(Circular) (Six-base) MAPSORT of: pFN21AE4529.seq Check: 3930 from: 1 to: 6445 pFN21AE4529 6445 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2936 2936 Size: 6445 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3159 4255 278 Size: 53 83 186 936 1623 1096 2468 Fragments arranged by size: 2468 1623 1096 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3788 1937 Size: 1851 4594 Acc65I G'GTAC_C Cuts at: 3766 3766 Size: 6445 AclI AA'CG_TT Cuts at: 1563 4572 4945 1563 Size: 3009 373 3063 Fragments arranged by size: 3063 3009 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2735 2755 4508 5892 6160 1632 Size: 1103 20 1753 1384 268 1917 Fragments arranged by size: 1917 1753 1384 1103 268 20 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6248 AflIII A'CryG_T Cuts at: 1203 2427 3420 5344 6321 1203 Size: 1224 993 1924 977 1327 Fragments arranged by size: 1924 1327 1224 993 977 AgeI A'CCGG_T Cuts at: 5276 5276 Size: 6445 AhdI GACnn_n'nnGTC Cuts at: 5174 5174 Size: 6445 AleI CACnn'nnGTG Cuts at: 1166 2865 1166 Size: 1699 4746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2219 1184 Size: 1035 5410 AlwNI CAG_nnn'CTG Cuts at: 2413 4188 5760 2413 Size: 1775 1572 3098 Fragments arranged by size: 3098 1775 1572 ApaI G_GGCC'C Cuts at: 3351 3351 Size: 6445 ApaLI G'TGCA_C Cuts at: 4502 5658 4502 Size: 1156 5289 ApoI r'AATT_y Cuts at: 1493 2675 3050 3754 3980 4132 6241 1493 Size: 1182 375 704 226 152 2109 1697 Fragments arranged by size: 2109 1697 1182 704 375 226 152 AseI AT'TA_AT Cuts at: 160 1032 4999 160 Size: 872 3967 1606 Fragments arranged by size: 3967 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2597 3103 3770 1870 Size: 73 120 534 506 667 4545 Fragments arranged by size: 4545 667 534 506 120 73 BamHI G'GATC_C Cuts at: 2590 2814 3775 4142 2590 Size: 224 961 367 4893 Fragments arranged by size: 4893 961 367 224 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3107 3176 3560 3766 5221 Size: 325 217 627 1320 69 384 206 1455 Cuts at: 5221 618 Size: 1842 Fragments arranged by size: 1842 1455 1320 627 384 325 217 206 69 BanII G_rGCy'C Cuts at: 1391 2259 3351 3764 1391 Size: 868 1092 413 4072 Fragments arranged by size: 4072 1092 868 413 BbeI G_GCGC'C Cuts at: 3564 3564 Size: 6445 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2127 2723 2863 928 Size: 971 228 596 140 4510 Fragments arranged by size: 4510 971 596 228 140 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4636 4670 1903 Size: 34 2699 34 3678 Fragments arranged by size: 3678 2699 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4337 5547 4337 Size: 1210 5235 BclI T'GATC_A Cuts at: 1548 2781 1548 Size: 1233 5212 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3446 5056 136 Size: 107 122 71 631 605 1774 1610 1525 Fragments arranged by size: 1774 1610 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6440 6440 Size: 6445 BlpI GC'TnA_GC Cuts at: 3533 3862 3533 Size: 329 6116 Bme1580I G_kGCm'C Cuts at: 1790 3110 3179 3351 4506 5662 1790 Size: 1320 69 172 1155 1156 2573 Fragments arranged by size: 2573 1320 1156 1155 172 69 BmgBI CAC'GTC Cuts at: 1318 2428 2911 1318 Size: 1110 483 4852 Fragments arranged by size: 4852 1110 483 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2444 2859 4176 Size: 416 53 83 399 24 1027 415 1317 Cuts at: 4176 5134 442 Size: 958 1753 Fragments arranged by size: 1753 1317 1027 958 416 415 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2635 2714 2849 5105 1239 Size: 1396 79 135 2256 2579 Fragments arranged by size: 2579 2256 1396 135 79 Bpu10I CC'TnA_GC Cuts at: 2485 2485 Size: 6445 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4570 5435 5733 4570 Size: 865 298 5282 Fragments arranged by size: 5282 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2030 2825 5108 882 Size: 617 531 795 2283 2219 Fragments arranged by size: 2283 2219 795 617 531 BsaAI yAC'GTr Cuts at: 493 1204 2527 4236 493 Size: 711 1323 1709 2702 Fragments arranged by size: 2702 1709 1323 711 BsaBI GATnn'nnATC Cuts at: 2819 3125 6439 2819 Size: 306 3314 2825 Fragments arranged by size: 3314 2825 306 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3156 3561 4252 4634 Size: 53 83 186 936 1623 405 691 382 Cuts at: 4634 5269 275 Size: 635 1451 Fragments arranged by size: 1623 1451 936 691 635 405 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4878 5276 5550 5697 6108 4878 Size: 398 274 147 411 5215 Fragments arranged by size: 5215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2363 2393 3485 3515 1178 Size: 30 165 30 960 30 1092 30 4108 Fragments arranged by size: 4108 1092 960 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2936 3617 3737 1186 Size: 1750 681 120 3894 Fragments arranged by size: 3894 1750 681 120 BseYI C'CCAG_C Cuts at: 2323 2618 3272 3343 3486 5648 6144 2323 Size: 295 654 71 143 2162 496 2624 Fragments arranged by size: 2624 2162 654 496 295 143 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2258 1163 Size: 573 522 5350 Fragments arranged by size: 5350 573 522 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3895 4656 5684 1540 Size: 275 186 1894 761 1028 2301 Fragments arranged by size: 2301 1894 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3096 3534 4506 4591 5662 3096 Size: 438 972 85 1071 3879 Fragments arranged by size: 3879 1071 972 438 85 BsmI GAATG_Cn' Cuts at: 3727 3956 4049 3727 Size: 229 93 6123 Fragments arranged by size: 6123 229 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2141 2141 Size: 6445 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 3096 3110 3179 3351 3534 3764 Size: 399 469 837 14 69 172 183 230 Cuts at: 3764 4506 4591 5662 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 837 742 469 399 230 183 172 85 69 14 BspEI T'CCGG_A Cuts at: 6108 6108 Size: 6445 BspHI T'CATG_A Cuts at: 2151 3114 4334 6064 2151 Size: 963 1220 1730 2532 Fragments arranged by size: 2532 1730 1220 963 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2936 3800 844 Size: 2092 864 3489 Fragments arranged by size: 3489 2092 864 BsrBI CCG'CTC Cuts at: 1394 1823 2044 2342 2602 2958 3043 3369 3675 Size: 429 221 298 260 356 85 326 306 Cuts at: 3675 4332 1394 Size: 657 3507 Fragments arranged by size: 3507 657 429 356 326 306 298 260 221 85 BsrDI GCAATG_nn' Cuts at: 65 1248 4940 5114 65 Size: 1183 3692 174 1396 Fragments arranged by size: 3692 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2092 2439 3557 5089 5276 1700 Size: 110 282 347 1118 1532 187 2869 Fragments arranged by size: 2869 1532 1118 347 282 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6445 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6445 BssSI C'ACGA_G Cuts at: 4505 5517 4505 Size: 1012 5433 BstAPI GCAn_nnn'nTGC Cuts at: 794 2193 3733 794 Size: 1399 1540 3506 Fragments arranged by size: 3506 1540 1399 BstBI TT'CG_AA Cuts at: 5249 5249 Size: 6445 BstEII G'GTnAC_C Cuts at: 1184 2465 1184 Size: 1281 5164 BstXI CCAn_nnnn'nTGG Cuts at: 2827 3142 2827 Size: 315 6130 BstYI r'GATC_y Cuts at: 1972 2220 2590 2751 2814 3775 4142 4527 4544 Size: 248 370 161 63 961 367 385 17 Cuts at: 4544 5996 6082 6094 6440 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 961 385 370 367 346 248 161 86 63 17 12 BtgI C'CryG_G Cuts at: 513 1065 2455 3378 3667 513 Size: 552 1390 923 289 3291 Fragments arranged by size: 3291 1390 923 552 289 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2284 3102 3232 3529 3610 487 Size: 683 56 1058 818 130 297 81 3322 Fragments arranged by size: 3322 1058 818 683 297 130 81 56 BtsI GCAGTG_nn' Cuts at: 3254 3961 4755 4775 3254 Size: 707 794 20 4924 Fragments arranged by size: 4924 794 707 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6445 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6410 DraI TTT'AAA Cuts at: 3749 3749 Size: 6445 DraIII CAC_nnn'GTG Cuts at: 2807 2807 Size: 6445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2915 5452 809 Size: 507 1599 2537 1802 Fragments arranged by size: 2537 1802 1599 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2535 3892 4781 Size: 54 1425 50 275 18 705 1357 889 Cuts at: 4781 6410 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1357 889 705 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3892 1537 Size: 275 2080 4090 Fragments arranged by size: 4090 2080 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 2283 2321 3485 3521 3545 4375 1353 Size: 756 174 38 1164 36 24 830 3423 Fragments arranged by size: 3423 1164 830 756 174 38 36 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2601 2827 3653 5032 5406 5552 Size: 1064 138 1012 226 826 1379 374 146 Cuts at: 5552 387 Size: 1280 Fragments arranged by size: 1379 1280 1064 1012 826 374 226 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2635 2714 2735 2755 2849 4508 5105 Size: 393 1003 79 21 20 94 1659 597 Cuts at: 5105 5892 6160 1239 Size: 787 268 1524 Fragments arranged by size: 1659 1524 1003 787 597 393 268 94 79 21 20 EcoO109I rG'GnC_Cy Cuts at: 2073 2084 3347 3348 3712 2073 Size: 11 1263 1 364 4806 Fragments arranged by size: 4806 1263 364 11 1 EcoRI G'AATT_C Cuts at: 3754 4132 3754 Size: 378 6067 FspI TGC'GCA Cuts at: 4951 4951 Size: 6445 HaeII r_GCGC'y Cuts at: 1678 3564 5592 1678 Size: 1886 2028 2531 Fragments arranged by size: 2531 2028 1886 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3012 3044 5086 5118 5160 Size: 32 135 32 1440 32 2042 32 42 Cuts at: 5160 5192 1373 Size: 32 2626 Fragments arranged by size: 2626 2042 1440 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3789 4035 669 Size: 988 281 1851 246 3079 Fragments arranged by size: 3079 1851 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6445 HpaI GTT'AAC Cuts at: 4035 4035 Size: 6445 KasI G'GCGC_C Cuts at: 3560 3560 Size: 6445 KpnI G_GTAC'C Cuts at: 3770 3770 Size: 6445 MfeI C'AATT_G Cuts at: 4044 4044 Size: 6445 MluI A'CGCG_T Cuts at: 6321 6321 Size: 6445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2126 2332 5559 5743 1305 Size: 821 206 3227 184 2007 Fragments arranged by size: 3227 2007 821 206 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4623 Fragments arranged by size: 4623 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2585 2865 3113 3425 3524 4403 4762 Size: 648 1419 280 248 312 99 879 359 Cuts at: 4762 4921 518 Size: 159 2042 Fragments arranged by size: 2042 1419 879 648 359 312 280 248 159 99 MspA1I CmG'CkG Cuts at: 1606 2269 2377 2506 3016 3669 3861 4538 5686 Size: 663 108 129 510 653 192 677 1148 Cuts at: 5686 5931 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 663 653 510 245 192 129 108 NarI GG'CG_CC Cuts at: 3561 3561 Size: 6445 NcoI C'CATG_G Cuts at: 513 1065 3378 513 Size: 552 2313 3580 Fragments arranged by size: 3580 2313 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6445 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6445 NotI GC'GGCC_GC Cuts at: 3892 3892 Size: 6445 NspI r_CATG'y Cuts at: 1132 1229 3424 3803 5348 1132 Size: 97 2195 379 1545 2229 Fragments arranged by size: 2229 2195 1545 379 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6445 PciI A'CATG_T Cuts at: 3420 5344 3420 Size: 1924 4521 PflMI CCAn_nnn'nTGG Cuts at: 1108 2401 2413 2686 1108 Size: 1293 12 273 4867 Fragments arranged by size: 4867 1293 273 12 PfoI T'CCnGG_A Cuts at: 2283 2754 2847 2283 Size: 471 93 5881 Fragments arranged by size: 5881 471 93 PmeI GTTT'AAAC Cuts at: 3749 3749 Size: 6445 PmlI CAC'GTG Cuts at: 2527 2527 Size: 6445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3380 3412 4514 4546 6027 6059 3380 Size: 32 1102 32 1481 32 3766 Fragments arranged by size: 3766 1481 1102 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2073 3712 2073 Size: 1639 4806 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6445 PsiI TTA'TAA Cuts at: 4015 4015 Size: 6445 PspOMI G'GGCC_C Cuts at: 3347 3347 Size: 6445 PstI C_TGCA'G Cuts at: 830 2985 3164 3618 3689 3797 830 Size: 2155 179 454 71 108 3478 Fragments arranged by size: 3478 2155 454 179 108 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6445 PvuII CAG'CTG Cuts at: 2269 2377 2269 Size: 108 6337 SacII CC_GC'GG Cuts at: 3670 3670 Size: 6445 SalI G'TCGA_C Cuts at: 1936 3787 1936 Size: 1851 4594 SanDI GG'GwC_CC Cuts at: 2073 3712 2073 Size: 1639 4806 SapI GCTCTTCn'nnn_ Cuts at: 3485 3485 Size: 6445 SbfI CC_TGCA'GG Cuts at: 3797 3797 Size: 6445 ScaI AGT'ACT Cuts at: 4693 6405 4693 Size: 1712 4733 SfcI C'TryA_G Cuts at: 826 1045 2981 3160 3614 3685 3793 4928 5609 Size: 219 1936 179 454 71 108 1135 681 Cuts at: 5609 5800 826 Size: 191 1471 Fragments arranged by size: 1936 1471 1135 681 454 219 191 179 108 71 SfoI GGC'GCC Cuts at: 3562 3562 Size: 6445 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 SgrAI Cr'CCGG_yG Cuts at: 1700 3557 1700 Size: 1857 4588 SmaI CCC'GGG Cuts at: 1872 2065 3772 1872 Size: 193 1707 4545 Fragments arranged by size: 4545 1707 193 SmlI C'TyrA_G Cuts at: 820 1017 1943 4549 5450 5712 820 Size: 197 926 2606 901 262 1553 Fragments arranged by size: 2606 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4236 493 Size: 3743 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6445 SphI G_CATG'C Cuts at: 1132 3803 1132 Size: 2671 3774 SspI AAT'ATT Cuts at: 5 52 4369 5 Size: 47 4317 2081 Fragments arranged by size: 4317 2081 47 StuI AGG'CCT Cuts at: 1517 3620 1517 Size: 2103 4342 StyI C'CwwG_G Cuts at: 513 1065 2178 2471 3378 3884 513 Size: 552 1113 293 907 506 3074 Fragments arranged by size: 3074 1113 907 552 506 293 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2054 3187 4472 4489 4642 4827 5272 Size: 1255 474 1133 1285 17 153 185 445 Cuts at: 5272 325 Size: 1498 Fragments arranged by size: 1498 1285 1255 1133 474 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4691 6403 96 Size: 274 80 33 51 1443 2714 1712 138 Fragments arranged by size: 2714 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6405 6437 6405 Size: 32 6413 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6445 XbaI T'CTAG_A Cuts at: 3781 3781 Size: 6445 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3259 3259 Size: 6445 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6445 XmaI C'CCGG_G Cuts at: 1870 2063 3770 1870 Size: 193 1707 4545 Fragments arranged by size: 4545 1707 193 XmnI GAAnn'nnTTC Cuts at: 2124 4574 2124 Size: 2450 3995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3157 4253 276 Size: 53 83 186 936 1623 1096 2468 Fragments arranged by size: 2468 1623 1096 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI