(Circular) (Six-base) MAPSORT of: pFN21AE1518.seq Check: 8196 from: 1 to: 5161 pFN21AE1518 5161 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2971 278 Size: 53 83 186 936 1435 2468 Fragments arranged by size: 2468 1435 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2504 1937 Size: 567 4594 Acc65I G'GTAC_C Cuts at: 2482 2482 Size: 5161 AclI AA'CG_TT Cuts at: 1563 3288 3661 1563 Size: 1725 373 3063 Fragments arranged by size: 3063 1725 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2032 3224 4608 4876 1632 Size: 400 1192 1384 268 1917 Fragments arranged by size: 1917 1384 1192 400 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4964 AflIII A'CryG_T Cuts at: 1203 4060 5037 1203 Size: 2857 977 1327 Fragments arranged by size: 2857 1327 977 AgeI A'CCGG_T Cuts at: 3992 3992 Size: 5161 AhdI GACnn_n'nnGTC Cuts at: 3890 3890 Size: 5161 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5161 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5161 AlwNI CAG_nnn'CTG Cuts at: 2904 4476 2904 Size: 1572 3589 ApaI G_GGCC'C Cuts at: 2383 2383 Size: 5161 ApaLI G'TGCA_C Cuts at: 3218 4374 3218 Size: 1156 4005 ApoI r'AATT_y Cuts at: 1493 2470 2696 2848 4957 1493 Size: 977 226 152 2109 1697 Fragments arranged by size: 2109 1697 977 226 152 AseI AT'TA_AT Cuts at: 160 1032 2213 3715 160 Size: 872 1181 1502 1606 Fragments arranged by size: 1606 1502 1181 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5161 AvaI C'yCGr_G Cuts at: 1870 1943 2486 1870 Size: 73 543 4545 Fragments arranged by size: 4545 543 73 BamHI G'GATC_C Cuts at: 2491 2858 2491 Size: 367 4794 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2482 3937 618 Size: 325 217 627 695 1455 1842 Fragments arranged by size: 1842 1455 695 627 325 217 BanII G_rGCy'C Cuts at: 1391 2383 2480 1391 Size: 992 97 4072 Fragments arranged by size: 4072 992 97 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4190 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3352 3386 1903 Size: 34 1415 34 3678 Fragments arranged by size: 3678 1415 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3053 4263 3053 Size: 1210 3951 BclI T'GATC_A Cuts at: 1548 1548 Size: 5161 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2378 3772 136 Size: 107 122 71 631 605 706 1394 1525 Fragments arranged by size: 1525 1394 706 631 605 122 107 71 BglII A'GATC_T Cuts at: 5156 5156 Size: 5161 BlpI GC'TnA_GC Cuts at: 2578 2578 Size: 5161 Bme1580I G_kGCm'C Cuts at: 1790 2383 3222 4378 1790 Size: 593 839 1156 2573 Fragments arranged by size: 2573 1156 839 593 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5161 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2892 3850 442 Size: 416 53 83 399 24 1475 958 1753 Fragments arranged by size: 1753 1475 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5161 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3821 1239 Size: 2582 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3286 4151 4449 3286 Size: 865 298 3998 Fragments arranged by size: 3998 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3824 882 Size: 617 2325 2219 Fragments arranged by size: 2325 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2065 2952 493 Size: 711 861 887 2702 Fragments arranged by size: 2702 887 861 711 BsaBI GATnn'nnATC Cuts at: 5155 5155 Size: 5161 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2968 3350 3985 275 Size: 53 83 186 936 1435 382 635 1451 Fragments arranged by size: 1451 1435 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2391 3594 3992 4266 4413 4824 2391 Size: 1203 398 274 147 411 2728 Fragments arranged by size: 2728 1203 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4936 Fragments arranged by size: 4936 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5161 BseYI C'CCAG_C Cuts at: 2411 4364 4860 2411 Size: 1953 496 2712 Fragments arranged by size: 2712 1953 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2036 1163 Size: 573 300 4288 Fragments arranged by size: 4288 573 300 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2611 3372 4400 1540 Size: 275 186 610 761 1028 2301 Fragments arranged by size: 2301 1028 761 610 275 186 BsiHKAI G_wGCw'C Cuts at: 3222 3307 4378 3222 Size: 85 1071 4005 Fragments arranged by size: 4005 1071 85 BsmI GAATG_Cn' Cuts at: 2672 2765 2672 Size: 93 5068 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2383 2480 3222 3307 4378 1391 Size: 399 593 97 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 593 399 97 85 BspEI T'CCGG_A Cuts at: 4824 4824 Size: 5161 BspHI T'CATG_A Cuts at: 3050 4780 3050 Size: 1730 3431 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2516 844 Size: 1672 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3048 1394 Size: 429 1225 3507 Fragments arranged by size: 3507 1225 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3656 3830 65 Size: 1183 2408 174 1396 Fragments arranged by size: 2408 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3805 3992 1700 Size: 110 1995 187 2869 Fragments arranged by size: 2869 1995 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5161 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5161 BssSI C'ACGA_G Cuts at: 3221 4233 3221 Size: 1012 4149 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5161 BstBI TT'CG_AA Cuts at: 3965 3965 Size: 5161 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5161 BstYI r'GATC_y Cuts at: 1972 2491 2858 3243 3260 4712 4798 4810 5156 Size: 519 367 385 17 1452 86 12 346 Cuts at: 5156 1972 Size: 1977 Fragments arranged by size: 1977 1452 519 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3670 Fragments arranged by size: 3670 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2018 2101 487 Size: 683 56 792 83 3547 Fragments arranged by size: 3547 792 683 83 56 BtsI GCAGTG_nn' Cuts at: 2677 3471 3491 2677 Size: 794 20 4347 Fragments arranged by size: 4347 794 20 ClaI AT'CG_AT Cuts at: 1554 2209 1554 Size: 655 4506 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5126 DraI TTT'AAA Cuts at: 2465 2465 Size: 5161 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4168 809 Size: 507 2852 1802 Fragments arranged by size: 2852 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2608 3497 5126 Size: 54 1425 50 275 18 778 889 1629 Cuts at: 5126 8 Size: 43 Fragments arranged by size: 1629 1425 889 778 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2608 1537 Size: 275 796 4090 Fragments arranged by size: 4090 796 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3091 1353 Size: 1738 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3748 4122 4268 387 Size: 1064 138 2159 374 146 1280 Fragments arranged by size: 2159 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 3224 3821 4608 4876 1239 Size: 393 400 1192 597 787 268 1524 Fragments arranged by size: 1524 1192 787 597 400 393 268 EcoO109I rG'GnC_Cy Cuts at: 2379 2380 2379 Size: 1 5160 EcoRI G'AATT_C Cuts at: 2470 2848 2470 Size: 378 4783 FspI TGC'GCA Cuts at: 3667 3667 Size: 5161 HaeII r_GCGC'y Cuts at: 1678 4308 1678 Size: 2630 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2145 2177 3802 3834 3876 Size: 32 135 32 573 32 1625 32 42 Cuts at: 3876 3908 1373 Size: 32 2626 Fragments arranged by size: 2626 1625 573 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2505 2751 669 Size: 988 281 567 246 3079 Fragments arranged by size: 3079 988 567 281 246 HindIII A'AGCT_T Cuts at: 748 2396 748 Size: 1648 3513 HpaI GTT'AAC Cuts at: 2751 2751 Size: 5161 KpnI G_GTAC'C Cuts at: 2486 2486 Size: 5161 MfeI C'AATT_G Cuts at: 2760 2760 Size: 5161 MluI A'CGCG_T Cuts at: 5037 5037 Size: 5161 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4275 4459 1305 Size: 2970 184 2007 Fragments arranged by size: 2970 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3339 Fragments arranged by size: 3339 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3119 3478 3637 518 Size: 648 1953 359 159 2042 Fragments arranged by size: 2042 1953 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2415 2577 3254 4402 4647 1606 Size: 809 162 677 1148 245 2120 Fragments arranged by size: 2120 1148 809 677 245 162 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3670 Fragments arranged by size: 3670 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5161 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5161 NotI GC'GGCC_GC Cuts at: 2608 2608 Size: 5161 NspI r_CATG'y Cuts at: 1132 1229 2519 4064 1132 Size: 97 1290 1545 2229 Fragments arranged by size: 2229 1545 1290 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5161 PciI A'CATG_T Cuts at: 4060 4060 Size: 5161 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5161 PmeI GTTT'AAAC Cuts at: 2465 2465 Size: 5161 PmlI CAC'GTG Cuts at: 2065 2065 Size: 5161 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3230 3262 4743 4775 3230 Size: 32 1481 32 3616 Fragments arranged by size: 3616 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5161 PsiI TTA'TAA Cuts at: 2731 2731 Size: 5161 PspOMI G'GGCC_C Cuts at: 2379 2379 Size: 5161 PstI C_TGCA'G Cuts at: 830 2513 830 Size: 1683 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5161 SalI G'TCGA_C Cuts at: 1936 2503 1936 Size: 567 4594 SbfI CC_TGCA'GG Cuts at: 2513 2513 Size: 5161 ScaI AGT'ACT Cuts at: 3409 5121 3409 Size: 1712 3449 SfcI C'TryA_G Cuts at: 826 1045 2509 3644 4325 4516 826 Size: 219 1464 1135 681 191 1471 Fragments arranged by size: 1471 1464 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5161 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5161 SmaI CCC'GGG Cuts at: 1872 2488 1872 Size: 616 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3265 4166 4428 820 Size: 197 926 1322 901 262 1553 Fragments arranged by size: 1553 1322 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2952 493 Size: 2459 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5161 SphI G_CATG'C Cuts at: 1132 2519 1132 Size: 1387 3774 SspI AAT'ATT Cuts at: 5 52 3085 5 Size: 47 3033 2081 Fragments arranged by size: 3033 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5161 StyI C'CwwG_G Cuts at: 513 1065 2004 2600 513 Size: 552 939 596 3074 Fragments arranged by size: 3074 939 596 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3188 3205 3358 3543 3988 325 Size: 1255 1608 17 153 185 445 1498 Fragments arranged by size: 1608 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2194 3407 5119 Size: 274 80 33 51 1443 217 1213 1712 Cuts at: 5119 96 Size: 138 Fragments arranged by size: 1712 1443 1213 274 217 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5121 5153 5121 Size: 32 5129 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5161 XbaI T'CTAG_A Cuts at: 2497 2497 Size: 5161 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5161 XmaI C'CCGG_G Cuts at: 1870 2486 1870 Size: 616 4545 XmnI GAAnn'nnTTC Cuts at: 3290 3290 Size: 5161 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2969 276 Size: 53 83 186 936 1435 2468 Fragments arranged by size: 2468 1435 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI