(Linear) (Six-base) MAPSORT of: KIEE6335.seq Check: 4267 from: 1 to: 1215 KIEE6335.orf With 182 enzymes: SgfI * March 16, 2015 11:30 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 625 1215 Size: 625 590 AfeI AGC'GCT Cuts at: 0 124 503 1215 Size: 124 379 712 Fragments arranged by size: 712 379 124 AflIII A'CryG_T Cuts at: 0 653 1215 Size: 653 562 AleI CACnn'nnGTG Cuts at: 0 103 1215 Size: 103 1112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 55 1215 Size: 55 1160 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 886 918 1215 Size: 886 32 297 Fragments arranged by size: 886 297 32 AlwNI CAG_nnn'CTG Cuts at: 0 495 763 1215 Size: 495 268 452 Fragments arranged by size: 495 452 268 AseI AT'TA_AT Cuts at: 0 187 1215 Size: 187 1028 AvaI C'yCGr_G Cuts at: 0 872 1215 Size: 872 343 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 454 487 1215 Size: 454 33 728 Fragments arranged by size: 728 454 33 BanI G'GyrC_C Cuts at: 0 67 660 1215 Size: 67 593 555 Fragments arranged by size: 593 555 67 BbeI G_GCGC'C Cuts at: 0 71 1215 Size: 71 1144 BbsI GAAGACnn'nnnn_ Cuts at: 0 704 1115 1215 Size: 704 411 100 Fragments arranged by size: 704 411 100 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 528 562 1077 1111 1215 Size: 528 34 515 34 104 Fragments arranged by size: 528 515 104 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 634 751 772 1215 Size: 634 117 21 443 Fragments arranged by size: 634 443 117 21 BglII A'GATC_T Cuts at: 0 20 1181 1215 Size: 20 1161 34 Fragments arranged by size: 1161 34 20 BmrI ACTGGGnnnn_n' Cuts at: 0 1134 1215 Size: 1134 81 BmtI G_CTAG'C Cuts at: 0 1102 1215 Size: 1102 113 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 117 1215 Size: 117 1098 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 338 1138 1215 Size: 100 238 800 77 Fragments arranged by size: 800 238 100 77 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 115 1215 Size: 100 15 1100 Fragments arranged by size: 1100 100 15 BsaHI Gr'CG_yC Cuts at: 0 68 1215 Size: 68 1147 BsaWI w'CCGG_w Cuts at: 0 455 615 842 983 996 1215 Size: 455 160 227 141 13 219 Fragments arranged by size: 455 227 219 160 141 13 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 379 409 886 916 1215 Size: 379 30 477 30 299 Fragments arranged by size: 477 379 299 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 28 97 817 1015 1215 Size: 28 69 720 198 200 Fragments arranged by size: 720 200 198 69 28 BseYI C'CCAG_C Cuts at: 0 975 1071 1215 Size: 975 96 144 Fragments arranged by size: 975 144 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 28 31 1087 1215 Size: 28 3 1056 128 Fragments arranged by size: 1056 128 28 3 BsiHKAI G_wGCw'C Cuts at: 0 144 288 1215 Size: 144 144 927 Fragments arranged by size: 927 144 144 BsmI GAATG_Cn' Cuts at: 0 546 960 1215 Size: 546 414 255 Fragments arranged by size: 546 414 255 Bsp1286I G_dGCh'C Cuts at: 0 144 288 1215 Size: 144 144 927 Fragments arranged by size: 927 144 144 BspEI T'CCGG_A Cuts at: 0 455 615 842 996 1215 Size: 455 160 227 154 219 Fragments arranged by size: 455 227 219 160 154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 398 1215 Size: 398 817 BsrDI GCAATG_nn' Cuts at: 0 551 1093 1215 Size: 551 542 122 Fragments arranged by size: 551 542 122 BstYI r'GATC_y Cuts at: 0 20 159 839 1181 1215 Size: 20 139 680 342 34 Fragments arranged by size: 680 342 139 34 20 Bsu36I CC'TnA_GG Cuts at: 0 665 1215 Size: 665 550 BtgI C'CryG_G Cuts at: 0 416 671 779 1215 Size: 416 255 108 436 Fragments arranged by size: 436 416 255 108 DraIII CAC_nnn'GTG Cuts at: 0 1142 1215 Size: 1142 73 DrdI GACnn_nn'nnGTC Cuts at: 0 259 1215 Size: 259 956 EaeI y'GGCC_r Cuts at: 0 726 1215 Size: 726 489 EarI CTCTTCn'nnn_ Cuts at: 0 233 725 1215 Size: 233 492 490 Fragments arranged by size: 492 490 233 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 338 625 1138 1215 Size: 100 238 287 513 77 Fragments arranged by size: 513 287 238 100 77 EcoNI CCTnn'n_nnAGG Cuts at: 0 204 1215 Size: 204 1011 EcoO109I rG'GnC_Cy Cuts at: 0 636 1165 1215 Size: 636 529 50 Fragments arranged by size: 636 529 50 HaeII r_GCGC'y Cuts at: 0 71 126 505 794 1215 Size: 71 55 379 289 421 Fragments arranged by size: 421 379 289 71 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1137 1169 1191 1215 Size: 1137 32 22 24 Fragments arranged by size: 1137 32 24 22 KasI G'GCGC_C Cuts at: 0 67 1215 Size: 67 1148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 86 1215 Size: 86 1129 MscI TGG'CCA Cuts at: 0 728 1215 Size: 728 487 MslI CAynn'nnrTG Cuts at: 0 103 481 604 658 1215 Size: 103 378 123 54 557 Fragments arranged by size: 557 378 123 103 54 MspA1I CmG'CkG Cuts at: 0 418 645 763 781 1071 1215 Size: 418 227 118 18 290 144 Fragments arranged by size: 418 290 227 144 118 18 NarI GG'CG_CC Cuts at: 0 68 1215 Size: 68 1147 NcoI C'CATG_G Cuts at: 0 671 1215 Size: 671 544 NheI G'CTAG_C Cuts at: 0 1098 1215 Size: 1098 117 NspI r_CATG'y Cuts at: 0 657 1116 1215 Size: 657 459 99 Fragments arranged by size: 657 459 99 PciI A'CATG_T Cuts at: 0 653 1215 Size: 653 562 PflMI CCAn_nnn'nTGG Cuts at: 0 677 1215 Size: 677 538 PfoI T'CCnGG_A Cuts at: 0 233 248 1049 1215 Size: 233 15 801 166 Fragments arranged by size: 801 233 166 15 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 886 918 1215 Size: 886 32 297 Fragments arranged by size: 886 297 32 PstI C_TGCA'G Cuts at: 0 1181 1215 Size: 1181 34 PvuII CAG'CTG Cuts at: 0 645 763 1071 1215 Size: 645 118 308 144 Fragments arranged by size: 645 308 144 118 SacII CC_GC'GG Cuts at: 0 419 782 1215 Size: 419 363 433 Fragments arranged by size: 433 419 363 SfcI C'TryA_G Cuts at: 0 1177 1215 Size: 1177 38 SfiI GGCCn_nnn'nGGCC Cuts at: 0 751 1215 Size: 751 464 SfoI GGC'GCC Cuts at: 0 69 1215 Size: 69 1146 SmlI C'TyrA_G Cuts at: 0 115 130 1215 Size: 115 15 1085 Fragments arranged by size: 1085 115 15 StyI C'CwwG_G Cuts at: 0 632 671 1215 Size: 632 39 544 Fragments arranged by size: 632 544 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1153 1215 Size: 1153 62 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 705 737 1215 Size: 705 32 478 Fragments arranged by size: 705 478 32 XbaI T'CTAG_A Cuts at: 0 1186 1215 Size: 1186 29 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 231 1215 Size: 231 984 XmnI GAAnn'nnTTC Cuts at: 0 22 1215 Size: 22 1193 Enzymes that do cut: AcuI AfeI AflIII AleI AlfI AloI AlwNI AseI AvaI BaeI BanI BbeI BbsI BcgI BglI BglII BmrI BmtI BplI BpmI BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BstYI Bsu36I BtgI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI NheI NspI PciI PflMI PfoI PpiI PstI PvuII SacII SfcI SfiI SfoI SmlI StyI TaqII TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AhdI ApaI ApaLI ApoI AscI AsiSI AvrII BamHI BanII BbvCI BciVI BclI BfrBI BlpI Bme1580I BmgBI Bpu10I BsaI BsaAI BsaBI BsiEI BsiWI BsmBI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NotI NruI NsiI PacI PasI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XhoI XmaI ZraI