(Linear) (Six-base) MAPSORT of: KIEE3582.seq Check: 3159 from: 1 to: 1674 KIEE3582.orf With 182 enzymes: SgfI * December 27, 2011 16:35 .. AatII G_ACGT'C Cuts at: 0 455 1674 Size: 455 1219 AccI GT'mk_AC Cuts at: 0 338 739 1674 Size: 338 401 935 Fragments arranged by size: 935 401 338 Acc65I G'GTAC_C Cuts at: 0 47 1674 Size: 47 1627 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 102 509 524 1674 Size: 102 407 15 1150 Fragments arranged by size: 1150 407 102 15 AflII C'TTAA_G Cuts at: 0 511 1674 Size: 511 1163 AflIII A'CryG_T Cuts at: 0 785 1674 Size: 785 889 AgeI A'CCGG_T Cuts at: 0 314 1674 Size: 314 1360 AhdI GACnn_n'nnGTC Cuts at: 0 1071 1674 Size: 1071 603 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 746 1058 1674 Size: 746 312 616 Fragments arranged by size: 746 616 312 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 10 42 235 267 589 621 1674 Size: 10 32 193 32 322 32 1053 Fragments arranged by size: 1053 322 193 32 32 32 10 AlwNI CAG_nnn'CTG Cuts at: 0 36 1032 1674 Size: 36 996 642 Fragments arranged by size: 996 642 36 AvaI C'yCGr_G Cuts at: 0 14 239 601 891 1674 Size: 14 225 362 290 783 Fragments arranged by size: 783 362 290 225 14 BamHI G'GATC_C Cuts at: 0 702 1674 Size: 702 972 BanI G'GyrC_C Cuts at: 0 47 1192 1674 Size: 47 1145 482 Fragments arranged by size: 1145 482 47 BanII G_rGCy'C Cuts at: 0 900 1346 1634 1674 Size: 900 446 288 40 Fragments arranged by size: 900 446 288 40 BglII A'GATC_T Cuts at: 0 1490 1674 Size: 1490 184 BmgBI CAC'GTC Cuts at: 0 189 1674 Size: 189 1485 BmrI ACTGGGnnnn_n' Cuts at: 0 18 1674 Size: 18 1656 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 78 1674 Size: 78 1596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 673 955 1330 1674 Size: 673 282 375 344 Fragments arranged by size: 673 375 344 282 Bpu10I CC'TnA_GC Cuts at: 0 1580 1674 Size: 1580 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1396 1583 1674 Size: 1396 187 91 Fragments arranged by size: 1396 187 91 BsaI GGTCTCn'nnnn_ Cuts at: 0 643 1674 Size: 643 1031 BsaAI yAC'GTr Cuts at: 0 300 861 1674 Size: 300 561 813 Fragments arranged by size: 813 561 300 BsaHI Gr'CG_yC Cuts at: 0 452 928 1674 Size: 452 476 746 Fragments arranged by size: 746 476 452 BsaWI w'CCGG_w Cuts at: 0 314 407 1674 Size: 314 93 1267 Fragments arranged by size: 1267 314 93 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 235 265 1454 1484 1674 Size: 235 30 1189 30 190 Fragments arranged by size: 1189 235 190 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1342 1674 Size: 1342 332 BseYI C'CCAG_C Cuts at: 0 590 966 1235 1548 1674 Size: 590 376 269 313 126 Fragments arranged by size: 590 376 313 269 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 841 1018 1674 Size: 280 561 177 656 Fragments arranged by size: 656 561 280 177 BsiHKAI G_wGCw'C Cuts at: 0 59 1346 1487 1599 1674 Size: 59 1287 141 112 75 Fragments arranged by size: 1287 141 112 75 59 BsmI GAATG_Cn' Cuts at: 0 666 1674 Size: 666 1008 BsmBI CGTCTCn'nnnn_ Cuts at: 0 661 1674 Size: 661 1013 Bsp1286I G_dGCh'C Cuts at: 0 59 900 1346 1487 1599 1634 1674 Size: 59 841 446 141 112 35 40 Fragments arranged by size: 841 446 141 112 59 40 35 BspEI T'CCGG_A Cuts at: 0 407 1674 Size: 407 1267 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 83 1040 1674 Size: 83 957 634 Fragments arranged by size: 957 634 83 BsrBI CCG'CTC Cuts at: 0 7 62 246 413 1674 Size: 7 55 184 167 1261 Fragments arranged by size: 1261 184 167 55 7 BsrDI GCAATG_nn' Cuts at: 0 49 572 620 848 1674 Size: 49 523 48 228 826 Fragments arranged by size: 826 523 228 49 48 BsrFI r'CCGG_y Cuts at: 0 314 1674 Size: 314 1360 BstXI CCAn_nnnn'nTGG Cuts at: 0 174 228 585 1287 1674 Size: 174 54 357 702 387 Fragments arranged by size: 702 387 357 174 54 BstYI r'GATC_y Cuts at: 0 702 1268 1490 1674 Size: 702 566 222 184 Fragments arranged by size: 702 566 222 184 BtgI C'CryG_G Cuts at: 0 141 1674 Size: 141 1533 BtsI GCAGTG_nn' Cuts at: 0 719 1599 1674 Size: 719 880 75 Fragments arranged by size: 880 719 75 EaeI y'GGCC_r Cuts at: 0 2 212 695 833 1158 1277 1674 Size: 2 210 483 138 325 119 397 Fragments arranged by size: 483 397 325 210 138 119 2 EarI CTCTTCn'nnn_ Cuts at: 0 91 528 1674 Size: 91 437 1146 Fragments arranged by size: 1146 437 91 EcoICRI GAG'CTC Cuts at: 0 1344 1674 Size: 1344 330 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 102 509 524 673 955 1330 1674 Size: 102 407 15 149 282 375 344 Fragments arranged by size: 407 375 344 282 149 102 15 EcoO109I rG'GnC_Cy Cuts at: 0 18 487 532 1126 1302 1371 1674 Size: 18 469 45 594 176 69 303 Fragments arranged by size: 594 469 303 176 69 45 18 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 123 1674 Size: 123 1551 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1057 1089 1454 1486 1674 Size: 1057 32 365 32 188 Fragments arranged by size: 1057 365 188 32 32 HincII GTy'rAC Cuts at: 0 366 1674 Size: 366 1308 KpnI G_GTAC'C Cuts at: 0 51 1674 Size: 51 1623 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 503 578 1674 Size: 503 75 1096 Fragments arranged by size: 1096 503 75 MscI TGG'CCA Cuts at: 0 214 1160 1279 1674 Size: 214 946 119 395 Fragments arranged by size: 946 395 214 119 MslI CAynn'nnrTG Cuts at: 0 1198 1674 Size: 1198 476 MspA1I CmG'CkG Cuts at: 0 594 885 1560 1674 Size: 594 291 675 114 Fragments arranged by size: 675 594 291 114 NcoI C'CATG_G Cuts at: 0 141 1674 Size: 141 1533 NspI r_CATG'y Cuts at: 0 789 1168 1674 Size: 789 379 506 Fragments arranged by size: 789 506 379 PciI A'CATG_T Cuts at: 0 785 1674 Size: 785 889 PflMI CCAn_nnn'nTGG Cuts at: 0 1136 1674 Size: 1136 538 PmlI CAC'GTG Cuts at: 0 300 861 1674 Size: 300 561 813 Fragments arranged by size: 813 561 300 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 235 267 1674 Size: 235 32 1407 Fragments arranged by size: 1407 235 32 PpuMI rG'GwC_Cy Cuts at: 0 18 487 532 1371 1674 Size: 18 469 45 839 303 Fragments arranged by size: 839 469 303 45 18 PstI C_TGCA'G Cuts at: 0 41 714 1674 Size: 41 673 960 Fragments arranged by size: 960 673 41 PvuII CAG'CTG Cuts at: 0 594 885 1560 1674 Size: 594 291 675 114 Fragments arranged by size: 675 594 291 114 SacI G_AGCT'C Cuts at: 0 1346 1674 Size: 1346 328 SanDI GG'GwC_CC Cuts at: 0 18 487 1674 Size: 18 469 1187 Fragments arranged by size: 1187 469 18 SexAI A'CCwGG_T Cuts at: 0 173 1674 Size: 173 1501 SfcI C'TryA_G Cuts at: 0 37 710 1674 Size: 37 673 964 Fragments arranged by size: 964 673 37 SmaI CCC'GGG Cuts at: 0 16 241 1674 Size: 16 225 1433 Fragments arranged by size: 1433 225 16 SmlI C'TyrA_G Cuts at: 0 511 1375 1598 1674 Size: 511 864 223 76 Fragments arranged by size: 864 511 223 76 SphI G_CATG'C Cuts at: 0 1168 1674 Size: 1168 506 StyI C'CwwG_G Cuts at: 0 141 698 1188 1466 1674 Size: 141 557 490 278 208 Fragments arranged by size: 557 490 278 208 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 342 795 983 1674 Size: 342 453 188 691 Fragments arranged by size: 691 453 342 188 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1094 1126 1674 Size: 1094 32 548 Fragments arranged by size: 1094 548 32 XmaI C'CCGG_G Cuts at: 0 14 239 1674 Size: 14 225 1435 Fragments arranged by size: 1435 225 14 XmnI GAAnn'nnTTC Cuts at: 0 391 1674 Size: 391 1283 ZraI GAC'GTC Cuts at: 0 453 1674 Size: 453 1221 Enzymes that do cut: AatII AccI Acc65I AcuI AflII AflIII AgeI AhdI AlfI AloI AlwNI AvaI BamHI BanI BanII BglII BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstXI BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII KpnI MmeI MscI MslI MspA1I NcoI NspI PciI PflMI PmlI PpiI PpuMI PstI PvuII SacI SanDI SexAI SfcI SmaI SmlI SphI StyI TaqII TstI XmaI XmnI ZraI Enzymes that do not cut: AarI AclI AfeI AleI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmtI BsaBI BsiEI BsiWI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PfoI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI