################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AccIII AclI AflII AgeI AocI AscI AseI AsnI Asp700I AspI AvaI AviII AvrII BbrPI BciVI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII BstAPI BstBI Bsu36I Cfr10I ClaI CpoI DraI DraIII EagI EclXI Eco52I Eco81I EcoNI EcoRV EcoT22I FseI FspI HindIII KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SbfI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 25 GACGTC GACGT^C Acc65I 1144 GGTACC G^GTACC AccII 1257 CGCG CG^CG AflIII 914 ACATGT A^C[GA][CT]GT AhdI 2104 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 616 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 887 AGCGCT AGC^GCT ApaI 1010 GGGCCC GGGCC^C Asp718I 1144 GGTACC G^GTACC AspEI 2104 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 158 GGCGCC GGCGC^C BbsI 1860 GTCTTC GAAGAC(2/6) BcgI 1314 CGATGAATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 767 TGATCA T^GATCA BlpI 1965 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1965 GCTAAGC GC^T[ACGT]AGC BpuAI 1860 GTCTTC GAAGAC(2/6) BsaI 1180 GAGACC GGTCTC(1/5) BsgI 667 CTGCAC GTGCAG(16/14) BsmI 1395 GAATGC GAATGC(1/-1) BspHI 950 TCATGA T^CATGA BspLU11I 914 ACATGT A^CATGT BspMI 671 ACCTGC ACCTGC(4/8) BsrBI 1066 GAGCGG CCGCTC(-3/-3) BsrDI 401 GCAATG GCAATG(2/0) Bst1107I 1200 GTATAC GTA^TAC BstEII 370 GGTGACC G^GT[ACGT]ACC BstPI 370 GGTGACC G^GT[ACGT]ACC BstUI 1257 CGCG CG^CG BstXI 510 CCAGGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1200 GTATAC GTA^TAC CelII 1965 GCTAAGC GC^T[ACGT]AGC DrdI 373 GACCAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2104 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 887 AGCGCT AGC^GCT EcoO65I 370 GGTGACC G^GT[ACGT]ACC EcoRI 445 GAATTC G^AATTC FbaI 767 TGATCA T^GATCA HpaI 141 GTTAAC GTT^AAC KasI 158 GGCGCC G^GCGCC KpnI 1144 GGTACC GGTAC^C MvnI 1257 CGCG CG^CG NarI 158 GGCGCC GG^CGCC NcoI 177 CCATGG C^CATGG PpuMI 299 AGGACCT [GA]G^G[AT]CC[CT] PstI 613 CTGCAG CTGCA^G PvuII 349 CAGCTG CAG^CTG RcaI 950 TCATGA T^CATGA SacI 2010 GAGCTC GAGCT^C SalI 62 GTCGAC G^TCGAC SapI 2012 GCTCTTC GCTCTTC(1/4) SfcI 613 CTGCAG C^T[GA][CT]AG SfiI 1566 GGCCAAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 158 GGCGCC GGC^GCC StuI 536 AGGCCT AGG^CCT TfiI 235 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 337 AAATTT [GA]^AATT[CT] 445 GAATTC AcyI 25 GACGTC G[GA]^CG[CT]C 158 GGCGCC Alw44I 414 GTGCAC G^TGCAC 1099 GTGCAC ApaLI 414 GTGCAC G^TGCAC 1099 GTGCAC ApoI 337 AAATTT [GA]^AATT[CT] 445 GAATTC BalI 204 TGGCCA TGG^CCA 1574 TGGCCA BanI 158 GGCGCC G^G[CT][GA]CC 1144 GGTACC BbvCI 396 CCTCAGC CCTCAGC(-5/-2) 1798 GCTGAGG BglI 77 GCCGTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1567 GCCAAGATGGC BglII 1118 AGATCT A^GATCT 1748 AGATCT BsaHI 25 GACGTC G[GA]^CG[CT]C 158 GGCGCC BssSI 629 CTCGTG CACGAG(-5/-1) 1139 CTCGTG DraII 299 AGGACCT [GA]G^G[ACGT]CC[CT] 1009 GGGGCCC DsaI 177 CCATGG C^C[GA][CT]GG 581 CCACGG EaeI 204 TGGCCA [CT]^GGCC[GA] 1574 TGGCCA EcoO109I 299 AGGACCT [GA]G^G[ACGT]CC[CT] 1009 GGGGCCC FauI 1059 CCCGC CCCGC(4/6) 1068 GCGGG Hin1I 25 GACGTC G[GA]^CG[CT]C 158 GGCGCC MluNI 204 TGGCCA TGG^CCA 1574 TGGCCA MscI 204 TGGCCA TGG^CCA 1574 TGGCCA MseI 142 TTAA T^TAA 185 TTAA MslI 480 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 670 CACCTGCATG MspA1I 349 CAGCTG C[AC]G^C[GT]G 1060 CCGCTG NspI 914 ACATGT [GA]CATG^[CT] 1661 ACATGC ScaI 714 AGTACT AGT^ACT 2083 AGTACT SmlI 283 CTTGAG C^T[CT][GA]AG 1956 CTCAAG TaqI 63 TCGA T^CGA 1112 TCGA Tru9I 142 TTAA T^TAA 185 TTAA TthHB8I 63 TCGA T^CGA 1112 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AspHI AvaII BamHI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I Tsp509I TspRI XhoII