(Circular) (Six-base) MAPSORT of: pFN21AE6335.seq Check: 2400 from: 1 to: 5920 pFN21AE6335 5920 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3730 278 Size: 53 83 186 936 2194 2468 Fragments arranged by size: 2468 2194 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3263 1937 Size: 1326 4594 Acc65I G'GTAC_C Cuts at: 3241 3241 Size: 5920 AclI AA'CG_TT Cuts at: 1563 4047 4420 1563 Size: 2484 373 3063 Fragments arranged by size: 3063 2484 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2630 3983 5367 5635 1632 Size: 998 1353 1384 268 1917 Fragments arranged by size: 1917 1384 1353 998 268 AfeI AGC'GCT Cuts at: 2129 2508 2129 Size: 379 5541 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5723 AflIII A'CryG_T Cuts at: 1203 2658 4819 5796 1203 Size: 1455 2161 977 1327 Fragments arranged by size: 2161 1455 1327 977 AgeI A'CCGG_T Cuts at: 4751 4751 Size: 5920 AhdI GACnn_n'nnGTC Cuts at: 4649 4649 Size: 5920 AleI CACnn'nnGTG Cuts at: 1166 2108 1166 Size: 942 4978 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2060 1184 Size: 876 5044 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2891 2923 2891 Size: 32 5888 AlwNI CAG_nnn'CTG Cuts at: 2500 2768 3663 5235 2500 Size: 268 895 1572 3185 Fragments arranged by size: 3185 1572 895 268 ApaLI G'TGCA_C Cuts at: 3977 5133 3977 Size: 1156 4764 ApoI r'AATT_y Cuts at: 1493 3229 3455 3607 5716 1493 Size: 1736 226 152 2109 1697 Fragments arranged by size: 2109 1736 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2192 4474 160 Size: 872 1160 2282 1606 Fragments arranged by size: 2282 1606 1160 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5920 AvaI C'yCGr_G Cuts at: 1870 1943 2877 3245 1870 Size: 73 934 368 4545 Fragments arranged by size: 4545 934 368 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2459 2492 2459 Size: 33 5887 BamHI G'GATC_C Cuts at: 3250 3617 3250 Size: 367 5553 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2665 3241 4696 618 Size: 325 217 627 285 593 576 1455 1842 Fragments arranged by size: 1842 1455 627 593 576 325 285 217 BanII G_rGCy'C Cuts at: 1391 3239 1391 Size: 1848 4072 BbeI G_GCGC'C Cuts at: 2076 2076 Size: 5920 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2709 3120 928 Size: 971 810 411 3728 Fragments arranged by size: 3728 971 810 411 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2533 2567 3082 3116 4111 4145 1903 Size: 34 596 34 515 34 995 34 3678 Fragments arranged by size: 3678 995 596 515 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3812 5022 3812 Size: 1210 4710 BclI T'GATC_A Cuts at: 1548 1548 Size: 5920 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2639 2756 2777 Size: 107 122 71 631 605 967 117 21 Cuts at: 2777 4531 136 Size: 1754 1525 Fragments arranged by size: 1754 1525 967 631 605 122 117 107 71 21 BglII A'GATC_T Cuts at: 2025 3186 5915 2025 Size: 1161 2729 2030 Fragments arranged by size: 2729 2030 1161 BlpI GC'TnA_GC Cuts at: 3337 3337 Size: 5920 Bme1580I G_kGCm'C Cuts at: 1790 3981 5137 1790 Size: 2191 1156 2573 Fragments arranged by size: 2573 2191 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5920 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3139 3651 4609 Size: 416 53 83 399 24 1722 512 958 Cuts at: 4609 442 Size: 1753 Fragments arranged by size: 1753 1722 958 512 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3107 1056 Size: 2051 3869 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2122 2122 Size: 5920 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2343 3143 4580 1239 Size: 866 238 800 1437 2579 Fragments arranged by size: 2579 1437 866 800 238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2105 2120 4045 4910 5208 2105 Size: 15 1925 865 298 2817 Fragments arranged by size: 2817 1925 865 298 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4583 882 Size: 617 3084 2219 Fragments arranged by size: 3084 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3711 493 Size: 711 2507 2702 Fragments arranged by size: 2702 2507 711 BsaBI GATnn'nnATC Cuts at: 5914 5914 Size: 5920 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 3727 4109 4744 Size: 53 83 186 936 540 1654 382 635 Cuts at: 4744 275 Size: 1451 Fragments arranged by size: 1654 1451 936 635 540 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2460 2620 2847 2988 3001 4353 4751 5025 5172 Size: 160 227 141 13 1352 398 274 147 Cuts at: 5172 5583 2460 Size: 411 2797 Fragments arranged by size: 2797 1352 411 398 274 227 160 147 141 13 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2384 2414 2891 2921 1178 Size: 30 165 30 981 30 477 30 4177 Fragments arranged by size: 4177 981 477 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2102 2822 3020 1186 Size: 847 69 720 198 4086 Fragments arranged by size: 4086 847 720 198 69 BseYI C'CCAG_C Cuts at: 2980 3076 5123 5619 2980 Size: 96 2047 496 3281 Fragments arranged by size: 3281 2047 496 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2033 2036 3092 1163 Size: 573 297 3 1056 3991 Fragments arranged by size: 3991 1056 573 297 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3370 4131 5159 1540 Size: 275 186 1369 761 1028 2301 Fragments arranged by size: 2301 1369 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2149 2293 3981 4066 5137 2149 Size: 144 1688 85 1071 2932 Fragments arranged by size: 2932 1688 1071 144 85 BsmI GAATG_Cn' Cuts at: 2551 2965 3431 3524 2551 Size: 414 466 93 4947 Fragments arranged by size: 4947 466 414 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2149 2293 3239 3981 4066 5137 1391 Size: 399 359 144 946 742 85 1071 2174 Fragments arranged by size: 2174 1071 946 742 399 359 144 85 BspEI T'CCGG_A Cuts at: 2460 2620 2847 3001 5583 2460 Size: 160 227 154 2582 2797 Fragments arranged by size: 2797 2582 227 160 154 BspHI T'CATG_A Cuts at: 3809 5539 3809 Size: 1730 4190 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2403 3275 844 Size: 1559 872 3489 Fragments arranged by size: 3489 1559 872 BsrBI CCG'CTC Cuts at: 1394 1823 3807 1394 Size: 429 1984 3507 Fragments arranged by size: 3507 1984 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2556 3098 4415 4589 65 Size: 1183 1308 542 1317 174 1396 Fragments arranged by size: 1396 1317 1308 1183 542 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4564 4751 1700 Size: 110 2754 187 2869 Fragments arranged by size: 2869 2754 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5920 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5920 BssSI C'ACGA_G Cuts at: 3980 4992 3980 Size: 1012 4908 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5920 BstBI TT'CG_AA Cuts at: 4724 4724 Size: 5920 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5920 BstYI r'GATC_y Cuts at: 1972 2025 2164 2844 3186 3250 3617 4002 4019 Size: 53 139 680 342 64 367 385 17 Cuts at: 4019 5471 5557 5569 5915 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 680 385 367 346 342 139 86 64 53 17 12 Bsu36I CC'TnA_GG Cuts at: 2670 2670 Size: 5920 BtgI C'CryG_G Cuts at: 513 1065 2421 2676 2784 513 Size: 552 1356 255 108 3649 Fragments arranged by size: 3649 1356 552 255 108 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5181 Fragments arranged by size: 5181 683 56 BtsI GCAGTG_nn' Cuts at: 3436 4230 4250 3436 Size: 794 20 5106 Fragments arranged by size: 5106 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5920 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5885 DraI TTT'AAA Cuts at: 3224 3224 Size: 5920 DraIII CAC_nnn'GTG Cuts at: 3147 3147 Size: 5920 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2264 4927 809 Size: 507 948 2663 1802 Fragments arranged by size: 2663 1802 948 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2731 3367 4256 Size: 54 1425 50 275 18 901 636 889 Cuts at: 4256 5885 8 Size: 1629 43 Fragments arranged by size: 1629 1425 901 889 636 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3367 1537 Size: 275 1555 4090 Fragments arranged by size: 4090 1555 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2238 2730 3850 1353 Size: 885 492 1120 3423 Fragments arranged by size: 3423 1120 885 492 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4507 4881 5027 387 Size: 1064 138 2918 374 146 1280 Fragments arranged by size: 2918 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2343 2630 3143 3983 4580 5367 Size: 393 473 238 287 513 840 597 787 Cuts at: 5367 5635 1239 Size: 268 1524 Fragments arranged by size: 1524 840 787 597 513 473 393 287 268 238 EcoNI CCTnn'n_nnAGG Cuts at: 2209 2209 Size: 5920 EcoO109I rG'GnC_Cy Cuts at: 2641 3170 2641 Size: 529 5391 EcoRI G'AATT_C Cuts at: 3229 3607 3229 Size: 378 5542 FspI TGC'GCA Cuts at: 4426 4426 Size: 5920 HaeII r_GCGC'y Cuts at: 1678 2076 2131 2510 2799 5067 1678 Size: 398 55 379 289 2268 2531 Fragments arranged by size: 2531 2268 398 379 289 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3142 3174 3196 3228 4561 Size: 32 135 32 1570 32 22 32 1333 Cuts at: 4561 4593 4635 4667 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1570 1333 135 42 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3264 3510 669 Size: 988 281 1326 246 3079 Fragments arranged by size: 3079 1326 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5920 HpaI GTT'AAC Cuts at: 3510 3510 Size: 5920 KasI G'GCGC_C Cuts at: 2072 2072 Size: 5920 KpnI G_GTAC'C Cuts at: 3245 3245 Size: 5920 MfeI C'AATT_G Cuts at: 3519 3519 Size: 5920 MluI A'CGCG_T Cuts at: 5796 5796 Size: 5920 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 5034 5218 1305 Size: 786 2943 184 2007 Fragments arranged by size: 2943 2007 786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2733 10 Size: 54 1425 343 901 3197 Fragments arranged by size: 3197 1425 901 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2108 2486 2609 2663 3878 4237 Size: 648 843 99 378 123 54 1215 359 Cuts at: 4237 4396 518 Size: 159 2042 Fragments arranged by size: 2042 1215 843 648 378 359 159 123 99 54 MspA1I CmG'CkG Cuts at: 1606 2423 2650 2768 2786 3076 3336 4013 5161 Size: 817 227 118 18 290 260 677 1148 Cuts at: 5161 5406 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 817 677 290 260 245 227 118 18 NarI GG'CG_CC Cuts at: 2073 2073 Size: 5920 NcoI C'CATG_G Cuts at: 513 1065 2676 513 Size: 552 1611 3757 Fragments arranged by size: 3757 1611 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5920 NheI G'CTAG_C Cuts at: 1052 3103 1052 Size: 2051 3869 NotI GC'GGCC_GC Cuts at: 3367 3367 Size: 5920 NspI r_CATG'y Cuts at: 1132 1229 2662 3121 3278 4823 1132 Size: 97 1433 459 157 1545 2229 Fragments arranged by size: 2229 1545 1433 459 157 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5920 PciI A'CATG_T Cuts at: 2658 4819 2658 Size: 2161 3759 PflMI CCAn_nnn'nTGG Cuts at: 1108 2682 1108 Size: 1574 4346 PfoI T'CCnGG_A Cuts at: 2238 2253 3054 2238 Size: 15 801 5104 Fragments arranged by size: 5104 801 15 PmeI GTTT'AAAC Cuts at: 3224 3224 Size: 5920 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2891 2923 3989 4021 5502 5534 2891 Size: 32 1066 32 1481 32 3277 Fragments arranged by size: 3277 1481 1066 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5920 PsiI TTA'TAA Cuts at: 3490 3490 Size: 5920 PstI C_TGCA'G Cuts at: 830 3186 3272 830 Size: 2356 86 3478 Fragments arranged by size: 3478 2356 86 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5920 PvuII CAG'CTG Cuts at: 2650 2768 3076 2650 Size: 118 308 5494 Fragments arranged by size: 5494 308 118 SacII CC_GC'GG Cuts at: 2424 2787 2424 Size: 363 5557 SalI G'TCGA_C Cuts at: 1936 3262 1936 Size: 1326 4594 SbfI CC_TGCA'GG Cuts at: 3272 3272 Size: 5920 ScaI AGT'ACT Cuts at: 4168 5880 4168 Size: 1712 4208 SfcI C'TryA_G Cuts at: 826 1045 3182 3268 4403 5084 5275 826 Size: 219 2137 86 1135 681 191 1471 Fragments arranged by size: 2137 1471 1135 681 219 191 86 SfiI GGCCn_nnn'nGGCC Cuts at: 2756 2756 Size: 5920 SfoI GGC'GCC Cuts at: 2074 2074 Size: 5920 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5920 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5920 SmaI CCC'GGG Cuts at: 1872 3247 1872 Size: 1375 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2120 2135 4024 4925 5187 820 Size: 197 926 177 15 1889 901 262 1553 Fragments arranged by size: 1889 1553 926 901 262 197 177 15 SnaBI TAC'GTA Cuts at: 493 3711 493 Size: 3218 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5920 SphI G_CATG'C Cuts at: 1132 3278 1132 Size: 2146 3774 SspI AAT'ATT Cuts at: 5 52 3844 5 Size: 47 3792 2081 Fragments arranged by size: 3792 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5920 StyI C'CwwG_G Cuts at: 513 1065 2637 2676 3359 513 Size: 552 1572 39 683 3074 Fragments arranged by size: 3074 1572 683 552 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3158 3947 3964 4117 4302 4747 325 Size: 1255 1578 789 17 153 185 445 1498 Fragments arranged by size: 1578 1498 1255 789 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4166 5878 96 Size: 274 80 33 51 1443 2189 1712 138 Fragments arranged by size: 2189 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2710 2742 5880 5912 2710 Size: 32 3138 32 2718 Fragments arranged by size: 3138 2718 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5920 XbaI T'CTAG_A Cuts at: 3191 3256 3191 Size: 65 5855 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2236 2236 Size: 5920 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5920 XmaI C'CCGG_G Cuts at: 1870 3245 1870 Size: 1375 4545 XmnI GAAnn'nnTTC Cuts at: 2027 4049 2027 Size: 2022 3898 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3728 276 Size: 53 83 186 936 2194 2468 Fragments arranged by size: 2468 2194 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI