(Circular) (Six-base) MAPSORT of: pFN21AE5304.seq Check: 3301 from: 1 to: 5503 pFN21AE5304 5503 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2388 2581 3313 278 Size: 53 83 186 936 852 193 732 2468 Fragments arranged by size: 2468 936 852 732 193 186 83 53 AccI GT'mk_AC Cuts at: 1937 2846 1937 Size: 909 4594 Acc65I G'GTAC_C Cuts at: 2824 2824 Size: 5503 AclI AA'CG_TT Cuts at: 1563 3630 4003 1563 Size: 2067 373 3063 Fragments arranged by size: 3063 2067 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3566 4950 5218 1632 Size: 1934 1384 268 1917 Fragments arranged by size: 1934 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2291 820 Size: 197 1274 4032 Fragments arranged by size: 4032 1274 197 AflIII A'CryG_T Cuts at: 1203 4402 5379 1203 Size: 3199 977 1327 Fragments arranged by size: 3199 1327 977 AgeI A'CCGG_T Cuts at: 4334 4334 Size: 5503 AhdI GACnn_n'nnGTC Cuts at: 2405 2582 4232 2405 Size: 177 1650 3676 Fragments arranged by size: 3676 1650 177 AleI CACnn'nnGTG Cuts at: 1166 2441 2621 2715 1166 Size: 1275 180 94 3954 Fragments arranged by size: 3954 1275 180 94 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5503 AlwNI CAG_nnn'CTG Cuts at: 2263 2344 3246 4818 2263 Size: 81 902 1572 2948 Fragments arranged by size: 2948 1572 902 81 ApaLI G'TGCA_C Cuts at: 3560 4716 3560 Size: 1156 4347 ApoI r'AATT_y Cuts at: 1493 2116 2812 3038 3190 5299 1493 Size: 623 696 226 152 2109 1697 Fragments arranged by size: 2109 1697 696 623 226 152 AseI AT'TA_AT Cuts at: 160 1032 4057 160 Size: 872 3025 1606 Fragments arranged by size: 3025 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5503 AvaI C'yCGr_G Cuts at: 1870 1943 2352 2828 1870 Size: 73 409 476 4545 Fragments arranged by size: 4545 476 409 73 BamHI G'GATC_C Cuts at: 2488 2833 3200 2488 Size: 345 367 4791 Fragments arranged by size: 4791 367 345 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2824 4279 618 Size: 325 217 627 1037 1455 1842 Fragments arranged by size: 1842 1455 1037 627 325 217 BanII G_rGCy'C Cuts at: 1391 2822 1391 Size: 1431 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2500 2600 2640 928 Size: 971 601 100 40 3791 Fragments arranged by size: 3791 971 601 100 40 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2590 2624 3694 3728 1903 Size: 34 50 34 569 34 1070 34 3678 Fragments arranged by size: 3678 1070 569 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3395 4605 3395 Size: 1210 4293 BclI T'GATC_A Cuts at: 1548 1548 Size: 5503 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4114 136 Size: 107 122 71 631 605 2442 1525 Fragments arranged by size: 2442 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5498 5498 Size: 5503 BlpI GC'TnA_GC Cuts at: 2460 2652 2920 2460 Size: 192 268 5043 Fragments arranged by size: 5043 268 192 Bme1580I G_kGCm'C Cuts at: 1790 3564 4720 1790 Size: 1774 1156 2573 Fragments arranged by size: 2573 1774 1156 BmgBI CAC'GTC Cuts at: 1318 2485 1318 Size: 1167 4336 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2582 2722 2807 Size: 416 53 83 399 24 1165 140 85 Cuts at: 2807 3234 4192 442 Size: 427 958 1753 Fragments arranged by size: 1753 1165 958 427 416 399 140 85 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5503 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2228 2228 Size: 5503 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4163 1239 Size: 2924 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3628 4493 4791 3628 Size: 865 298 4340 Fragments arranged by size: 4340 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2145 2392 4166 882 Size: 617 646 247 1774 2219 Fragments arranged by size: 2219 1774 646 617 247 BsaAI yAC'GTr Cuts at: 493 1204 3294 493 Size: 711 2090 2702 Fragments arranged by size: 2702 2090 711 BsaBI GATnn'nnATC Cuts at: 5497 5497 Size: 5503 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2385 2578 3310 3692 Size: 53 83 186 936 852 193 732 382 Cuts at: 3692 4327 275 Size: 635 1451 Fragments arranged by size: 1451 936 852 732 635 382 193 186 83 53 BsaWI w'CCGG_w Cuts at: 3936 4334 4608 4755 5166 3936 Size: 398 274 147 411 4273 Fragments arranged by size: 4273 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5278 Fragments arranged by size: 5278 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5503 BseYI C'CCAG_C Cuts at: 2050 4706 5202 2050 Size: 2656 496 2351 Fragments arranged by size: 2656 2351 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2584 1163 Size: 573 848 4082 Fragments arranged by size: 4082 848 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2953 3714 4742 1540 Size: 275 186 952 761 1028 2301 Fragments arranged by size: 2301 1028 952 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3564 3649 4720 3564 Size: 85 1071 4347 Fragments arranged by size: 4347 1071 85 BsmI GAATG_Cn' Cuts at: 3014 3107 3014 Size: 93 5410 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2822 3564 3649 4720 1391 Size: 399 1032 742 85 1071 2174 Fragments arranged by size: 2174 1071 1032 742 399 85 BspEI T'CCGG_A Cuts at: 5166 5166 Size: 5503 BspHI T'CATG_A Cuts at: 3392 5122 3392 Size: 1730 3773 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2606 2858 844 Size: 1762 252 3489 Fragments arranged by size: 3489 1762 252 BsrBI CCG'CTC Cuts at: 1394 1823 3390 1394 Size: 429 1567 3507 Fragments arranged by size: 3507 1567 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2728 3998 4172 65 Size: 1183 1480 1270 174 1396 Fragments arranged by size: 1480 1396 1270 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4147 4334 1700 Size: 110 2337 187 2869 Fragments arranged by size: 2869 2337 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5503 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5503 BssSI C'ACGA_G Cuts at: 3563 4575 3563 Size: 1012 4491 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5503 BstBI TT'CG_AA Cuts at: 4307 4307 Size: 5503 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5503 BstXI CCAn_nnnn'nTGG Cuts at: 2443 2500 2797 2443 Size: 57 297 5149 Fragments arranged by size: 5149 297 57 BstYI r'GATC_y Cuts at: 1972 2488 2700 2833 3200 3585 3602 5054 5140 Size: 516 212 133 367 385 17 1452 86 Cuts at: 5140 5152 5498 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 516 385 367 346 212 133 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4951 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4764 Fragments arranged by size: 4764 683 56 BtsI GCAGTG_nn' Cuts at: 3019 3813 3833 3019 Size: 794 20 4689 Fragments arranged by size: 4689 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5503 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5468 DraI TTT'AAA Cuts at: 2807 2807 Size: 5503 DraIII CAC_nnn'GTG Cuts at: 2485 2485 Size: 5503 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2577 4510 809 Size: 507 1261 1933 1802 Fragments arranged by size: 1933 1802 1261 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2950 3839 5468 Size: 54 1425 50 275 18 1120 889 1629 Cuts at: 5468 8 Size: 43 Fragments arranged by size: 1629 1425 1120 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2950 1537 Size: 275 1138 4090 Fragments arranged by size: 4090 1138 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2390 3433 1353 Size: 1037 1043 3423 Fragments arranged by size: 3423 1043 1037 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2693 4090 4464 4610 387 Size: 1064 138 1104 1397 374 146 1280 Fragments arranged by size: 1397 1280 1104 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3566 4163 4950 5218 1239 Size: 393 1934 597 787 268 1524 Fragments arranged by size: 1934 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2260 2539 2732 2260 Size: 279 193 5031 Fragments arranged by size: 5031 279 193 EcoRI G'AATT_C Cuts at: 2812 3190 2812 Size: 378 5125 FspI TGC'GCA Cuts at: 4009 4009 Size: 5503 HaeII r_GCGC'y Cuts at: 1678 2646 4650 1678 Size: 968 2004 2531 Fragments arranged by size: 2531 2004 968 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2121 2153 2391 2423 2568 Size: 32 135 32 549 32 238 32 145 Cuts at: 2568 2600 2692 2724 4144 4176 4218 4250 1373 Size: 32 92 32 1420 32 42 32 2626 Fragments arranged by size: 2626 1420 549 238 145 135 92 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2425 2847 3093 669 Size: 988 281 487 422 246 3079 Fragments arranged by size: 3079 988 487 422 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5503 HpaI GTT'AAC Cuts at: 2425 3093 2425 Size: 668 4835 KpnI G_GTAC'C Cuts at: 2828 2828 Size: 5503 MfeI C'AATT_G Cuts at: 3102 3102 Size: 5503 MluI A'CGCG_T Cuts at: 5379 5379 Size: 5503 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4617 4801 1305 Size: 3312 184 2007 Fragments arranged by size: 3312 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3681 Fragments arranged by size: 3681 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2441 2621 2715 3461 3820 3979 518 Size: 648 1275 180 94 746 359 159 2042 Fragments arranged by size: 2042 1275 746 648 359 180 159 94 MspA1I CmG'CkG Cuts at: 1606 2054 2197 2287 2464 2919 3596 4744 4989 Size: 448 143 90 177 455 677 1148 245 Cuts at: 4989 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 455 448 245 177 143 90 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4951 NdeI CA'TA_TG Cuts at: 387 387 Size: 5503 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5503 NotI GC'GGCC_GC Cuts at: 2950 2950 Size: 5503 NspI r_CATG'y Cuts at: 1132 1229 2861 4406 1132 Size: 97 1632 1545 2229 Fragments arranged by size: 2229 1632 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5503 PciI A'CATG_T Cuts at: 4402 4402 Size: 5503 PflMI CCAn_nnn'nTGG Cuts at: 1108 2263 1108 Size: 1155 4348 PmeI GTTT'AAAC Cuts at: 2807 2807 Size: 5503 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3572 3604 5085 5117 3572 Size: 32 1481 32 3958 Fragments arranged by size: 3958 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2539 2732 2539 Size: 193 5310 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5503 PsiI TTA'TAA Cuts at: 3073 3073 Size: 5503 PstI C_TGCA'G Cuts at: 830 2855 830 Size: 2025 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5503 PvuII CAG'CTG Cuts at: 2054 2287 2464 2054 Size: 233 177 5093 Fragments arranged by size: 5093 233 177 SalI G'TCGA_C Cuts at: 1936 2845 1936 Size: 909 4594 SanDI GG'GwC_CC Cuts at: 2539 2539 Size: 5503 SbfI CC_TGCA'GG Cuts at: 2855 2855 Size: 5503 ScaI AGT'ACT Cuts at: 3751 5463 3751 Size: 1712 3791 SfcI C'TryA_G Cuts at: 826 1045 2851 3986 4667 4858 826 Size: 219 1806 1135 681 191 1471 Fragments arranged by size: 1806 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5503 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5503 SmaI CCC'GGG Cuts at: 1872 2354 2830 1872 Size: 482 476 4545 Fragments arranged by size: 4545 482 476 SmlI C'TyrA_G Cuts at: 820 1017 1943 2291 3607 4508 4770 820 Size: 197 926 348 1316 901 262 1553 Fragments arranged by size: 1553 1316 926 901 348 262 197 SnaBI TAC'GTA Cuts at: 493 3294 493 Size: 2801 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5503 SphI G_CATG'C Cuts at: 1132 2861 1132 Size: 1729 3774 SrfI GCCC'GGGC Cuts at: 2354 2354 Size: 5503 SspI AAT'ATT Cuts at: 5 52 3427 5 Size: 47 3375 2081 Fragments arranged by size: 3375 2081 47 StuI AGG'CCT Cuts at: 1517 2110 1517 Size: 593 4910 StyI C'CwwG_G Cuts at: 513 1065 2025 2439 2942 513 Size: 552 960 414 503 3074 Fragments arranged by size: 3074 960 552 503 414 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2270 2789 3530 3547 3700 3885 4330 Size: 1255 690 519 741 17 153 185 445 Cuts at: 4330 325 Size: 1498 Fragments arranged by size: 1498 1255 741 690 519 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2307 3749 5461 Size: 274 80 33 51 1443 330 1442 1712 Cuts at: 5461 96 Size: 138 Fragments arranged by size: 1712 1443 1442 330 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2022 2054 5463 5495 2022 Size: 32 3409 32 2030 Fragments arranged by size: 3409 2030 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5503 XbaI T'CTAG_A Cuts at: 2839 2839 Size: 5503 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5503 XmaI C'CCGG_G Cuts at: 1870 2352 2828 1870 Size: 482 476 4545 Fragments arranged by size: 4545 482 476 XmnI GAAnn'nnTTC Cuts at: 3632 3632 Size: 5503 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2386 2579 3311 276 Size: 53 83 186 936 852 193 732 2468 Fragments arranged by size: 2468 936 852 732 193 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SwaI XcmI