################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AfaI AflII AgeI AhdI Alw44I ApaI ApaLI ApoI AscI Asp718I AspEI AspI AviII AvrII BamHI BanII BbrPI BbvCI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeII MaeIII MamI MfeI MluI MunI NaeI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcyI 67 GGCGCC G[GA]^CG[CT]C AflIII 653 ACATGT A^C[GA][CT]GT AocI 664 CCTCAGG CC^T[ACGT]AGG AseI 186 ATTAAT AT^TAAT AsnI 186 ATTAAT AT^TAAT Asp700I 18 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 872 CTCGGG C^[CT]CG[GA]G BalI 726 TGGCCA TGG^CCA BbeI 67 GGCGCC GGCGC^C BsaHI 67 GGCGCC G[GA]^CG[CT]C BsmFI 980 GGGAC GGGAC(10/14) BsoBI 872 CTCGGG C^[CT]CG[GA]G BspLU11I 653 ACATGT A^CATGT BspMI 389 ACCTGC ACCTGC(4/8) Bsu36I 664 CCTCAGG CC^T[ACGT]AGG DraIII 1137 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 253 GACCAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 726 TGGCCA [CT]^GGCC[GA] Eco81I 664 CCTCAGG CC^T[ACGT]AGG EcoNI 200 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 58 GCGTC GACGC(5/10) Hin1I 67 GGCGCC G[GA]^CG[CT]C HinfI 1190 GAATC G^A[ACGT]TC KasI 67 GGCGCC G^GCGCC MluNI 726 TGGCCA TGG^CCA MscI 726 TGGCCA TGG^CCA NarI 67 GGCGCC GG^CGCC NcoI 671 CCATGG C^CATGG NheI 1098 GCTAGC G^CTAGC PflMI 671 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 186 ATTAAT AT^TAAT PstI 1177 CTGCAG CTGCA^G SfcI 1177 CTGCAG C^T[GA][CT]AG SfiI 744 GGCCGAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 67 GGCGCC GGC^GCC TfiI 1190 GAATC G^A[AT]TC Van91I 671 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1186 TCTAGA T^CTAGA XcmI 224 CCAGGACCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 18 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AlwNI 490 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 758 CAGCAGCTG Aor51HI 122 AGCGCT AGC^GCT 501 AGCGCT AspHI 140 GTGCTC G[AT]GC[AT]^C 284 GTGCTC BanI 67 GGCGCC G^G[CT][GA]CC 660 GGTGCC BbsI 697 GAAGAC GAAGAC(2/6) 1108 GAAGAC BcgI 539 CGAATGCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1088 CGAAAACATTGC BcnI 411 CCGGG CC^[GC]GG 1059 CCCGG BfaI 1099 CTAG C^TAG 1187 CTAG BglII 20 AGATCT A^GATCT 1181 AGATCT BmyI 140 GTGCTC G[AGT]GC[ACT]^C 284 GTGCTC BpuAI 697 GAAGAC GAAGAC(2/6) 1108 GAAGAC BsiHKAI 140 GTGCTC G[AT]GC[AT]^C 284 GTGCTC BsmI 540 GAATGC GAATGC(1/-1) 954 GAATGC Bsp1286I 140 GTGCTC G[AGT]GC[ACT]^C 284 GTGCTC BsrDI 1094 CATTGC GCAATG(2/0) 544 GCAATG BsrI 465 ACTGG ACTGG(1/-1) 1140 CCAGT DraII 635 AGGCCCT [GA]G^G[ACGT]CC[CT] 1164 GGGGCCT EarI 719 CTCTTC CTCTTC(1/4) 238 GAAGAG Eco47III 122 AGCGCT AGC^GCT 501 AGCGCT EcoO109I 635 AGGCCCT [GA]G^G[ACGT]CC[CT] 1164 GGGGCCT EcoT14I 632 CCAAGG C^C[AT][AT]GG 671 CCATGG HphI 918 GGTGA GGTGA(8/7) 292 TCACC Ksp632I 719 CTCTTC CTCTTC(1/4) 238 GAAGAG KspI 416 CCGCGG CCGC^GG 779 CCGCGG MaeI 1099 CTAG C^TAG 1187 CTAG MseI 187 TTAA T^TAA 530 TTAA NciI 411 CCGGG CC^[GC]GG 1059 CCCGG NspI 653 ACATGT [GA]CATG^[CT] 1112 ACATGC SacII 416 CCGCGG CCGC^GG 779 CCGCGG SmlI 115 CTCAAG C^T[CT][GA]AG 130 CTCAAG StyI 632 CCAAGG C^C[AT][AT]GG 671 CCATGG Tru9I 187 TTAA T^TAA 530 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AccIII AciI AluI AlwI AvaII BbvI BglI BpmI BsaJI BsaWI BseAI BseRI BsgI BsiYI BslI BsmAI BspEI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboI MboII MflI MnlI MroI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp509I TspRI TthHB8I XhoII