################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BanI BbeI BcgI BclI BcnI BfrI BglI BglII BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HapII HgaI Hin1I HpaII KasI KpnI Ksp632I KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 669 GTCTAC GT^[AC][GT]AC AhdI 79 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 431 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 79 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 41 CTCGGG C^[CT]CG[GA]G BamHI 661 GGATCC G^GATCC BanII 66 GAGCTC G[GA]GC[CT]^C BbrPI 969 CACGTG CAC^GTG BciVI 117 GTATCC GTATCC(6/5) BpmI 961 CTCCAG CTGGAG(16/14) BsaI 1282 GAGACC GGTCTC(1/5) BsgI 1256 GTGCAG GTGCAG(16/14) BsmI 1239 GAATGC GAATGC(1/-1) BsoBI 41 CTCGGG C^[CT]CG[GA]G BspMI 53 ACCTGC ACCTGC(4/8) BsrDI 1073 GCAATG GCAATG(2/0) BssSI 511 CTCGTG CACGAG(-5/-1) BstYI 661 GGATCC [GA]^GATC[CT] CfoI 329 GCGC GCG^C DraI 1087 TTTAAA TTT^AAA DraII 1206 AGGTCCC [GA]G^G[ACGT]CC[CT] DsaI 176 CCGTGG C^C[GA][CT]GG Eam1105I 79 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 1206 AGGTCCC [GA]G^G[ACGT]CC[CT] FauI 941 CCCGC CCCGC(4/6) HaeII 328 GGCGCT [GA]GCGC^[CT] HhaI 329 GCGC GCG^C HinP1I 329 GCGC G^CGC HindIII 676 AAGCTT A^AGCTT HpaI 1177 GTTAAC GTT^AAC MfeI 935 CAATTG C^AATTG MflI 661 GGATCC [GA]^GATC[CT] MunI 935 CAATTG C^AATTG PmaCI 969 CACGTG CAC^GTG PmlI 969 CACGTG CAC^GTG PpuMI 1206 AGGTCCC [GA]G^G[AT]CC[CT] PstI 1132 CTGCAG CTGCA^G SacI 66 GAGCTC GAGCT^C SfcI 1132 CTGCAG C^T[GA][CT]AG XhoII 661 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 781 GAATTT [GA]^AATT[CT] 818 AAATTC ApoI 781 GAATTT [GA]^AATT[CT] 818 AAATTC AspI 453 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1227 GACTGAGTC BalI 179 TGGCCA TGG^CCA 389 TGGCCA BbsI 162 GAAGAC GAAGAC(2/6) 450 GAAGAC BbvCI 89 CCTCAGC CCTCAGC(-5/-2) 1219 CCTCAGC BlpI 724 GCTAAGC GC^T[ACGT]AGC 907 GCTGAGC Bpu1102I 724 GCTAAGC GC^T[ACGT]AGC 907 GCTGAGC BpuAI 162 GAAGAC GAAGAC(2/6) 450 GAAGAC BseRI 1108 CTCCTC GAGGAG(10/8) 271 GAGGAG BsmFI 897 GTCCC GGGAC(10/14) 1208 GTCCC BssKI 788 CCAGG ^CC[ACGT]GG 1278 CCAGG BstAPI 4 GCATCCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 343 GCAAAAGATGC BstNI 788 CCAGG CC^[AT]GG 1278 CCAGG BstXI 455 CCAAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1028 CCAAGCACATGG CelII 724 GCTAAGC GC^T[ACGT]AGC 907 GCTGAGC EaeI 179 TGGCCA [CT]^GGCC[GA] 389 TGGCCA EcoRII 788 CCAGG ^CC[AT]GG 1278 CCAGG HincII 49 GTCAAC GT[CT]^[GA]AC 1177 GTTAAC HindII 49 GTCAAC GT[CT]^[GA]AC 1177 GTTAAC MluNI 179 TGGCCA TGG^CCA 389 TGGCCA MscI 179 TGGCCA TGG^CCA 389 TGGCCA MspA1I 1129 CAGCTG C[AC]G^C[GT]G 1222 CAGCTG MvaI 788 CCAGG CC^[AT]GG 1278 CCAGG PflFI 453 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1227 GACTGAGTC PvuII 1129 CAGCTG CAG^CTG 1222 CAGCTG ScrFI 788 CCAGG CC^[ACGT]GG 1278 CCAGG Tth111I 453 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1227 GACTGAGTC XcmI 167 CCATCACCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 377 CCAAGGATTCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BbvI BfaI BmyI BsaAI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BstF5I Cac8I Cfr13I DdeI DpnII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI