(Linear) (Six-base) MAPSORT of: KIEE4421.seq Check: 8204 from: 1 to: 1311 KIEE4421.orf With 182 enzymes: SgfI * March 22, 2012 17:58 .. AccI GT'mk_AC Cuts at: 0 670 1311 Size: 670 641 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 148 1311 Size: 148 1163 AhdI GACnn_n'nnGTC Cuts at: 0 84 1311 Size: 84 1227 AleI CACnn'nnGTG Cuts at: 0 175 406 686 1311 Size: 175 231 280 625 Fragments arranged by size: 625 280 231 175 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 554 586 1311 Size: 554 32 725 Fragments arranged by size: 725 554 32 AlwNI CAG_nnn'CTG Cuts at: 0 436 1311 Size: 436 875 ApoI r'AATT_y Cuts at: 0 781 818 1311 Size: 781 37 493 Fragments arranged by size: 781 493 37 AvaI C'yCGr_G Cuts at: 0 41 1311 Size: 41 1270 BamHI G'GATC_C Cuts at: 0 661 1311 Size: 661 650 BanII G_rGCy'C Cuts at: 0 70 1311 Size: 70 1241 BbsI GAAGACnn'nnnn_ Cuts at: 0 169 457 1311 Size: 169 288 854 Fragments arranged by size: 854 288 169 BbvCI CC'TCA_GC Cuts at: 0 90 1220 1311 Size: 90 1130 91 Fragments arranged by size: 1130 91 90 BciVI GTATCCnnnnn_n' Cuts at: 0 128 1311 Size: 128 1183 BlpI GC'TnA_GC Cuts at: 0 725 908 1311 Size: 725 183 403 Fragments arranged by size: 725 403 183 BmgBI CAC'GTC Cuts at: 0 1161 1311 Size: 1161 150 BmrI ACTGGGnnnn_n' Cuts at: 0 347 380 667 1311 Size: 347 33 287 644 Fragments arranged by size: 644 347 287 33 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 946 1311 Size: 946 365 Bpu10I CC'TnA_GC Cuts at: 0 90 830 1220 1244 1311 Size: 90 740 390 24 67 Fragments arranged by size: 740 390 90 67 24 BsaI GGTCTCn'nnnn_ Cuts at: 0 1276 1311 Size: 1276 35 BsaAI yAC'GTr Cuts at: 0 74 925 971 1311 Size: 74 851 46 340 Fragments arranged by size: 851 340 74 46 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 554 584 1311 Size: 554 30 727 Fragments arranged by size: 727 554 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 286 1099 1311 Size: 286 813 212 Fragments arranged by size: 813 286 212 BseYI C'CCAG_C Cuts at: 0 1286 1311 Size: 1286 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1277 1311 Size: 1277 34 BsiHKAI G_wGCw'C Cuts at: 0 70 195 914 1311 Size: 70 125 719 397 Fragments arranged by size: 719 397 125 70 BsmI GAATG_Cn' Cuts at: 0 1245 1311 Size: 1245 66 Bsp1286I G_dGCh'C Cuts at: 0 70 195 914 1311 Size: 70 125 719 397 Fragments arranged by size: 719 397 125 70 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 62 1311 Size: 62 1249 BsrDI GCAATG_nn' Cuts at: 0 1080 1311 Size: 1080 231 BssSI C'ACGA_G Cuts at: 0 511 1311 Size: 511 800 BstAPI GCAn_nnn'nTGC Cuts at: 0 10 349 1311 Size: 10 339 962 Fragments arranged by size: 962 339 10 BstXI CCAn_nnnn'nTGG Cuts at: 0 462 1035 1311 Size: 462 573 276 Fragments arranged by size: 573 462 276 BstYI r'GATC_y Cuts at: 0 661 1311 Size: 661 650 BtgI C'CryG_G Cuts at: 0 176 1311 Size: 176 1135 BtsI GCAGTG_nn' Cuts at: 0 437 447 525 1311 Size: 437 10 78 786 Fragments arranged by size: 786 437 78 10 DraI TTT'AAA Cuts at: 0 1089 1311 Size: 1089 222 EaeI y'GGCC_r Cuts at: 0 179 389 1311 Size: 179 210 922 Fragments arranged by size: 922 210 179 EcoICRI GAG'CTC Cuts at: 0 68 1311 Size: 68 1243 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 148 946 1311 Size: 148 798 365 Fragments arranged by size: 798 365 148 EcoO109I rG'GnC_Cy Cuts at: 0 1207 1311 Size: 1207 104 HaeII r_GCGC'y Cuts at: 0 332 1311 Size: 332 979 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 70 102 177 209 735 767 1045 1077 Size: 70 32 75 32 526 32 278 32 Cuts at: 1077 1155 1187 1218 1250 1311 Size: 78 32 31 32 61 Fragments arranged by size: 526 278 78 75 70 61 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 0 51 1179 1311 Size: 51 1128 132 Fragments arranged by size: 1128 132 51 HindIII A'AGCT_T Cuts at: 0 676 1311 Size: 676 635 HpaI GTT'AAC Cuts at: 0 1179 1311 Size: 1179 132 MfeI C'AATT_G Cuts at: 0 935 1311 Size: 935 376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 478 1013 1311 Size: 478 535 298 Fragments arranged by size: 535 478 298 MscI TGG'CCA Cuts at: 0 181 391 1311 Size: 181 210 920 Fragments arranged by size: 920 210 181 MslI CAynn'nnrTG Cuts at: 0 175 406 686 1039 1311 Size: 175 231 280 353 272 Fragments arranged by size: 353 280 272 231 175 MspA1I CmG'CkG Cuts at: 0 1131 1224 1311 Size: 1131 93 87 Fragments arranged by size: 1131 93 87 PmlI CAC'GTG Cuts at: 0 971 1311 Size: 971 340 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 554 586 1311 Size: 554 32 725 Fragments arranged by size: 725 554 32 PpuMI rG'GwC_Cy Cuts at: 0 1207 1311 Size: 1207 104 PstI C_TGCA'G Cuts at: 0 1136 1311 Size: 1136 175 PvuII CAG'CTG Cuts at: 0 1131 1224 1311 Size: 1131 93 87 Fragments arranged by size: 1131 93 87 SacI G_AGCT'C Cuts at: 0 70 1311 Size: 70 1241 SfcI C'TryA_G Cuts at: 0 1132 1311 Size: 1132 179 StyI C'CwwG_G Cuts at: 0 59 377 996 1251 1311 Size: 59 318 619 255 60 Fragments arranged by size: 619 318 255 60 59 TatI w'GTAC_w Cuts at: 0 103 1311 Size: 103 1208 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 998 1030 1311 Size: 998 32 281 Fragments arranged by size: 998 281 32 Tth111I GACn'n_nGTC Cuts at: 0 456 1230 1311 Size: 456 774 81 Fragments arranged by size: 774 456 81 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 174 384 1311 Size: 174 210 927 Fragments arranged by size: 927 210 174 Enzymes that do cut: AccI AcuI AhdI AleI AloI AlwNI ApoI AvaI BamHI BanII BbsI BbvCI BciVI BlpI BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BstAPI BstXI BstYI BtgI BtsI DraI EaeI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I PmlI PpiI PpuMI PstI PvuII SacI SfcI StyI TatI TstI Tth111I XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI BcgI BclI BfrBI BglI BglII Bme1580I BmtI BplI BpuEI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII XbaI XhoI XmaI XmnI ZraI