################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaII AvrII BamHI BbeI BciVI BfaI BfrI BglI BlnI BlpI Bpu1102I BseAI BsgI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI KasI Ksp632I MaeI MaeIII MfeI MroI MunI NaeI NarI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 235 GGCGTC G[GA]^CG[CT]C Aor51HI 170 AGCGCT AGC^GCT AvaI 72 CCCGGG C^[CT]CG[GA]G AviII 614 TGCGCA TGC^GCA BalI 869 TGGCCA TGG^CCA BbrPI 774 CACGTG CAC^GTG BbvCI 321 CCTCAGC CCTCAGC(-5/-2) BcgI 216 GCAGACGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 662 TGATCA T^GATCA BglII 116 AGATCT A^GATCT BpmI 751 CTGGAG CTGGAG(16/14) BsaBI 117 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 235 GGCGTC G[GA]^CG[CT]C BsaI 430 GAGACC GGTCTC(1/5) BsaWI 767 ACCGGA [AT]^CCGG[AT] BseRI 716 CTCCTC GAGGAG(10/8) BsmAI 430 GAGAC GTCTC(1/5) BsmI 820 GCATTC GAATGC(1/-1) BsoBI 72 CCCGGG C^[CT]CG[GA]G BspHI 602 TCATGA T^CATGA BssHII 21 GCGCGC G^CGCGC Eco47III 170 AGCGCT AGC^GCT FbaI 662 TGATCA T^GATCA FspI 614 TGCGCA TGC^GCA Hin1I 235 GGCGTC G[GA]^CG[CT]C HinfI 488 GATTC G^A[ACGT]TC MamI 117 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 364 ACGCGT A^CGCGT MluNI 869 TGGCCA TGG^CCA MscI 869 TGGCCA TGG^CCA MseI 731 TTAA T^TAA MslI 435 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NotI 31 GCGGCCGC GC^GGCCGC NspI 332 ACATGC [GA]CATG^[CT] PmaCI 774 CACGTG CAC^GTG PmlI 774 CACGTG CAC^GTG PstI 157 CTGCAG CTGCA^G RcaI 602 TCATGA T^CATGA SfcI 157 CTGCAG C^T[GA][CT]AG SmaI 72 CCCGGG CCC^GGG SmlI 814 CTCAAG C^T[CT][GA]AG TfiI 488 GATTC G^A[AT]TC Tru9I 731 TTAA T^TAA XmaCI 72 CCCGGG C^CCGGG XmaI 72 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Acc65I 188 GGTACC G^GTACC 623 GGTACC AflIII 364 ACGCGT A^C[GA][CT]GT 773 ACACGT AlwI 84 GATCC GGATC(4/5) 795 GATCC Asp718I 188 GGTACC G^GTACC 623 GGTACC BanI 188 GGTACC G^G[CT][GA]CC 623 GGTACC BanII 573 GGGCTC G[GA]GC[CT]^C 811 GGGCTC BsaAI 142 TACGTG [CT]AC^GT[GA] 774 CACGTG BsiEI 32 CGGCCG CG[GA][CT]^CG 293 CGGCCG BstYI 116 AGATCT [GA]^GATC[CT] 794 AGATCC DdeI 259 CTTAG C^T[ACGT]AG 322 CTCAG DraII 545 AGGGCCT [GA]G^G[ACGT]CC[CT] 746 AGGGCCT EagI 32 CGGCCG C^GGCCG 293 CGGCCG EclXI 32 CGGCCG C^GGCCG 293 CGGCCG Eco52I 32 CGGCCG C^GGCCG 293 CGGCCG EcoNI 564 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 798 CCTGAAGAAGG EcoO109I 545 AGGGCCT [GA]G^G[ACGT]CC[CT] 746 AGGGCCT HaeII 170 AGCGCT [GA]GCGC^[CT] 388 GGCGCT KpnI 188 GGTACC GGTAC^C 623 GGTACC KspI 184 CCGCGG CCGC^GG 252 CCGCGG MflI 116 AGATCT [GA]^GATC[CT] 794 AGATCC NcoI 231 CCATGG C^CATGG 425 CCATGG PvuII 154 CAGCTG CAG^CTG 160 CAGCTG SacII 184 CCGCGG CCGC^GG 252 CCGCGG XhoII 116 AGATCT [GA]^GATC[CT] 794 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI BbsI BbvI BcnI BmyI BpuAI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI TspRI TthHB8I