                GGGAC(10/14)
BsrI          387  CCAGT                ACTGG(1/-1)
BssKI         367  CCTGG                ^CC[ACGT]GG
BstF5I         90  GGATG                GGATG(2/0)
BstNI         367  CCTGG                CC^[AT]GG
Cac8I         320  GCGAGC               GC[ACGT]^[ACGT]GC
CfoI           36  GCGC                 GCG^C
Cfr13I        364  GGTCC                G^G[ACGT]CC
DdeI          107  CTCAG                C^T[ACGT]AG
EcoRII        367  CCTGG                ^CC[AT]GG
EcoRV         427  GATATC               GAT^ATC
FokI           90  GGATG                GGATG(9/13)
HhaI           36  GCGC                 GCG^C
HinP1I         36  GCGC                 G^CGC
HinfI          58  GAATC                G^A[ACGT]TC
MaeI          299  CTAG                 C^TAG
MboII         415  GAAGA                GAAGA(8/7)
MvaI          367  CCTGG                CC^[AT]GG
MwoI          289  GCAGATGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI          314  CATATG               CA^TATG
NlaIII        334  CATG                 CATG^
NspI          333  ACATGC               [GA]CATG^[CT]
RsaI          282  GTAC                 GT^AC
Sau96I        364  GGTCC                G^G[ACGT]CC
ScrFI         367  CCTGG                CC^[ACGT]GG
TfiI           58  GAATC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

DraI           82  TTTAAA               TTT^AAA
              276  TTTAAA              
MseI           83  TTAA                 T^TAA
              277  TTAA                
Tru9I          83  TTAA                 T^TAA
              277  TTAA                
Tsp509I       191  AATT                 ^AATT
              273  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

AluI        MnlI