(Circular) (Six-base) MAPSORT of: pFN21AE6302.seq Check: 4664 from: 1 to: 5638 pFN21AE6302 5638 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3448 278 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2981 1937 Size: 1044 4594 Acc65I G'GTAC_C Cuts at: 2193 2628 2959 2193 Size: 435 331 4872 Fragments arranged by size: 4872 435 331 AclI AA'CG_TT Cuts at: 1563 3765 4138 1563 Size: 2202 373 3063 Fragments arranged by size: 3063 2202 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2555 2591 2825 3701 5085 5353 1632 Size: 923 36 234 876 1384 268 1917 Fragments arranged by size: 1917 1384 923 876 268 234 36 AfeI AGC'GCT Cuts at: 2177 2177 Size: 5638 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5441 AflIII A'CryG_T Cuts at: 1203 2369 2778 4537 5514 1203 Size: 1166 409 1759 977 1327 Fragments arranged by size: 1759 1327 1166 977 409 AgeI A'CCGG_T Cuts at: 4469 4469 Size: 5638 AhdI GACnn_n'nnGTC Cuts at: 4367 4367 Size: 5638 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5638 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2354 1184 Size: 1170 4468 AlwNI CAG_nnn'CTG Cuts at: 2564 2755 2803 3381 4953 2564 Size: 191 48 578 1572 3249 Fragments arranged by size: 3249 1572 578 191 48 ApaLI G'TGCA_C Cuts at: 3695 4851 3695 Size: 1156 4482 ApoI r'AATT_y Cuts at: 1493 2947 3173 3325 5434 1493 Size: 1454 226 152 2109 1697 Fragments arranged by size: 2109 1697 1454 226 152 AseI AT'TA_AT Cuts at: 160 1032 4192 160 Size: 872 3160 1606 Fragments arranged by size: 3160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 AvaI C'yCGr_G Cuts at: 1870 1943 2077 2963 1870 Size: 73 134 886 4545 Fragments arranged by size: 4545 886 134 73 BamHI G'GATC_C Cuts at: 2968 3335 2968 Size: 367 5271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2193 2628 2959 4414 618 Size: 325 217 627 406 435 331 1455 1842 Fragments arranged by size: 1842 1455 627 435 406 331 325 217 BanII G_rGCy'C Cuts at: 1391 2582 2820 2957 1391 Size: 1191 238 137 4072 Fragments arranged by size: 4072 1191 238 137 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2159 2671 2936 928 Size: 971 260 512 265 3630 Fragments arranged by size: 3630 971 512 265 260 BbvCI CC'TCA_GC Cuts at: 2327 2327 Size: 5638 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2210 2244 3829 3863 1903 Size: 34 50 34 189 34 1585 34 3678 Fragments arranged by size: 3678 1585 189 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3530 4740 3530 Size: 1210 4428 BclI T'GATC_A Cuts at: 1548 2667 1548 Size: 1119 4519 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4249 136 Size: 107 122 71 631 605 2577 1525 Fragments arranged by size: 2577 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2121 5633 2121 Size: 3512 2126 BlpI GC'TnA_GC Cuts at: 3055 3055 Size: 5638 Bme1580I G_kGCm'C Cuts at: 1790 2429 2840 3699 4855 1790 Size: 639 411 859 1156 2573 Fragments arranged by size: 2573 1156 859 639 411 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5638 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2682 3369 4327 Size: 416 53 83 399 24 1265 687 958 Cuts at: 4327 442 Size: 1753 Fragments arranged by size: 1753 1265 958 687 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2777 4298 1239 Size: 1538 1521 2579 Fragments arranged by size: 2579 1538 1521 Bpu10I CC'TnA_GC Cuts at: 2264 2327 2264 Size: 63 5575 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2804 3763 4628 4926 2804 Size: 959 865 298 3516 Fragments arranged by size: 3516 959 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2429 4301 882 Size: 617 930 1872 2219 Fragments arranged by size: 2219 1872 930 617 BsaAI yAC'GTr Cuts at: 493 1204 2149 2781 3429 493 Size: 711 945 632 648 2702 Fragments arranged by size: 2702 945 711 648 632 BsaBI GATnn'nnATC Cuts at: 2126 5632 2126 Size: 3506 2132 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2241 3445 3827 4462 Size: 53 83 186 936 708 1204 382 635 Cuts at: 4462 275 Size: 1451 Fragments arranged by size: 1451 1204 936 708 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2772 4071 4469 4743 4890 5301 2772 Size: 1299 398 274 147 411 3109 Fragments arranged by size: 3109 1299 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2453 2483 1178 Size: 30 165 30 1050 30 4333 Fragments arranged by size: 4333 1050 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2712 1186 Size: 1526 4112 BseYI C'CCAG_C Cuts at: 4841 5337 4841 Size: 496 5142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5065 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2040 2301 3088 3849 4877 1540 Size: 275 186 39 261 787 761 1028 2301 Fragments arranged by size: 2301 1028 787 761 275 261 186 39 BsiHKAI G_wGCw'C Cuts at: 3699 3784 4855 3699 Size: 85 1071 4482 Fragments arranged by size: 4482 1071 85 BsmI GAATG_Cn' Cuts at: 2825 3149 3242 2825 Size: 324 93 5221 Fragments arranged by size: 5221 324 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2429 2582 2820 2840 2957 3699 3784 Size: 399 639 153 238 20 117 742 85 Cuts at: 3784 4855 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 639 399 238 153 117 85 20 BspEI T'CCGG_A Cuts at: 5301 5301 Size: 5638 BspHI T'CATG_A Cuts at: 2607 3527 5257 2607 Size: 920 1730 2988 Fragments arranged by size: 2988 1730 920 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2993 844 Size: 2149 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3525 1394 Size: 429 1702 3507 Fragments arranged by size: 3507 1702 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4133 4307 65 Size: 1183 2885 174 1396 Fragments arranged by size: 2885 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4282 4469 1700 Size: 110 2472 187 2869 Fragments arranged by size: 2869 2472 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5638 BssHII G'CGCG_C Cuts at: 1925 2026 1925 Size: 101 5537 BssSI C'ACGA_G Cuts at: 3698 4710 3698 Size: 1012 4626 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5638 BstBI TT'CG_AA Cuts at: 4442 4442 Size: 5638 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5638 BstYI r'GATC_y Cuts at: 1972 2121 2799 2968 3335 3720 3737 5189 5275 Size: 149 678 169 367 385 17 1452 86 Cuts at: 5275 5287 5633 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 678 385 367 346 169 149 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2189 2236 2257 2412 2430 2500 513 Size: 552 1124 47 21 155 18 70 3651 Fragments arranged by size: 3651 1124 552 155 70 47 21 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4899 Fragments arranged by size: 4899 683 56 BtsI GCAGTG_nn' Cuts at: 3154 3948 3968 3154 Size: 794 20 4824 Fragments arranged by size: 4824 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5603 DraI TTT'AAA Cuts at: 2942 2942 Size: 5638 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4645 809 Size: 507 3329 1802 Fragments arranged by size: 3329 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2298 2403 Size: 54 1425 50 275 18 207 261 105 Cuts at: 2403 2874 3085 3974 5603 8 Size: 471 211 889 1629 43 Fragments arranged by size: 1629 1425 889 471 275 261 211 207 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2037 2298 3085 1537 Size: 275 225 261 787 4090 Fragments arranged by size: 4090 787 275 261 225 EarI CTCTTCn'nnn_ Cuts at: 1353 3568 1353 Size: 2215 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4225 4599 4745 387 Size: 1064 138 2636 374 146 1280 Fragments arranged by size: 2636 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2555 2591 2777 2825 3701 4298 5085 Size: 393 923 36 186 48 876 597 787 Cuts at: 5085 5353 1239 Size: 268 1524 Fragments arranged by size: 1524 923 876 787 597 393 268 186 48 36 EcoNI CCTnn'n_nnAGG Cuts at: 2573 2807 2573 Size: 234 5404 EcoO109I rG'GnC_Cy Cuts at: 2551 2752 2551 Size: 201 5437 EcoRI G'AATT_C Cuts at: 2947 3325 2947 Size: 378 5260 FspI TGC'GCA Cuts at: 2621 4144 2621 Size: 1523 4115 HaeII r_GCGC'y Cuts at: 1678 2179 2397 4785 1678 Size: 501 218 2388 2531 Fragments arranged by size: 2531 2388 501 218 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2162 2194 2453 2485 4279 Size: 32 135 32 590 32 259 32 1794 Cuts at: 4279 4311 4353 4385 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1794 590 259 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2982 3228 669 Size: 988 281 1044 246 3079 Fragments arranged by size: 3079 1044 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5638 HpaI GTT'AAC Cuts at: 3228 3228 Size: 5638 KpnI G_GTAC'C Cuts at: 2197 2632 2963 2197 Size: 435 331 4872 Fragments arranged by size: 4872 435 331 MfeI C'AATT_G Cuts at: 3237 3237 Size: 5638 MluI A'CGCG_T Cuts at: 2369 5514 2369 Size: 3145 2493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4752 4936 1305 Size: 3447 184 2007 Fragments arranged by size: 3447 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2876 10 Size: 54 1425 343 1044 2772 Fragments arranged by size: 2772 1425 1044 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2444 3596 3955 4114 518 Size: 648 1278 1152 359 159 2042 Fragments arranged by size: 2042 1278 1152 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2161 2167 2191 2259 3054 3731 4879 5124 Size: 555 6 24 68 795 677 1148 245 Cuts at: 5124 1606 Size: 2120 Fragments arranged by size: 2120 1148 795 677 555 245 68 24 6 NcoI C'CATG_G Cuts at: 513 1065 2236 2430 513 Size: 552 1171 194 3721 Fragments arranged by size: 3721 1171 552 194 NdeI CA'TA_TG Cuts at: 387 387 Size: 5638 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5638 NotI GC'GGCC_GC Cuts at: 2037 3085 2037 Size: 1048 4590 NspI r_CATG'y Cuts at: 1132 1229 2341 2996 4541 1132 Size: 97 1112 655 1545 2229 Fragments arranged by size: 2229 1545 1112 655 97 PasI CC'CwG_GG Cuts at: 1348 2350 1348 Size: 1002 4636 PciI A'CATG_T Cuts at: 4537 4537 Size: 5638 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5638 PfoI T'CCnGG_A Cuts at: 2054 2054 Size: 5638 PmeI GTTT'AAAC Cuts at: 2942 2942 Size: 5638 PmlI CAC'GTG Cuts at: 2781 2781 Size: 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3707 3739 5220 5252 3707 Size: 32 1481 32 4093 Fragments arranged by size: 4093 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5638 PsiI TTA'TAA Cuts at: 3208 3208 Size: 5638 PstI C_TGCA'G Cuts at: 830 2166 2990 830 Size: 1336 824 3478 Fragments arranged by size: 3478 1336 824 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5638 PvuII CAG'CTG Cuts at: 2161 2167 2161 Size: 6 5632 SacII CC_GC'GG Cuts at: 2192 2260 2192 Size: 68 5570 SalI G'TCGA_C Cuts at: 1936 2980 1936 Size: 1044 4594 SbfI CC_TGCA'GG Cuts at: 2990 2990 Size: 5638 ScaI AGT'ACT Cuts at: 3886 5598 3886 Size: 1712 3926 SfcI C'TryA_G Cuts at: 826 1045 2162 2986 4121 4802 4993 826 Size: 219 1117 824 1135 681 191 1471 Fragments arranged by size: 1471 1135 1117 824 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5638 SmaI CCC'GGG Cuts at: 1872 2079 2965 1872 Size: 207 886 4545 Fragments arranged by size: 4545 886 207 SmlI C'TyrA_G Cuts at: 820 1017 1943 2819 3742 4643 4905 820 Size: 197 926 876 923 901 262 1553 Fragments arranged by size: 1553 926 923 901 876 262 197 SnaBI TAC'GTA Cuts at: 493 3429 493 Size: 2936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5638 SphI G_CATG'C Cuts at: 1132 2996 1132 Size: 1864 3774 SspI AAT'ATT Cuts at: 5 52 3562 5 Size: 47 3510 2081 Fragments arranged by size: 3510 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5638 StyI C'CwwG_G Cuts at: 513 1065 2236 2292 2430 3077 513 Size: 552 1171 56 138 647 3074 Fragments arranged by size: 3074 1171 647 552 138 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3665 3682 3835 4020 4465 325 Size: 1255 2085 17 153 185 445 1498 Fragments arranged by size: 2085 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2484 3884 5596 Size: 274 80 33 51 1443 507 1400 1712 Cuts at: 5596 96 Size: 138 Fragments arranged by size: 1712 1443 1400 507 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5598 5630 5598 Size: 32 5606 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5638 XbaI T'CTAG_A Cuts at: 2974 2974 Size: 5638 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5638 XmaI C'CCGG_G Cuts at: 1870 2077 2963 1870 Size: 207 886 4545 Fragments arranged by size: 4545 886 207 XmnI GAAnn'nnTTC Cuts at: 3767 3767 Size: 5638 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3446 276 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI