(Linear) (Six-base) MAPSORT of: KIEE6402.seq Check: 8628 from: 1 to: 1605 KIEE6402.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1584 1605 Size: 1584 21 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 89 272 409 422 1455 1605 Size: 89 183 137 13 1033 150 Fragments arranged by size: 1033 183 150 137 89 13 AfeI AGC'GCT Cuts at: 0 946 1605 Size: 946 659 ApoI r'AATT_y Cuts at: 0 139 735 1605 Size: 139 596 870 Fragments arranged by size: 870 596 139 BamHI G'GATC_C Cuts at: 0 38 781 1605 Size: 38 743 824 Fragments arranged by size: 824 743 38 BanI G'GyrC_C Cuts at: 0 685 1605 Size: 685 920 BanII G_rGCy'C Cuts at: 0 36 1605 Size: 36 1569 BbsI GAAGACnn'nnnn_ Cuts at: 0 391 1605 Size: 391 1214 BclI T'GATC_A Cuts at: 0 1073 1605 Size: 1073 532 BglI GCCn_nnn'nGGC Cuts at: 0 226 1605 Size: 226 1379 Bme1580I G_kGCm'C Cuts at: 0 690 1605 Size: 690 915 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 658 665 1073 1297 1605 Size: 658 7 408 224 308 Fragments arranged by size: 658 408 308 224 7 BsaI GGTCTCn'nnnn_ Cuts at: 0 466 1605 Size: 466 1139 BsaBI GATnn'nnATC Cuts at: 0 172 1605 Size: 172 1433 BsaWI w'CCGG_w Cuts at: 0 753 1605 Size: 753 852 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1400 1430 1605 Size: 1400 30 175 Fragments arranged by size: 1400 175 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1261 1605 Size: 1261 344 BseYI C'CCAG_C Cuts at: 0 183 1605 Size: 183 1422 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 557 1605 Size: 557 1048 Bsp1286I G_dGCh'C Cuts at: 0 36 690 1605 Size: 36 654 915 Fragments arranged by size: 915 654 36 BspEI T'CCGG_A Cuts at: 0 753 1605 Size: 753 852 BspHI T'CATG_A Cuts at: 0 461 1605 Size: 461 1144 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 36 297 1584 1605 Size: 36 261 1287 21 Fragments arranged by size: 1287 261 36 21 BsrDI GCAATG_nn' Cuts at: 0 245 542 1605 Size: 245 297 1063 Fragments arranged by size: 1063 297 245 BsrFI r'CCGG_y Cuts at: 0 1453 1605 Size: 1453 152 BstAPI GCAn_nnn'nTGC Cuts at: 0 247 312 544 1605 Size: 247 65 232 1061 Fragments arranged by size: 1061 247 232 65 BstXI CCAn_nnnn'nTGG Cuts at: 0 498 597 682 916 1605 Size: 498 99 85 234 689 Fragments arranged by size: 689 498 234 99 85 BstYI r'GATC_y Cuts at: 0 38 781 1605 Size: 38 743 824 Fragments arranged by size: 824 743 38 Bsu36I CC'TnA_GG Cuts at: 0 215 1605 Size: 215 1390 BtgI C'CryG_G Cuts at: 0 590 596 714 864 1595 1605 Size: 590 6 118 150 731 10 Fragments arranged by size: 731 590 150 118 10 6 BtsI GCAGTG_nn' Cuts at: 0 32 1364 1605 Size: 32 1332 241 Fragments arranged by size: 1332 241 32 ClaI AT'CG_AT Cuts at: 0 170 1605 Size: 170 1435 DraIII CAC_nnn'GTG Cuts at: 0 1389 1605 Size: 1389 216 EaeI y'GGCC_r Cuts at: 0 593 1605 Size: 593 1012 EarI CTCTTCn'nnn_ Cuts at: 0 105 130 1605 Size: 105 25 1475 Fragments arranged by size: 1475 105 25 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 89 272 409 422 658 665 1073 1297 Size: 89 183 137 13 236 7 408 224 Cuts at: 1297 1455 1605 Size: 158 150 Fragments arranged by size: 408 236 224 183 158 150 137 89 13 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 1470 1605 Size: 1470 135 EcoO109I rG'GnC_Cy Cuts at: 0 210 1605 Size: 210 1395 EcoRV GAT'ATC Cuts at: 0 174 1605 Size: 174 1431 HaeII r_GCGC'y Cuts at: 0 10 948 1605 Size: 10 938 657 Fragments arranged by size: 938 657 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 23 55 788 802 820 834 1124 1156 Size: 23 32 733 14 18 14 290 32 Cuts at: 1156 1190 1222 1242 1274 1400 1432 1605 Size: 34 32 20 32 126 32 173 Fragments arranged by size: 733 290 173 126 34 32 32 32 32 32 23 20 18 14 14 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 809 1176 1377 1605 Size: 809 367 201 228 Fragments arranged by size: 809 367 228 201 MscI TGG'CCA Cuts at: 0 595 1605 Size: 595 1010 MslI CAynn'nnrTG Cuts at: 0 595 1426 1605 Size: 595 831 179 Fragments arranged by size: 831 595 179 MspA1I CmG'CkG Cuts at: 0 912 1605 Size: 912 693 NcoI C'CATG_G Cuts at: 0 590 596 714 864 1595 1605 Size: 590 6 118 150 731 10 Fragments arranged by size: 731 590 150 118 10 6 NdeI CA'TA_TG Cuts at: 0 1326 1605 Size: 1326 279 NspI r_CATG'y Cuts at: 0 442 1018 1605 Size: 442 576 587 Fragments arranged by size: 587 576 442 PflMI CCAn_nnn'nTGG Cuts at: 0 992 1389 1605 Size: 992 397 216 Fragments arranged by size: 992 397 216 PfoI T'CCnGG_A Cuts at: 0 674 1605 Size: 674 931 PpuMI rG'GwC_Cy Cuts at: 0 210 1605 Size: 210 1395 PstI C_TGCA'G Cuts at: 0 47 1605 Size: 47 1558 PvuII CAG'CTG Cuts at: 0 912 1605 Size: 912 693 SanDI GG'GwC_CC Cuts at: 0 210 1605 Size: 210 1395 SapI GCTCTTCn'nnn_ Cuts at: 0 130 1605 Size: 130 1475 SbfI CC_TGCA'GG Cuts at: 0 47 1605 Size: 47 1558 SexAI A'CCwGG_T Cuts at: 0 76 1605 Size: 76 1529 SfcI C'TryA_G Cuts at: 0 43 444 1605 Size: 43 401 1161 Fragments arranged by size: 1161 401 43 SphI G_CATG'C Cuts at: 0 442 1018 1605 Size: 442 576 587 Fragments arranged by size: 587 576 442 StuI AGG'CCT Cuts at: 0 422 1605 Size: 422 1183 StyI C'CwwG_G Cuts at: 0 56 224 590 596 714 864 1595 1605 Size: 56 168 366 6 118 150 731 10 Fragments arranged by size: 731 366 168 150 118 56 10 6 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 231 1605 Size: 231 1374 Enzymes that do cut: AarI AcuI AfeI ApoI BamHI BanI BanII BbsI BclI BglI Bme1580I BpmI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I MmeI MscI MslI MspA1I NcoI NdeI NspI PflMI PfoI PpuMI PstI PvuII SanDI SapI SbfI SexAI SfcI SphI StuI StyI XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI CspCI DraI DrdI EagI EciI EcoICRI EcoRI FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI