Restriction map for
pFN21AE6321
(Linear) (Six-base) MAPSORT of: KIEE6321.seq Check: 2626 from: 1 to: 1011
KIEE6321.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
Acc65I G'GTAC_C
Cuts at: 0 638 1011
Size: 638 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 949 1011
Size: 773 176 62
Fragments arranged by size:
773 176 62
AflII C'TTAA_G
Cuts at: 0 37 1011
Size: 37 974
AlwNI CAG_nnn'CTG
Cuts at: 0 475 478 835 948 1011
Size: 475 3 357 113 63
Fragments arranged by size:
475 357 113 63 3
BanI G'GyrC_C
Cuts at: 0 334 411 562 638 1011
Size: 334 77 151 76 373
Fragments arranged by size:
373 334 151 77 76
BanII G_rGCy'C
Cuts at: 0 155 537 942 1011
Size: 155 382 405 69
Fragments arranged by size:
405 382 155 69
BbvCI CC'TCA_GC
Cuts at: 0 920 1011
Size: 920 91
Bme1580I G_kGCm'C
Cuts at: 0 303 531 1011
Size: 303 228 480
Fragments arranged by size:
480 303 228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 857 970 1011
Size: 857 113 41
Fragments arranged by size:
857 113 41
Bpu10I CC'TnA_GC
Cuts at: 0 920 941 1011
Size: 920 21 70
Fragments arranged by size:
920 70 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 916 1011
Size: 295 621 95
Fragments arranged by size:
621 295 95
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 385 937 1011
Size: 325 60 552 74
Fragments arranged by size:
552 325 74 60
BseYI C'CCAG_C
Cuts at: 0 484 1011
Size: 484 527
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 1011
Size: 848 163
BsiHKAI G_wGCw'C
Cuts at: 0 155 443 1011
Size: 155 288 568
Fragments arranged by size:
568 288 155
Bsp1286I G_dGCh'C
Cuts at: 0 155 303 443 531 537 942 1011
Size: 155 148 140 88 6 405 69
Fragments arranged by size:
405 155 148 140 88 69 6
BspHI T'CATG_A
Cuts at: 0 191 1011
Size: 191 820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 312 502 1011
Size: 312 190 509
Fragments arranged by size:
509 312 190
BsrDI GCAATG_nn'
Cuts at: 0 341 806 1011
Size: 341 465 205
Fragments arranged by size:
465 341 205
BssSI C'ACGA_G
Cuts at: 0 530 1011
Size: 530 481
BstAPI GCAn_nnn'nTGC
Cuts at: 0 475 1011
Size: 475 536
BstXI CCAn_nnnn'nTGG
Cuts at: 0 822 1011
Size: 822 189
BstYI r'GATC_y
Cuts at: 0 977 1011
Size: 977 34
BtsI GCAGTG_nn'
Cuts at: 0 241 504 1011
Size: 241 263 507
Fragments arranged by size:
507 263 241
EcoICRI GAG'CTC
Cuts at: 0 153 1011
Size: 153 858
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 857 949 970 1011
Size: 773 84 92 21 41
Fragments arranged by size:
773 92 84 41 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 307 704 1011
Size: 307 397 307
Fragments arranged by size:
397 307 307
EcoO109I rG'GnC_Cy
Cuts at: 0 787 1011
Size: 787 224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 981 1011
Size: 981 30
HincII GTy'rAC
Cuts at: 0 630 1011
Size: 630 381
HindIII A'AGCT_T
Cuts at: 0 172 1011
Size: 172 839
KpnI G_GTAC'C
Cuts at: 0 642 1011
Size: 642 369
MfeI C'AATT_G
Cuts at: 0 633 1011
Size: 633 378
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 1011
Size: 383 628
MslI CAynn'nnrTG
Cuts at: 0 190 454 820 1011
Size: 190 264 366 191
Fragments arranged by size:
366 264 191 190
MspA1I CmG'CkG
Cuts at: 0 475 751 919 1011
Size: 475 276 168 92
Fragments arranged by size:
475 276 168 92
NdeI CA'TA_TG
Cuts at: 0 876 1011
Size: 876 135
NspI r_CATG'y
Cuts at: 0 733 1011
Size: 733 278
PasI CC'CwG_GG
Cuts at: 0 134 1011
Size: 134 877
PfoI T'CCnGG_A
Cuts at: 0 47 1011
Size: 47 964
PstI C_TGCA'G
Cuts at: 0 499 995 1011
Size: 499 496 16
Fragments arranged by size:
499 496 16
PvuII CAG'CTG
Cuts at: 0 475 751 919 1011
Size: 475 276 168 92
Fragments arranged by size:
475 276 168 92
SacI G_AGCT'C
Cuts at: 0 155 1011
Size: 155 856
SfcI C'TryA_G
Cuts at: 0 495 991 1011
Size: 495 496 20
Fragments arranged by size:
496 495 20
SmlI C'TyrA_G
Cuts at: 0 37 274 895 1011
Size: 37 237 621 116
Fragments arranged by size:
621 237 116 37
SpeI A'CTAG_T
Cuts at: 0 510 1011
Size: 510 501
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 586 624 814 1011
Size: 586 38 190 197
Fragments arranged by size:
586 197 190 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 282 314 657 689 1011
Size: 282 32 343 32 322
Fragments arranged by size:
343 322 282 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 348 1011
Size: 348 663
XmnI GAAnn'nnTTC
Cuts at: 0 109 1011
Size: 109 902
Enzymes that do cut:
Acc65I AcuI AflII AlwNI BanI BanII BbvCI Bme1580I
BpmI Bpu10I BpuEI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrDI BssSI BstAPI BstXI BstYI BtsI
EcoICRI Eco57MI EcoNI EcoO109I FalI HincII HindIII KpnI
MfeI MmeI MslI MspA1I NdeI NspI PasI PfoI
PstI PvuII SacI SfcI SmlI SpeI TaqII TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HpaI
KasI MluI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI StuI StyI SwaI TatI Tth111I XbaI XhoI
XmaI ZraI