(Circular) (Six-base) MAPSORT of: pFN21AE3582.seq Check: 9820 from: 1 to: 6379 pFN21AE3582 6379 bp With 182 enzymes: SgfI * January 27, 2015 16:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2460 4189 278 Size: 53 83 186 936 924 1729 2468 Fragments arranged by size: 2468 1729 936 924 186 83 53 AccI GT'mk_AC Cuts at: 1937 2343 2744 3722 1937 Size: 406 401 978 4594 Fragments arranged by size: 4594 978 406 401 Acc65I G'GTAC_C Cuts at: 2052 3700 2052 Size: 1648 4731 AclI AA'CG_TT Cuts at: 1563 4506 4879 1563 Size: 2943 373 3063 Fragments arranged by size: 3063 2943 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2107 2514 2529 4442 5826 6094 1632 Size: 475 407 15 1913 1384 268 1917 Fragments arranged by size: 1917 1913 1384 475 407 268 15 AflII C'TTAA_G Cuts at: 820 1017 2516 820 Size: 197 1499 4683 Fragments arranged by size: 4683 1499 197 AflIII A'CryG_T Cuts at: 1203 2790 5278 6255 1203 Size: 1587 2488 977 1327 Fragments arranged by size: 2488 1587 1327 977 AgeI A'CCGG_T Cuts at: 2319 5210 2319 Size: 2891 3488 AhdI GACnn_n'nnGTC Cuts at: 3076 5108 3076 Size: 2032 4347 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6379 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2751 3063 1184 Size: 1567 312 4500 Fragments arranged by size: 4500 1567 312 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2015 2047 2240 2272 2594 2626 2015 Size: 32 193 32 322 32 5768 Fragments arranged by size: 5768 322 193 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2041 3037 4122 5694 2041 Size: 996 1085 1572 2726 Fragments arranged by size: 2726 1572 1085 996 ApaLI G'TGCA_C Cuts at: 4436 5592 4436 Size: 1156 5223 ApoI r'AATT_y Cuts at: 1493 3688 3914 4066 6175 1493 Size: 2195 226 152 2109 1697 Fragments arranged by size: 2195 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4933 160 Size: 872 3901 1606 Fragments arranged by size: 3901 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 AvaI C'yCGr_G Cuts at: 1870 1943 2019 2244 2606 2896 3704 1870 Size: 73 76 225 362 290 808 4545 Fragments arranged by size: 4545 808 362 290 225 76 73 BamHI G'GATC_C Cuts at: 2707 3709 4076 2707 Size: 1002 367 5010 Fragments arranged by size: 5010 1002 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2052 3197 3700 5155 618 Size: 325 217 627 265 1145 503 1455 1842 Fragments arranged by size: 1842 1455 1145 627 503 325 265 217 BanII G_rGCy'C Cuts at: 1391 2905 3351 3639 3698 1391 Size: 1514 446 288 59 4072 Fragments arranged by size: 4072 1514 446 288 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5408 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4570 4604 1903 Size: 34 2633 34 3678 Fragments arranged by size: 3678 2633 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4271 5481 4271 Size: 1210 5169 BclI T'GATC_A Cuts at: 1548 1548 Size: 6379 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4990 136 Size: 107 122 71 631 605 3318 1525 Fragments arranged by size: 3318 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3495 6374 3495 Size: 2879 3500 BlpI GC'TnA_GC Cuts at: 3796 3796 Size: 6379 Bme1580I G_kGCm'C Cuts at: 1790 4440 5596 1790 Size: 2650 1156 2573 Fragments arranged by size: 2650 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2194 1318 Size: 876 5503 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2023 4110 5068 Size: 416 53 83 399 24 606 2087 958 Cuts at: 5068 442 Size: 1753 Fragments arranged by size: 2087 1753 958 606 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6379 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2083 2083 Size: 6379 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2678 2960 3335 5039 1239 Size: 1439 282 375 1704 2579 Fragments arranged by size: 2579 1704 1439 375 282 Bpu10I CC'TnA_GC Cuts at: 3585 3585 Size: 6379 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3401 3588 4504 5369 5667 3401 Size: 187 916 865 298 4113 Fragments arranged by size: 4113 916 865 298 187 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2648 5042 882 Size: 617 1149 2394 2219 Fragments arranged by size: 2394 2219 1149 617 BsaAI yAC'GTr Cuts at: 493 1204 2305 2866 4170 493 Size: 711 1101 561 1304 2702 Fragments arranged by size: 2702 1304 1101 711 561 BsaBI GATnn'nnATC Cuts at: 6373 6373 Size: 6379 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2457 2933 4186 4568 Size: 53 83 186 936 924 476 1253 382 Cuts at: 4568 5203 275 Size: 635 1451 Fragments arranged by size: 1451 1253 936 924 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2319 2412 4812 5210 5484 5631 6042 2319 Size: 93 2400 398 274 147 411 2656 Fragments arranged by size: 2656 2400 411 398 274 147 93 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2240 2270 3459 3489 1178 Size: 30 165 30 837 30 1189 30 4068 Fragments arranged by size: 4068 1189 837 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3347 1186 Size: 2161 4218 BseYI C'CCAG_C Cuts at: 2595 2971 3240 3553 5582 6078 2595 Size: 376 269 313 2029 496 2896 Fragments arranged by size: 2896 2029 496 376 313 269 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2285 2846 3023 1163 Size: 573 549 561 177 4519 Fragments arranged by size: 4519 573 561 549 177 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3829 4590 5618 1540 Size: 275 186 1828 761 1028 2301 Fragments arranged by size: 2301 1828 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 3351 3492 3604 4440 4525 5596 2064 Size: 1287 141 112 836 85 1071 2847 Fragments arranged by size: 2847 1287 1071 836 141 112 85 BsmI GAATG_Cn' Cuts at: 2671 3890 3983 2671 Size: 1219 93 5067 Fragments arranged by size: 5067 1219 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2666 2666 Size: 6379 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2905 3351 3492 3604 3639 3698 Size: 399 274 841 446 141 112 35 59 Cuts at: 3698 4440 4525 5596 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 841 742 446 399 274 141 112 85 59 35 BspEI T'CCGG_A Cuts at: 2412 6042 2412 Size: 3630 2749 BspHI T'CATG_A Cuts at: 4268 5998 4268 Size: 1730 4649 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2088 3045 3734 844 Size: 1244 957 689 3489 Fragments arranged by size: 3489 1244 957 689 BsrBI CCG'CTC Cuts at: 1394 1823 2012 2067 2251 2418 4266 1394 Size: 429 189 55 184 167 1848 3507 Fragments arranged by size: 3507 1848 429 189 184 167 55 BsrDI GCAATG_nn' Cuts at: 65 1248 2054 2577 2625 2853 4874 5048 65 Size: 1183 806 523 48 228 2021 174 1396 Fragments arranged by size: 2021 1396 1183 806 523 228 174 48 BsrFI r'CCGG_y Cuts at: 1700 1810 2319 5023 5210 1700 Size: 110 509 2704 187 2869 Fragments arranged by size: 2869 2704 509 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6379 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6379 BssSI C'ACGA_G Cuts at: 4439 5451 4439 Size: 1012 5367 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6379 BstBI TT'CG_AA Cuts at: 5183 5183 Size: 6379 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6379 BstXI CCAn_nnnn'nTGG Cuts at: 2179 2233 2590 3292 2179 Size: 54 357 702 5266 Fragments arranged by size: 5266 702 357 54 BstYI r'GATC_y Cuts at: 1972 2707 3273 3495 3709 4076 4461 4478 5930 Size: 735 566 222 214 367 385 17 1452 Cuts at: 5930 6016 6028 6374 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 735 566 385 367 346 222 214 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2146 513 Size: 552 939 142 4746 Fragments arranged by size: 4746 939 552 142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5640 Fragments arranged by size: 5640 683 56 BtsI GCAGTG_nn' Cuts at: 2724 3604 3895 4689 4709 2724 Size: 880 291 794 20 4394 Fragments arranged by size: 4394 880 794 291 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6379 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6344 DraI TTT'AAA Cuts at: 3683 3683 Size: 6379 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5386 809 Size: 507 4070 1802 Fragments arranged by size: 4070 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2217 2700 Size: 54 1425 50 275 18 177 210 483 Cuts at: 2700 2838 3163 3282 3826 4715 6344 8 Size: 138 325 119 544 889 1629 43 Fragments arranged by size: 1629 1425 889 544 483 325 275 210 177 138 119 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3826 1537 Size: 275 2014 4090 Fragments arranged by size: 4090 2014 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 2533 4309 1353 Size: 743 437 1776 3423 Fragments arranged by size: 3423 1776 743 437 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4966 5340 5486 387 Size: 1064 138 3377 374 146 1280 Fragments arranged by size: 3377 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3349 3349 Size: 6379 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2107 2514 2529 2678 2960 3335 4442 Size: 393 475 407 15 149 282 375 1107 Cuts at: 4442 5039 5826 6094 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1107 787 597 475 407 393 375 282 268 149 15 EcoO109I rG'GnC_Cy Cuts at: 2023 2492 2537 3131 3307 3376 2023 Size: 469 45 594 176 69 5026 Fragments arranged by size: 5026 594 469 176 69 45 EcoRI G'AATT_C Cuts at: 3688 4066 3688 Size: 378 6001 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2128 2128 Size: 6379 FspI TGC'GCA Cuts at: 4885 4885 Size: 6379 HaeII r_GCGC'y Cuts at: 1678 5526 1678 Size: 3848 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3062 3094 3459 3491 5020 Size: 32 135 32 1490 32 365 32 1529 Cuts at: 5020 5052 5094 5126 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1529 1490 365 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2371 3723 3969 669 Size: 988 281 433 1352 246 3079 Fragments arranged by size: 3079 1352 988 433 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6379 HpaI GTT'AAC Cuts at: 3969 3969 Size: 6379 KpnI G_GTAC'C Cuts at: 2056 3704 2056 Size: 1648 4731 MfeI C'AATT_G Cuts at: 3978 3978 Size: 6379 MluI A'CGCG_T Cuts at: 6255 6255 Size: 6379 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2508 2583 5493 5677 1305 Size: 1203 75 2910 184 2007 Fragments arranged by size: 2910 2007 1203 184 75 MscI TGG'CCA Cuts at: 10 64 1489 1832 2219 3165 3284 10 Size: 54 1425 343 387 946 119 3105 Fragments arranged by size: 3105 1425 946 387 343 119 54 MslI CAynn'nnrTG Cuts at: 518 1166 3203 4337 4696 4855 518 Size: 648 2037 1134 359 159 2042 Fragments arranged by size: 2042 2037 1134 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2599 2890 3565 3795 4472 5620 5865 1606 Size: 993 291 675 230 677 1148 245 2120 Fragments arranged by size: 2120 1148 993 677 675 291 245 230 NcoI C'CATG_G Cuts at: 513 1065 2004 2146 513 Size: 552 939 142 4746 Fragments arranged by size: 4746 939 552 142 NdeI CA'TA_TG Cuts at: 387 387 Size: 6379 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6379 NotI GC'GGCC_GC Cuts at: 3826 3826 Size: 6379 NspI r_CATG'y Cuts at: 1132 1229 2794 3173 3737 5282 1132 Size: 97 1565 379 564 1545 2229 Fragments arranged by size: 2229 1565 1545 564 379 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6379 PciI A'CATG_T Cuts at: 2790 5278 2790 Size: 2488 3891 PflMI CCAn_nnn'nTGG Cuts at: 1108 3141 1108 Size: 2033 4346 PmeI GTTT'AAAC Cuts at: 3683 3683 Size: 6379 PmlI CAC'GTG Cuts at: 2305 2866 2305 Size: 561 5818 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2240 2272 4448 4480 5961 5993 2240 Size: 32 2176 32 1481 32 2626 Fragments arranged by size: 2626 2176 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2023 2492 2537 3376 2023 Size: 469 45 839 5026 Fragments arranged by size: 5026 839 469 45 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6379 PsiI TTA'TAA Cuts at: 3949 3949 Size: 6379 PstI C_TGCA'G Cuts at: 830 2046 2719 3731 830 Size: 1216 673 1012 3478 Fragments arranged by size: 3478 1216 1012 673 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6379 PvuII CAG'CTG Cuts at: 2599 2890 3565 2599 Size: 291 675 5413 Fragments arranged by size: 5413 675 291 SacI G_AGCT'C Cuts at: 3351 3351 Size: 6379 SalI G'TCGA_C Cuts at: 1936 3721 1936 Size: 1785 4594 SanDI GG'GwC_CC Cuts at: 2023 2492 2023 Size: 469 5910 SbfI CC_TGCA'GG Cuts at: 3731 3731 Size: 6379 ScaI AGT'ACT Cuts at: 4627 6339 4627 Size: 1712 4667 SexAI A'CCwGG_T Cuts at: 2178 2178 Size: 6379 SfcI C'TryA_G Cuts at: 826 1045 2042 2715 3727 4862 5543 5734 826 Size: 219 997 673 1012 1135 681 191 1471 Fragments arranged by size: 1471 1135 1012 997 681 673 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6379 SmaI CCC'GGG Cuts at: 1872 2021 2246 3706 1872 Size: 149 225 1460 4545 Fragments arranged by size: 4545 1460 225 149 SmlI C'TyrA_G Cuts at: 820 1017 1943 2516 3380 3603 4483 5384 5646 Size: 197 926 573 864 223 880 901 262 Cuts at: 5646 820 Size: 1553 Fragments arranged by size: 1553 926 901 880 864 573 262 223 197 SnaBI TAC'GTA Cuts at: 493 4170 493 Size: 3677 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6379 SphI G_CATG'C Cuts at: 1132 3173 3737 1132 Size: 2041 564 3774 Fragments arranged by size: 3774 2041 564 SspI AAT'ATT Cuts at: 5 52 4303 5 Size: 47 4251 2081 Fragments arranged by size: 4251 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6379 StyI C'CwwG_G Cuts at: 513 1065 2004 2146 2703 3193 3471 3818 513 Size: 552 939 142 557 490 278 347 3074 Fragments arranged by size: 3074 939 557 552 490 347 278 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2347 2800 2988 4406 4423 4576 4761 Size: 1255 767 453 188 1418 17 153 185 Cuts at: 4761 5206 325 Size: 445 1498 Fragments arranged by size: 1498 1418 1255 767 453 445 188 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4625 6337 96 Size: 274 80 33 51 1443 2648 1712 138 Fragments arranged by size: 2648 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3099 3131 6339 6371 3099 Size: 32 3208 32 3107 Fragments arranged by size: 3208 3107 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6379 XbaI T'CTAG_A Cuts at: 3715 3715 Size: 6379 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6379 XmaI C'CCGG_G Cuts at: 1870 2019 2244 3704 1870 Size: 149 225 1460 4545 Fragments arranged by size: 4545 1460 225 149 XmnI GAAnn'nnTTC Cuts at: 2396 4508 2396 Size: 2112 4267 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2458 4187 276 Size: 53 83 186 936 924 1729 2468 Fragments arranged by size: 2468 1729 936 924 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI