(Circular) (Six-base) MAPSORT of: pFN21AB6112.seq Check: 8413 from: 1 to: 6562 pFN21AB6112 6562 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3432 4372 278 Size: 53 83 186 936 1896 940 2468 Fragments arranged by size: 2468 1896 940 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3447 3905 1937 Size: 1510 458 4594 Fragments arranged by size: 4594 1510 458 Acc65I G'GTAC_C Cuts at: 3883 3883 Size: 6562 AclI AA'CG_TT Cuts at: 1563 3387 4689 5062 1563 Size: 1824 1302 373 3063 Fragments arranged by size: 3063 1824 1302 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2636 2903 2969 3389 3648 3755 4625 6009 Size: 1004 267 66 420 259 107 870 1384 Cuts at: 6009 6277 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1004 870 420 268 267 259 107 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6365 AflIII A'CryG_T Cuts at: 1203 2047 2850 3377 5461 6438 1203 Size: 844 803 527 2084 977 1327 Fragments arranged by size: 2084 1327 977 844 803 527 AgeI A'CCGG_T Cuts at: 5393 5393 Size: 6562 AhdI GACnn_n'nnGTC Cuts at: 3517 5291 3517 Size: 1774 4788 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6562 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6562 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3607 3639 3607 Size: 32 6530 AlwNI CAG_nnn'CTG Cuts at: 2757 4305 5877 2757 Size: 1548 1572 3442 Fragments arranged by size: 3442 1572 1548 ApaI G_GGCC'C Cuts at: 2482 2482 Size: 6562 ApaLI G'TGCA_C Cuts at: 4619 5775 4619 Size: 1156 5406 ApoI r'AATT_y Cuts at: 1493 3871 4097 4249 6358 1493 Size: 2378 226 152 2109 1697 Fragments arranged by size: 2378 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5116 160 Size: 872 4084 1606 Fragments arranged by size: 4084 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 AvaI C'yCGr_G Cuts at: 1870 1943 2208 3522 3749 3887 1870 Size: 73 265 1314 227 138 4545 Fragments arranged by size: 4545 1314 265 227 138 73 BamHI G'GATC_C Cuts at: 3892 4259 3892 Size: 367 6195 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2330 2872 3682 3883 5338 Size: 325 217 627 543 542 810 201 1455 Cuts at: 5338 618 Size: 1842 Fragments arranged by size: 1842 1455 810 627 543 542 325 217 201 BanII G_rGCy'C Cuts at: 1391 2308 2482 2964 3222 3881 1391 Size: 917 174 482 258 659 4072 Fragments arranged by size: 4072 917 659 482 258 174 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2142 2502 3490 928 Size: 971 243 360 988 4000 Fragments arranged by size: 4000 988 971 360 243 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3730 3764 4753 4787 1903 Size: 34 1793 34 989 34 3678 Fragments arranged by size: 3678 1793 989 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4454 5664 4454 Size: 1210 5352 BclI T'GATC_A Cuts at: 1548 2646 1548 Size: 1098 5464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3060 5173 136 Size: 107 122 71 631 605 1388 2113 1525 Fragments arranged by size: 2113 1525 1388 631 605 122 107 71 BglII A'GATC_T Cuts at: 3797 6557 3797 Size: 2760 3802 BlpI GC'TnA_GC Cuts at: 3298 3979 3298 Size: 681 5881 Bme1580I G_kGCm'C Cuts at: 1790 2442 2482 3687 4623 5779 1790 Size: 652 40 1205 936 1156 2573 Fragments arranged by size: 2573 1205 1156 936 652 40 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6562 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2476 3727 3793 Size: 416 53 83 399 24 1059 1251 66 Cuts at: 3793 4293 5251 442 Size: 500 958 1753 Fragments arranged by size: 1753 1251 1059 958 500 416 399 83 66 53 24 BmtI G_CTAG'C Cuts at: 1056 3573 1056 Size: 2517 4045 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2295 3225 3658 3681 5222 1239 Size: 1056 930 433 23 1541 2579 Fragments arranged by size: 2579 1541 1056 930 433 23 Bpu10I CC'TnA_GC Cuts at: 2411 2799 3257 2411 Size: 388 458 5716 Fragments arranged by size: 5716 458 388 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2180 2839 3806 4687 5552 5850 2180 Size: 659 967 881 865 298 2892 Fragments arranged by size: 2892 967 881 865 659 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2994 3201 5225 882 Size: 617 1495 207 2024 2219 Fragments arranged by size: 2219 2024 1495 617 207 BsaAI yAC'GTr Cuts at: 493 1204 4353 493 Size: 711 3149 2702 Fragments arranged by size: 3149 2702 711 BsaBI GATnn'nnATC Cuts at: 2271 2567 6556 2271 Size: 296 3989 2277 Fragments arranged by size: 3989 2277 296 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2499 3429 4369 4751 Size: 53 83 186 936 966 930 940 382 Cuts at: 4751 5386 275 Size: 635 1451 Fragments arranged by size: 1451 966 940 936 930 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2091 4995 5393 5667 5814 6225 2091 Size: 2904 398 274 147 411 2428 Fragments arranged by size: 2904 2428 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2654 2684 3204 3234 3679 Size: 30 165 30 1251 30 520 30 445 Cuts at: 3679 3709 1178 Size: 30 4031 Fragments arranged by size: 4031 1251 520 445 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2298 2334 1186 Size: 1112 36 5414 Fragments arranged by size: 5414 1112 36 BseYI C'CCAG_C Cuts at: 3420 5765 6261 3420 Size: 2345 496 3721 Fragments arranged by size: 3721 2345 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2452 3642 1163 Size: 573 716 1190 4083 Fragments arranged by size: 4083 1190 716 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4012 4773 5801 1540 Size: 275 186 2011 761 1028 2301 Fragments arranged by size: 2301 2011 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2308 2964 4623 4708 5779 2308 Size: 656 1659 85 1071 3091 Fragments arranged by size: 3091 1659 1071 656 85 BsmI GAATG_Cn' Cuts at: 2277 3261 3622 3722 3851 4073 4166 2277 Size: 984 361 100 129 222 93 4673 Fragments arranged by size: 4673 984 361 222 129 100 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3386 3386 Size: 6562 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2308 2442 2482 2964 3222 3687 3881 Size: 399 518 134 40 482 258 465 194 Cuts at: 3881 4623 4708 5779 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 518 482 465 399 258 194 134 85 40 BspEI T'CCGG_A Cuts at: 6225 6225 Size: 6562 BspHI T'CATG_A Cuts at: 2649 3855 4451 6181 2649 Size: 1206 596 1730 3030 Fragments arranged by size: 3030 1730 1206 596 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3917 844 Size: 3073 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2923 4449 1394 Size: 429 1100 1526 3507 Fragments arranged by size: 3507 1526 1100 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2190 3470 3555 5057 5231 65 Size: 1183 942 1280 85 1502 174 1396 Fragments arranged by size: 1502 1396 1280 1183 942 174 85 BsrFI r'CCGG_y Cuts at: 1700 1810 5206 5393 1700 Size: 110 3396 187 2869 Fragments arranged by size: 3396 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2835 96 Size: 2739 3823 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6562 BssSI C'ACGA_G Cuts at: 4622 5634 4622 Size: 1012 5550 BstAPI GCAn_nnn'nTGC Cuts at: 794 3175 794 Size: 2381 4181 BstBI TT'CG_AA Cuts at: 5366 5366 Size: 6562 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6562 BstYI r'GATC_y Cuts at: 1972 2886 3633 3797 3837 3892 4259 4644 4661 Size: 914 747 164 40 55 367 385 17 Cuts at: 4661 6113 6199 6211 6557 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 914 747 385 367 346 164 86 55 40 17 12 Bsu36I CC'TnA_GG Cuts at: 2774 2774 Size: 6562 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5071 Fragments arranged by size: 5071 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5823 Fragments arranged by size: 5823 683 56 BtsI GCAGTG_nn' Cuts at: 2440 4078 4872 4892 2440 Size: 1638 794 20 4110 Fragments arranged by size: 4110 1638 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6562 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6527 DraI TTT'AAA Cuts at: 3866 3866 Size: 6562 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2244 5569 809 Size: 507 928 3325 1802 Fragments arranged by size: 3325 1802 928 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3061 4009 4898 Size: 54 1425 50 275 18 1231 948 889 Cuts at: 4898 6527 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1231 948 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4009 1537 Size: 275 2197 4090 Fragments arranged by size: 4090 2197 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2122 2587 2781 2952 3149 3624 4492 1353 Size: 769 465 194 171 197 475 868 3423 Fragments arranged by size: 3423 868 769 475 465 197 194 171 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5149 5523 5669 387 Size: 1064 138 435 3125 374 146 1280 Fragments arranged by size: 3125 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2306 2962 2306 Size: 656 5906 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2295 2636 2903 2969 3225 3389 3648 Size: 393 663 341 267 66 256 164 259 Cuts at: 3648 3658 3681 3755 4625 5222 6009 6277 1239 Size: 10 23 74 870 597 787 268 1524 Fragments arranged by size: 1524 870 787 663 597 393 341 268 267 259 256 164 74 66 23 10 EcoO109I rG'GnC_Cy Cuts at: 2084 2346 3243 2084 Size: 262 897 5403 Fragments arranged by size: 5403 897 262 EcoRI G'AATT_C Cuts at: 3871 4249 3871 Size: 378 6184 EcoRV GAT'ATC Cuts at: 2565 2565 Size: 6562 FspI TGC'GCA Cuts at: 5068 5068 Size: 6562 HaeII r_GCGC'y Cuts at: 1678 3024 3459 5709 1678 Size: 1346 435 2250 2531 Fragments arranged by size: 2531 2250 1346 435 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2006 2038 2333 2365 2530 Size: 32 135 32 434 32 295 32 165 Cuts at: 2530 2562 2792 2824 2837 2869 2963 2995 3204 Size: 32 230 32 13 32 94 32 209 Cuts at: 3204 3236 3503 3535 5203 5235 5277 5309 1373 Size: 32 267 32 1668 32 42 32 2626 Fragments arranged by size: 2626 1668 434 295 267 230 209 165 135 94 42 32 32 32 32 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 2530 3906 4152 669 Size: 988 281 592 1376 246 3079 Fragments arranged by size: 3079 1376 988 592 281 246 HindIII A'AGCT_T Cuts at: 748 3335 748 Size: 2587 3975 HpaI GTT'AAC Cuts at: 4152 4152 Size: 6562 KpnI G_GTAC'C Cuts at: 3887 3887 Size: 6562 MfeI C'AATT_G Cuts at: 3081 4161 3081 Size: 1080 5482 MluI A'CGCG_T Cuts at: 2047 3377 6438 2047 Size: 1330 3061 2171 Fragments arranged by size: 3061 2171 1330 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5676 5860 1305 Size: 4371 184 2007 Fragments arranged by size: 4371 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3063 10 Size: 54 1425 343 1231 3509 Fragments arranged by size: 3509 1425 1231 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2040 3475 4520 4879 5038 518 Size: 648 874 1435 1045 359 159 2042 Fragments arranged by size: 2042 1435 1045 874 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3978 4655 5803 6048 1606 Size: 2372 677 1148 245 2120 Fragments arranged by size: 2372 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5071 Fragments arranged by size: 5071 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6562 NheI G'CTAG_C Cuts at: 1052 3569 1052 Size: 2517 4045 NotI GC'GGCC_GC Cuts at: 4009 4009 Size: 6562 NruI TCG'CGA Cuts at: 3030 3030 Size: 6562 NspI r_CATG'y Cuts at: 1132 1229 2854 3920 5465 1132 Size: 97 1625 1066 1545 2229 Fragments arranged by size: 2229 1625 1545 1066 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6562 PciI A'CATG_T Cuts at: 2850 5461 2850 Size: 2611 3951 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6562 PfoI T'CCnGG_A Cuts at: 3222 3222 Size: 6562 PmeI GTTT'AAAC Cuts at: 3866 3866 Size: 6562 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4631 4663 6144 6176 4631 Size: 32 1481 32 5017 Fragments arranged by size: 5017 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2084 2346 2084 Size: 262 6300 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6562 PsiI TTA'TAA Cuts at: 4132 4132 Size: 6562 PspOMI G'GGCC_C Cuts at: 2478 2478 Size: 6562 PspXI vC'TCGA_Gb Cuts at: 3749 3749 Size: 6562 PstI C_TGCA'G Cuts at: 830 3914 830 Size: 3084 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6562 SacI G_AGCT'C Cuts at: 2308 2964 2308 Size: 656 5906 SalI G'TCGA_C Cuts at: 1936 3904 1936 Size: 1968 4594 SanDI GG'GwC_CC Cuts at: 2084 2084 Size: 6562 SapI GCTCTTCn'nnn_ Cuts at: 2952 2952 Size: 6562 SbfI CC_TGCA'GG Cuts at: 3914 3914 Size: 6562 ScaI AGT'ACT Cuts at: 2506 3185 4810 6522 2506 Size: 679 1625 1712 2546 Fragments arranged by size: 2546 1712 1625 679 SfcI C'TryA_G Cuts at: 826 1045 3910 5045 5726 5917 826 Size: 219 2865 1135 681 191 1471 Fragments arranged by size: 2865 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6562 SmaI CCC'GGG Cuts at: 1872 3524 3889 1872 Size: 1652 365 4545 Fragments arranged by size: 4545 1652 365 SmlI C'TyrA_G Cuts at: 820 1017 1943 2159 2818 3749 3821 4666 5567 Size: 197 926 216 659 931 72 845 901 Cuts at: 5567 5829 820 Size: 262 1553 Fragments arranged by size: 1553 931 926 901 845 659 262 216 197 72 SnaBI TAC'GTA Cuts at: 493 4353 493 Size: 3860 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6562 SphI G_CATG'C Cuts at: 1132 3920 1132 Size: 2788 3774 SspI AAT'ATT Cuts at: 5 52 4486 5 Size: 47 4434 2081 Fragments arranged by size: 4434 2081 47 StuI AGG'CCT Cuts at: 1517 2697 3809 1517 Size: 1180 1112 4270 Fragments arranged by size: 4270 1180 1112 StyI C'CwwG_G Cuts at: 513 1065 2004 3828 4001 513 Size: 552 939 1824 173 3074 Fragments arranged by size: 3074 1824 939 552 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2136 2890 3225 3676 4589 4606 4759 Size: 1255 556 754 335 451 913 17 153 Cuts at: 4759 4944 5389 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 913 754 556 451 445 335 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2504 2835 3183 Size: 274 80 33 51 1443 527 331 348 Cuts at: 3183 4808 6520 96 Size: 1625 1712 138 Fragments arranged by size: 1712 1625 1443 527 348 331 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2089 2121 3679 3711 6522 6554 2089 Size: 32 1558 32 2811 32 2097 Fragments arranged by size: 2811 2097 1558 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2738 1316 Size: 1422 5140 XbaI T'CTAG_A Cuts at: 3898 3898 Size: 6562 XhoI C'TCGA_G Cuts at: 1943 3749 1943 Size: 1806 4756 XmaI C'CCGG_G Cuts at: 1870 3522 3887 1870 Size: 1652 365 4545 Fragments arranged by size: 4545 1652 365 XmnI GAAnn'nnTTC Cuts at: 2360 3036 4691 2360 Size: 676 1655 4231 Fragments arranged by size: 4231 1655 676 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3430 4370 276 Size: 53 83 186 936 1896 940 2468 Fragments arranged by size: 2468 1896 940 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI