(Linear) (Six-base) MAPSORT of: KIEE4529.seq Check: 6014 from: 1 to: 1740 KIEE4529.orf With 182 enzymes: SgfI * July 3, 2012 19:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 931 1740 Size: 931 809 AatII G_ACGT'C Cuts at: 0 1154 1740 Size: 1154 586 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 730 750 1740 Size: 730 20 990 Fragments arranged by size: 990 730 20 AflIII A'CryG_T Cuts at: 0 422 1415 1740 Size: 422 993 325 Fragments arranged by size: 993 422 325 AleI CACnn'nnGTG Cuts at: 0 860 1740 Size: 860 880 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 214 1740 Size: 214 1526 AlwNI CAG_nnn'CTG Cuts at: 0 408 1740 Size: 408 1332 ApaI G_GGCC'C Cuts at: 0 1346 1740 Size: 1346 394 ApoI r'AATT_y Cuts at: 0 670 1045 1740 Size: 670 375 695 Fragments arranged by size: 695 670 375 AvaI C'yCGr_G Cuts at: 0 58 592 1098 1740 Size: 58 534 506 642 Fragments arranged by size: 642 534 506 58 BamHI G'GATC_C Cuts at: 0 585 809 1740 Size: 585 224 931 Fragments arranged by size: 931 585 224 BanI G'GyrC_C Cuts at: 0 1102 1171 1555 1740 Size: 1102 69 384 185 Fragments arranged by size: 1102 384 185 69 BanII G_rGCy'C Cuts at: 0 254 1346 1740 Size: 254 1092 394 Fragments arranged by size: 1092 394 254 BbeI G_GCGC'C Cuts at: 0 1559 1740 Size: 1559 181 BbsI GAAGACnn'nnnn_ Cuts at: 0 122 718 858 1740 Size: 122 596 140 882 Fragments arranged by size: 882 596 140 122 BclI T'GATC_A Cuts at: 0 776 1740 Size: 776 964 BglI GCCn_nnn'nGGC Cuts at: 0 1441 1740 Size: 1441 299 BlpI GC'TnA_GC Cuts at: 0 1528 1740 Size: 1528 212 Bme1580I G_kGCm'C Cuts at: 0 1105 1174 1346 1740 Size: 1105 69 172 394 Fragments arranged by size: 1105 394 172 69 BmgBI CAC'GTC Cuts at: 0 423 906 1740 Size: 423 483 834 Fragments arranged by size: 834 483 423 BmrI ACTGGGnnnn_n' Cuts at: 0 439 854 1740 Size: 439 415 886 Fragments arranged by size: 886 439 415 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 630 709 844 1740 Size: 630 79 135 896 Fragments arranged by size: 896 630 135 79 Bpu10I CC'TnA_GC Cuts at: 0 480 1740 Size: 480 1260 BsaI GGTCTCn'nnnn_ Cuts at: 0 25 820 1740 Size: 25 795 920 Fragments arranged by size: 920 795 25 BsaAI yAC'GTr Cuts at: 0 522 1740 Size: 522 1218 BsaBI GATnn'nnATC Cuts at: 0 814 1120 1740 Size: 814 306 620 Fragments arranged by size: 814 620 306 BsaHI Gr'CG_yC Cuts at: 0 1151 1556 1740 Size: 1151 405 184 Fragments arranged by size: 1151 405 184 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 358 388 1480 1510 1740 Size: 358 30 1092 30 230 Fragments arranged by size: 1092 358 230 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 931 1612 1732 1740 Size: 931 681 120 8 Fragments arranged by size: 931 681 120 8 BseYI C'CCAG_C Cuts at: 0 318 613 1267 1338 1481 1740 Size: 318 295 654 71 143 259 Fragments arranged by size: 654 318 295 259 143 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 1740 Size: 253 1487 BsiHKAI G_wGCw'C Cuts at: 0 1091 1529 1740 Size: 1091 438 211 Fragments arranged by size: 1091 438 211 BsmI GAATG_Cn' Cuts at: 0 1722 1740 Size: 1722 18 BsmBI CGTCTCn'nnnn_ Cuts at: 0 136 1740 Size: 136 1604 Bsp1286I G_dGCh'C Cuts at: 0 254 1091 1105 1174 1346 1529 1740 Size: 254 837 14 69 172 183 211 Fragments arranged by size: 837 254 211 183 172 69 14 BspHI T'CATG_A Cuts at: 0 146 1109 1740 Size: 146 963 631 Fragments arranged by size: 963 631 146 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 931 1740 Size: 931 809 BsrBI CCG'CTC Cuts at: 0 39 337 597 953 1038 1364 1670 1740 Size: 39 298 260 356 85 326 306 70 Fragments arranged by size: 356 326 306 298 260 85 70 39 BsrFI r'CCGG_y Cuts at: 0 87 434 1552 1740 Size: 87 347 1118 188 Fragments arranged by size: 1118 347 188 87 BstAPI GCAn_nnn'nTGC Cuts at: 0 188 1728 1740 Size: 188 1540 12 Fragments arranged by size: 1540 188 12 BstEII G'GTnAC_C Cuts at: 0 460 1740 Size: 460 1280 BstXI CCAn_nnnn'nTGG Cuts at: 0 822 1137 1740 Size: 822 315 603 Fragments arranged by size: 822 603 315 BstYI r'GATC_y Cuts at: 0 215 585 746 809 1740 Size: 215 370 161 63 931 Fragments arranged by size: 931 370 215 161 63 BtgI C'CryG_G Cuts at: 0 450 1373 1662 1740 Size: 450 923 289 78 Fragments arranged by size: 923 450 289 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 279 1097 1227 1524 1605 1740 Size: 279 818 130 297 81 135 Fragments arranged by size: 818 297 279 135 130 81 BtsI GCAGTG_nn' Cuts at: 0 1249 1740 Size: 1249 491 DraIII CAC_nnn'GTG Cuts at: 0 802 1740 Size: 802 938 DrdI GACnn_nn'nnGTC Cuts at: 0 910 1740 Size: 910 830 EaeI y'GGCC_r Cuts at: 0 530 1740 Size: 530 1210 EarI CTCTTCn'nnn_ Cuts at: 0 104 278 316 1480 1516 1540 1740 Size: 104 174 38 1164 36 24 200 Fragments arranged by size: 1164 200 174 104 38 36 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 596 822 1648 1740 Size: 596 226 826 92 Fragments arranged by size: 826 596 226 92 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 630 709 730 750 844 1740 Size: 630 79 21 20 94 896 Fragments arranged by size: 896 630 94 79 21 20 EcoO109I rG'GnC_Cy Cuts at: 0 68 79 1342 1343 1707 1740 Size: 68 11 1263 1 364 33 Fragments arranged by size: 1263 364 68 33 11 1 HaeII r_GCGC'y Cuts at: 0 1559 1740 Size: 1559 181 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1007 1039 1740 Size: 1007 32 701 Fragments arranged by size: 1007 701 32 KasI G'GCGC_C Cuts at: 0 1555 1740 Size: 1555 185 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 121 327 1740 Size: 121 206 1413 Fragments arranged by size: 1413 206 121 MslI CAynn'nnrTG Cuts at: 0 580 860 1108 1420 1519 1740 Size: 580 280 248 312 99 221 Fragments arranged by size: 580 312 280 248 221 99 MspA1I CmG'CkG Cuts at: 0 264 372 501 1011 1664 1740 Size: 264 108 129 510 653 76 Fragments arranged by size: 653 510 264 129 108 76 NarI GG'CG_CC Cuts at: 0 1556 1740 Size: 1556 184 NcoI C'CATG_G Cuts at: 0 1373 1740 Size: 1373 367 NspI r_CATG'y Cuts at: 0 1419 1740 Size: 1419 321 PciI A'CATG_T Cuts at: 0 1415 1740 Size: 1415 325 PflMI CCAn_nnn'nTGG Cuts at: 0 396 408 681 1740 Size: 396 12 273 1059 Fragments arranged by size: 1059 396 273 12 PfoI T'CCnGG_A Cuts at: 0 278 749 842 1740 Size: 278 471 93 898 Fragments arranged by size: 898 471 278 93 PmlI CAC'GTG Cuts at: 0 522 1740 Size: 522 1218 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1375 1407 1740 Size: 1375 32 333 Fragments arranged by size: 1375 333 32 PpuMI rG'GwC_Cy Cuts at: 0 68 1707 1740 Size: 68 1639 33 Fragments arranged by size: 1639 68 33 PspOMI G'GGCC_C Cuts at: 0 1342 1740 Size: 1342 398 PstI C_TGCA'G Cuts at: 0 980 1159 1613 1684 1740 Size: 980 179 454 71 56 Fragments arranged by size: 980 454 179 71 56 PvuII CAG'CTG Cuts at: 0 264 372 1740 Size: 264 108 1368 Fragments arranged by size: 1368 264 108 SacII CC_GC'GG Cuts at: 0 1665 1740 Size: 1665 75 SanDI GG'GwC_CC Cuts at: 0 68 1707 1740 Size: 68 1639 33 Fragments arranged by size: 1639 68 33 SapI GCTCTTCn'nnn_ Cuts at: 0 1480 1740 Size: 1480 260 SfcI C'TryA_G Cuts at: 0 976 1155 1609 1680 1740 Size: 976 179 454 71 60 Fragments arranged by size: 976 454 179 71 60 SfoI GGC'GCC Cuts at: 0 1557 1740 Size: 1557 183 SgrAI Cr'CCGG_yG Cuts at: 0 1552 1740 Size: 1552 188 SmaI CCC'GGG Cuts at: 0 60 1740 Size: 60 1680 StuI AGG'CCT Cuts at: 0 1615 1740 Size: 1615 125 StyI C'CwwG_G Cuts at: 0 173 466 1373 1740 Size: 173 293 907 367 Fragments arranged by size: 907 367 293 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 49 1182 1740 Size: 49 1133 558 Fragments arranged by size: 1133 558 49 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1254 1740 Size: 1254 486 XmaI C'CCGG_G Cuts at: 0 58 1740 Size: 58 1682 XmnI GAAnn'nnTTC Cuts at: 0 119 1740 Size: 119 1621 ZraI GAC'GTC Cuts at: 0 1152 1740 Size: 1152 588 Enzymes that do cut: AarI AatII AcuI AflIII AleI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BclI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MslI MspA1I NarI NcoI NspI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacII SanDI SapI SfcI SfoI SgrAI SmaI StuI StyI TaqII XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BfrBI BglII BmtI BplI BpuEI BsaWI BsiEI BsiWI BspEI BsrDI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I ClaI CspCI DraI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI