################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoT22I FauI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsaI RsrII SacI SacII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 753 TCCGGA T^CCGGA AocI 214 CCTCAGG CC^T[ACGT]AGG Aor51HI 944 AGCGCT AGC^GCT BalI 593 TGGCCA TGG^CCA BanI 685 GGTGCC G^G[CT][GA]CC BanII 32 GAGCCC G[GA]GC[CT]^C BbsI 384 GAAGAC GAAGAC(2/6) BclI 1073 TGATCA T^GATCA BcnI 35 CCCGG CC^[GC]GG BfaI 391 CTAG C^TAG BglI 220 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 384 GAAGAC GAAGAC(2/6) BsaBI 168 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 472 GAGACC GGTCTC(1/5) BsaWI 753 TCCGGA [AT]^CCGG[AT] BseAI 753 TCCGGA T^CCGGA BseRI 1246 GAGGAG GAGGAG(10/8) BsgI 536 GTGCAG GTGCAG(16/14) BspDI 169 ATCGAT AT^CGAT BspEI 753 TCCGGA T^CCGGA BspHI 461 TCATGA T^CATGA BsrFI 1453 ACCGGC [GA]^CCGG[CT] Bsu36I 214 CCTCAGG CC^T[ACGT]AGG Cfr10I 1453 ACCGGC [GA]^CCGG[CT] ClaI 169 ATCGAT AT^CGAT DraII 209 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 1384 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 593 TGGCCA [CT]^GGCC[GA] Eco47III 944 AGCGCT AGC^GCT Eco81I 214 CCTCAGG CC^T[ACGT]AGG EcoNI 1466 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 209 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRV 172 GATATC GAT^ATC FbaI 1073 TGATCA T^GATCA MaeI 391 CTAG C^TAG MaeII 261 ACGT A^CGT MamI 168 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 593 TGGCCA TGG^CCA MroI 753 TCCGGA T^CCGGA MscI 593 TGGCCA TGG^CCA MspA1I 910 CAGCTG C[AC]G^C[GT]G NciI 35 CCCGG CC^[GC]GG NdeI 1325 CATATG CA^TATG PpuMI 209 GGGTCCC [GA]G^G[AT]CC[CT] PstI 43 CTGCAG CTGCA^G PvuII 910 CAGCTG CAG^CTG RcaI 461 TCATGA T^CATGA SapI 123 GCTCTTC GCTCTTC(1/4) SbfI 42 CCTGCAGG CCTGCA^GG SexAI 76 ACCTGGT A^CC[AT]GGT Sse8387I 42 CCTGCAGG CCTGCA^GG StuI 420 AGGCCT AGG^CCT XcmI 224 CCAAGGCAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 139 AAATTC [GA]^AATT[CT] 735 AAATTT ApoI 139 AAATTC [GA]^AATT[CT] 735 AAATTT BamHI 38 GGATCC G^GATCC 781 GGATCC BmyI 32 GAGCCC G[AGT]GC[ACT]^C 686 GTGCCC Bsp1286I 32 GAGCCC G[AGT]GC[ACT]^C 686 GTGCCC BsrDI 246 CATTGC GCAATG(2/0) 543 CATTGC BstYI 38 GGATCC [GA]^GATC[CT] 781 GGATCC CfoI 7 GCGC GCG^C 945 GCGC EarI 99 CTCTTC CTCTTC(1/4) 124 CTCTTC HaeII 6 GGCGCT [GA]GCGC^[CT] 944 AGCGCT HhaI 7 GCGC GCG^C 945 GCGC HinP1I 7 GCGC G^CGC 945 GCGC Ksp632I 99 CTCTTC CTCTTC(1/4) 124 CTCTTC MflI 38 GGATCC [GA]^GATC[CT] 781 GGATCC MslI 591 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1422 CATTCCAGTG NspI 438 GCATGC [GA]CATG^[CT] 1014 GCATGC PflMI 986 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1383 CCACTTGGTGG SfcI 43 CTGCAG C^T[GA][CT]AG 444 CTGTAG SphI 438 GCATGC GCATG^C 1014 GCATGC TfiI 148 GAATC G^A[AT]TC 679 GATTC Tsp45I 158 GTGAC ^GT[GC]AC 451 GTGAC Van91I 986 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1383 CCACTTGGTGG XhoII 38 GGATCC [GA]^GATC[CT] 781 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BpmI BsaJI BsiYI BslI BsmAI BsmFI BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I