(Circular) (Six-base) MAPSORT of: pFN21AE6402.seq Check: 3916 from: 1 to: 6310 pFN21AE6402 6310 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3589 3589 Size: 6310 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4120 278 Size: 53 83 186 936 2584 2468 Fragments arranged by size: 2584 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3653 1937 Size: 1716 4594 Acc65I G'GTAC_C Cuts at: 3631 3631 Size: 6310 AclI AA'CG_TT Cuts at: 1563 4437 4810 1563 Size: 2874 373 3063 Fragments arranged by size: 3063 2874 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2094 2277 2414 2427 3460 4373 5757 6025 Size: 462 183 137 13 1033 913 1384 268 Cuts at: 6025 1632 Size: 1917 Fragments arranged by size: 1917 1384 1033 913 462 268 183 137 13 AfeI AGC'GCT Cuts at: 2951 2951 Size: 6310 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6113 AflIII A'CryG_T Cuts at: 1203 5209 6186 1203 Size: 4006 977 1327 Fragments arranged by size: 4006 1327 977 AgeI A'CCGG_T Cuts at: 5141 5141 Size: 6310 AhdI GACnn_n'nnGTC Cuts at: 5039 5039 Size: 6310 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6310 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6310 AlwNI CAG_nnn'CTG Cuts at: 4053 5625 4053 Size: 1572 4738 ApaLI G'TGCA_C Cuts at: 4367 5523 4367 Size: 1156 5154 ApoI r'AATT_y Cuts at: 1493 2144 2740 3619 3845 3997 6106 1493 Size: 651 596 879 226 152 2109 1697 Fragments arranged by size: 2109 1697 879 651 596 226 152 AseI AT'TA_AT Cuts at: 160 1032 4864 160 Size: 872 3832 1606 Fragments arranged by size: 3832 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6310 AvaI C'yCGr_G Cuts at: 1870 1943 3635 1870 Size: 73 1692 4545 Fragments arranged by size: 4545 1692 73 BamHI G'GATC_C Cuts at: 2043 2786 3640 4007 2043 Size: 743 854 367 4346 Fragments arranged by size: 4346 854 743 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2690 3631 5086 618 Size: 325 217 627 903 941 1455 1842 Fragments arranged by size: 1842 1455 941 903 627 325 217 BanII G_rGCy'C Cuts at: 1391 2041 3629 1391 Size: 650 1588 4072 Fragments arranged by size: 4072 1588 650 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2396 928 Size: 971 497 4842 Fragments arranged by size: 4842 971 497 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4501 4535 1903 Size: 34 2564 34 3678 Fragments arranged by size: 3678 2564 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4202 5412 4202 Size: 1210 5100 BclI T'GATC_A Cuts at: 1548 3078 1548 Size: 1530 4780 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2231 4921 Size: 107 122 71 631 605 337 222 2690 Cuts at: 4921 136 Size: 1525 Fragments arranged by size: 2690 1525 631 605 337 222 122 107 71 BglII A'GATC_T Cuts at: 6305 6305 Size: 6310 BlpI GC'TnA_GC Cuts at: 3727 3727 Size: 6310 Bme1580I G_kGCm'C Cuts at: 1790 2695 4371 5527 1790 Size: 905 1676 1156 2573 Fragments arranged by size: 2573 1676 1156 905 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6310 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4041 4999 442 Size: 416 53 83 399 24 2624 958 1753 Fragments arranged by size: 2624 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6310 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2663 2670 3078 3302 4970 1239 Size: 1424 7 408 224 1668 2579 Fragments arranged by size: 2579 1668 1424 408 224 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4435 5300 5598 4435 Size: 865 298 5147 Fragments arranged by size: 5147 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2471 4973 882 Size: 617 972 2502 2219 Fragments arranged by size: 2502 2219 972 617 BsaAI yAC'GTr Cuts at: 493 1204 4101 493 Size: 711 2897 2702 Fragments arranged by size: 2897 2702 711 BsaBI GATnn'nnATC Cuts at: 2177 6304 2177 Size: 4127 2183 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4117 4499 5134 275 Size: 53 83 186 936 2584 382 635 1451 Fragments arranged by size: 2584 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2758 4743 5141 5415 5562 5973 2758 Size: 1985 398 274 147 411 3095 Fragments arranged by size: 3095 1985 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3405 3435 1178 Size: 30 165 30 2002 30 4053 Fragments arranged by size: 4053 2002 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3266 1186 Size: 2080 4230 BseYI C'CCAG_C Cuts at: 2188 5513 6009 2188 Size: 3325 496 2489 Fragments arranged by size: 3325 2489 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2562 1163 Size: 573 826 4911 Fragments arranged by size: 4911 826 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3760 4521 5549 1540 Size: 275 186 1759 761 1028 2301 Fragments arranged by size: 2301 1759 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4371 4456 5527 4371 Size: 85 1071 5154 Fragments arranged by size: 5154 1071 85 BsmI GAATG_Cn' Cuts at: 3821 3914 3821 Size: 93 6217 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 2695 3629 4371 4456 5527 1391 Size: 399 251 654 934 742 85 1071 2174 Fragments arranged by size: 2174 1071 934 742 654 399 251 85 BspEI T'CCGG_A Cuts at: 2758 5973 2758 Size: 3215 3095 BspHI T'CATG_A Cuts at: 2466 4199 5929 2466 Size: 1733 1730 2847 Fragments arranged by size: 2847 1733 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2041 2302 3589 3665 844 Size: 1197 261 1287 76 3489 Fragments arranged by size: 3489 1287 1197 261 76 BsrBI CCG'CTC Cuts at: 1394 1823 4197 1394 Size: 429 2374 3507 Fragments arranged by size: 3507 2374 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2250 2547 4805 4979 65 Size: 1183 1002 297 2258 174 1396 Fragments arranged by size: 2258 1396 1183 1002 297 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3458 4954 5141 1700 Size: 110 1648 1496 187 2869 Fragments arranged by size: 2869 1648 1496 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6310 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6310 BssSI C'ACGA_G Cuts at: 4370 5382 4370 Size: 1012 5298 BstAPI GCAn_nnn'nTGC Cuts at: 794 2252 2317 2549 794 Size: 1458 65 232 4555 Fragments arranged by size: 4555 1458 232 65 BstBI TT'CG_AA Cuts at: 5114 5114 Size: 6310 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6310 BstXI CCAn_nnnn'nTGG Cuts at: 2503 2602 2687 2921 2503 Size: 99 85 234 5892 Fragments arranged by size: 5892 234 99 85 BstYI r'GATC_y Cuts at: 1972 2043 2786 3640 4007 4392 4409 5861 5947 Size: 71 743 854 367 385 17 1452 86 Cuts at: 5947 5959 6305 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 854 743 385 367 346 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 2220 2220 Size: 6310 BtgI C'CryG_G Cuts at: 513 1065 2004 2595 2601 2719 2869 3600 513 Size: 552 939 591 6 118 150 731 3223 Fragments arranged by size: 3223 939 731 591 552 150 118 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5571 Fragments arranged by size: 5571 683 56 BtsI GCAGTG_nn' Cuts at: 2037 3369 3826 4620 4640 2037 Size: 1332 457 794 20 3707 Fragments arranged by size: 3707 1332 794 457 20 ClaI AT'CG_AT Cuts at: 1554 2175 1554 Size: 621 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6275 DraI TTT'AAA Cuts at: 3614 3614 Size: 6310 DraIII CAC_nnn'GTG Cuts at: 3394 3394 Size: 6310 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5317 809 Size: 507 4001 1802 Fragments arranged by size: 4001 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2598 3757 4646 Size: 54 1425 50 275 18 768 1159 889 Cuts at: 4646 6275 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1159 889 768 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3757 1537 Size: 275 1945 4090 Fragments arranged by size: 4090 1945 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2110 2135 4240 1353 Size: 757 25 2105 3423 Fragments arranged by size: 3423 2105 757 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4897 5271 5417 387 Size: 1064 138 3308 374 146 1280 Fragments arranged by size: 3308 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2094 2277 2414 2427 2663 2670 3078 Size: 393 462 183 137 13 236 7 408 Cuts at: 3078 3302 3460 4373 4970 5757 6025 1239 Size: 224 158 913 597 787 268 1524 Fragments arranged by size: 1524 913 787 597 462 408 393 268 236 224 183 158 137 13 7 EcoNI CCTnn'n_nnAGG Cuts at: 3475 3475 Size: 6310 EcoO109I rG'GnC_Cy Cuts at: 2215 2215 Size: 6310 EcoRI G'AATT_C Cuts at: 3619 3997 3619 Size: 378 5932 EcoRV GAT'ATC Cuts at: 2179 2179 Size: 6310 FspI TGC'GCA Cuts at: 4816 4816 Size: 6310 HaeII r_GCGC'y Cuts at: 1678 2015 2953 5457 1678 Size: 337 938 2504 2531 Fragments arranged by size: 2531 2504 938 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2028 2060 2793 2807 2825 Size: 32 135 32 456 32 733 14 18 Cuts at: 2825 2839 3129 3161 3195 3227 3247 3279 3405 Size: 14 290 32 34 32 20 32 126 Cuts at: 3405 3437 4951 4983 5025 5057 1373 Size: 32 1514 32 42 32 2626 Fragments arranged by size: 2626 1514 733 456 290 135 126 42 34 32 32 32 32 32 32 32 32 32 20 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 3654 3900 669 Size: 988 281 1716 246 3079 Fragments arranged by size: 3079 1716 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6310 HpaI GTT'AAC Cuts at: 3900 3900 Size: 6310 KpnI G_GTAC'C Cuts at: 3635 3635 Size: 6310 MfeI C'AATT_G Cuts at: 3909 3909 Size: 6310 MluI A'CGCG_T Cuts at: 6186 6186 Size: 6310 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2814 3181 3382 5424 5608 1305 Size: 1509 367 201 2042 184 2007 Fragments arranged by size: 2042 2007 1509 367 201 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2600 10 Size: 54 1425 343 768 3720 Fragments arranged by size: 3720 1425 768 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2600 3431 4268 4627 4786 518 Size: 648 1434 831 837 359 159 2042 Fragments arranged by size: 2042 1434 837 831 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2917 3726 4403 5551 5796 1606 Size: 1311 809 677 1148 245 2120 Fragments arranged by size: 2120 1311 1148 809 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2595 2601 2719 2869 3600 513 Size: 552 939 591 6 118 150 731 3223 Fragments arranged by size: 3223 939 731 591 552 150 118 6 NdeI CA'TA_TG Cuts at: 387 3331 387 Size: 2944 3366 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6310 NotI GC'GGCC_GC Cuts at: 3757 3757 Size: 6310 NspI r_CATG'y Cuts at: 1132 1229 2447 3023 3668 5213 1132 Size: 97 1218 576 645 1545 2229 Fragments arranged by size: 2229 1545 1218 645 576 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6310 PciI A'CATG_T Cuts at: 5209 5209 Size: 6310 PflMI CCAn_nnn'nTGG Cuts at: 1108 2997 3394 1108 Size: 1889 397 4024 Fragments arranged by size: 4024 1889 397 PfoI T'CCnGG_A Cuts at: 2679 2679 Size: 6310 PmeI GTTT'AAAC Cuts at: 3614 3614 Size: 6310 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4379 4411 5892 5924 4379 Size: 32 1481 32 4765 Fragments arranged by size: 4765 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2215 2215 Size: 6310 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6310 PsiI TTA'TAA Cuts at: 3880 3880 Size: 6310 PstI C_TGCA'G Cuts at: 830 2052 3662 830 Size: 1222 1610 3478 Fragments arranged by size: 3478 1610 1222 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6310 PvuII CAG'CTG Cuts at: 2917 2917 Size: 6310 SalI G'TCGA_C Cuts at: 1936 3652 1936 Size: 1716 4594 SanDI GG'GwC_CC Cuts at: 2215 2215 Size: 6310 SapI GCTCTTCn'nnn_ Cuts at: 2135 2135 Size: 6310 SbfI CC_TGCA'GG Cuts at: 2052 3662 2052 Size: 1610 4700 ScaI AGT'ACT Cuts at: 4558 6270 4558 Size: 1712 4598 SexAI A'CCwGG_T Cuts at: 2081 2081 Size: 6310 SfcI C'TryA_G Cuts at: 826 1045 2048 2449 3658 4793 5474 5665 826 Size: 219 1003 401 1209 1135 681 191 1471 Fragments arranged by size: 1471 1209 1135 1003 681 401 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6310 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6310 SmaI CCC'GGG Cuts at: 1872 3637 1872 Size: 1765 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4414 5315 5577 820 Size: 197 926 2471 901 262 1553 Fragments arranged by size: 2471 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4101 493 Size: 3608 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6310 SphI G_CATG'C Cuts at: 1132 2447 3023 3668 1132 Size: 1315 576 645 3774 Fragments arranged by size: 3774 1315 645 576 SspI AAT'ATT Cuts at: 5 52 4234 5 Size: 47 4182 2081 Fragments arranged by size: 4182 2081 47 StuI AGG'CCT Cuts at: 1517 2427 1517 Size: 910 5400 StyI C'CwwG_G Cuts at: 513 1065 2004 2061 2229 2595 2601 2719 2869 Size: 552 939 57 168 366 6 118 150 Cuts at: 2869 3600 3749 513 Size: 731 149 3074 Fragments arranged by size: 3074 939 731 552 366 168 150 149 118 57 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4337 4354 4507 4692 5137 325 Size: 1255 2757 17 153 185 445 1498 Fragments arranged by size: 2757 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4556 6268 96 Size: 274 80 33 51 1443 2579 1712 138 Fragments arranged by size: 2579 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6270 6302 6270 Size: 32 6278 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6310 XbaI T'CTAG_A Cuts at: 3646 3646 Size: 6310 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2236 2236 Size: 6310 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6310 XmaI C'CCGG_G Cuts at: 1870 3635 1870 Size: 1765 4545 XmnI GAAnn'nnTTC Cuts at: 4439 4439 Size: 6310 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4118 276 Size: 53 83 186 936 2584 2468 Fragments arranged by size: 2584 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI