################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbvCI BcgI BclI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaBI BsaI BsaWI BseAI BsiWI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KpnI MaeI MaeIII MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PleI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AspHI 637 GAGCTC G[AT]GC[AT]^C AspI 562 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 657 TGGCCA TGG^CCA BbeI 271 GGCGCC GGCGC^C BbrPI 214 CACGTG CAC^GTG BciVI 375 GGATAC GTATCC(6/5) BcnI 522 CCGGG CC^[GC]GG BglII 368 AGATCT A^GATCT BsaAI 214 CACGTG [CT]AC^GT[GA] BseRI 229 GAGGAG GAGGAG(10/8) BsiEI 519 CGGCCG CG[GA][CT]^CG BsiHKAI 637 GAGCTC G[AT]GC[AT]^C BsmAI 448 GAGAC GTCTC(1/5) BsmBI 448 GAGACG CGTCTC(1/5) BsmI 252 GAATGC GAATGC(1/-1) BsrFI 17 GCCGGC [GA]^CCGG[CT] BssSI 160 CACGAG CACGAG(-5/-1) BstXI 496 CCAAACACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 368 AGATCT [GA]^GATC[CT] Cfr10I 17 GCCGGC [GA]^CCGG[CT] DdeI 365 CTGAG C^T[ACGT]AG EagI 519 CGGCCG C^GGCCG EclXI 519 CGGCCG C^GGCCG Eco52I 519 CGGCCG C^GGCCG EcoT14I 329 CCTTGG C^C[AT][AT]GG HgaI 527 GCGTC GACGC(5/10) KasI 271 GGCGCC G^GCGCC KspI 339 CCGCGG CCGC^GG MaeII 215 ACGT A^CGT MflI 368 AGATCT [GA]^GATC[CT] MluNI 657 TGGCCA TGG^CCA MscI 657 TGGCCA TGG^CCA MslI 501 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 17 GCCGGC GCC^GGC NarI 271 GGCGCC GG^CGCC NciI 522 CCGGG CC^[GC]GG NgoMIV 17 GCCGGC G^CCGGC PflFI 562 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 159 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 214 CACGTG CAC^GTG PmlI 214 CACGTG CAC^GTG PpuMI 662 AGGACCC [GA]G^G[AT]CC[CT] PshAI 66 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 235 CTGCAG CTGCA^G PvuII 479 CAGCTG CAG^CTG SacI 637 GAGCTC GAGCT^C SacII 339 CCGCGG CCGC^GG SapI 315 GCTCTTC GCTCTTC(1/4) SfcI 235 CTGCAG C^T[GA][CT]AG SfoI 271 GGCGCC GGC^GCC SmlI 634 CTTGAG C^T[CT][GA]AG StyI 329 CCTTGG C^C[AT][AT]GG Tth111I 562 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 159 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 368 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 271 GGCGCC G[GA]^CG[CT]C 526 GGCGTC AvaI 348 CTCGGG C^[CT]CG[GA]G 620 CTCGGG AvaII 156 GGACC G^G[AT]CC 663 GGACC BanII 333 GGGCTC G[GA]GC[CT]^C 637 GAGCTC BsaHI 271 GGCGCC G[GA]^CG[CT]C 526 GGCGTC BsgI 202 CTGCAC GTGCAG(16/14) 286 CTGCAC BsoBI 348 CTCGGG C^[CT]CG[GA]G 620 CTCGGG BsrI 359 ACTGG ACTGG(1/-1) 402 CCAGT DpnII 183 GATC ^GATC 369 GATC DraII 623 GGGGCCT [GA]G^G[ACGT]CC[CT] 662 AGGACCC DsaI 11 CCGTGG C^C[GA][CT]GG 339 CCGCGG EcoO109I 623 GGGGCCT [GA]G^G[ACGT]CC[CT] 662 AGGACCC FauI 338 CCCGC CCCGC(4/6) 256 GCGGG HaeII 271 GGCGCC [GA]GCGC^[CT] 353 GGCGCT Hin1I 271 GGCGCC G[GA]^CG[CT]C 526 GGCGTC MboI 183 GATC ^GATC 369 GATC NdeII 183 GATC ^GATC 369 GATC NlaIII 276 CATG CATG^ 503 CATG Sau3AI 183 GATC ^GATC 369 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AccII AciI AfaI AluI BanI BbsI BbvI BmyI BpuAI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I EaeI EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MboII MnlI MspA1I MspI MvaI MvnI MwoI NlaIV RsaI Sau96I ScrFI TseI TspRI