################################## # NO CUT ENZYMES ( 201 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaJI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AfaI 190 GTAC GT^AC AluI 392 AGCT AG^CT ApaI 374 GGGCCC GGGCC^C AseI 207 ATTAAT AT^TAAT AsnI 207 ATTAAT AT^TAAT AvaII 412 GGTCC G^G[AT]CC BanII 374 GGGCCC G[GA]GC[CT]^C BbrPI 58 CACGTG CAC^GTG BglI 367 GCCGACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 374 GGGCCC G[AGT]GC[ACT]^C BsaAI 58 CACGTG [CT]AC^GT[GA] BsaWI 386 ACCGGA [AT]^CCGG[AT] BsgI 10 GTGCAG GTGCAG(16/14) Bsp1286I 374 GGGCCC G[AGT]GC[ACT]^C BspDI 203 ATCGAT AT^CGAT Cac8I 75 GCTGGC GC[ACGT]^[ACGT]GC ClaI 203 ATCGAT AT^CGAT DdeI 193 CTCAG C^T[ACGT]AG DpnII 202 GATC ^GATC HapII 387 CCGG C^CGG HindIII 391 AAGCTT A^AGCTT HpaII 387 CCGG C^CGG HphI 158 TCACC GGTGA(8/7) MboI 202 GATC ^GATC MboII 63 GAAGA GAAGA(8/7) MnlI 329 GAGG CCTC(7/6) MseI 208 TTAA T^TAA MspA1I 408 CAGCGG C[AC]G^C[GT]G MspI 387 CCGG C^CGG NdeII 202 GATC ^GATC PmaCI 58 CACGTG CAC^GTG PmlI 58 CACGTG CAC^GTG PshBI 207 ATTAAT AT^TAAT RsaI 190 GTAC GT^AC Sau3AI 202 GATC ^GATC SfaNI 66 GATGC GCATC(5/9) TaqI 204 TCGA T^CGA Tru9I 208 TTAA T^TAA TthHB8I 204 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AciI 72 GCGG CCGC(-3/-1) 410 GCGG BbvI 12 GCAGC GCAGC(8/12) 69 GCTGC BsrI 145 ACTGG ACTGG(1/-1) 252 ACTGG DraII 373 GGGGCCC [GA]G^G[ACGT]CC[CT] 374 GGGCCCT EcoO109I 373 GGGGCCC [GA]G^G[ACGT]CC[CT] 374 GGGCCCT MaeII 8 ACGT A^CGT 59 ACGT MwoI 32 GCCCCTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 367 GCCGACGGGGC NlaIII 270 CATG CATG^ 436 CATG NlaIV 373 GGGGCC GG[ACGT]^[ACGT]CC 374 GGGCCC TseI 12 GCAGC G^C[AT]GC 69 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### Cfr13I Fnu4HI HaeIII ItaI MaeIII Sau96I Tsp45I TspRI