(Circular) (Six-base) MAPSORT of: pFN21AB5462.seq Check: 8309 from: 1 to: 5833 pFN21AB5462 5833 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2574 3643 278 Size: 53 83 186 936 1038 1069 2468 Fragments arranged by size: 2468 1069 1038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2283 3176 1937 Size: 346 893 4594 Fragments arranged by size: 4594 893 346 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3896 5280 5548 1632 Size: 2264 1384 268 1917 Fragments arranged by size: 2264 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 2727 4732 5709 1203 Size: 1524 2005 977 1327 Fragments arranged by size: 2005 1524 1327 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5833 AlwNI CAG_nnn'CTG Cuts at: 3576 5148 3576 Size: 1572 4261 ApaI G_GGCC'C Cuts at: 2779 2779 Size: 5833 ApaLI G'TGCA_C Cuts at: 3890 5046 3890 Size: 1156 4677 ApoI r'AATT_y Cuts at: 1493 3142 3368 3520 5629 1493 Size: 1649 226 152 2109 1697 Fragments arranged by size: 2109 1697 1649 226 152 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 2353 2780 3158 1870 Size: 73 410 427 378 4545 Fragments arranged by size: 4545 427 410 378 73 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3154 4609 618 Size: 325 217 627 1367 1455 1842 Fragments arranged by size: 1842 1455 1367 627 325 217 BanII G_rGCy'C Cuts at: 1391 2158 2779 2829 3152 1391 Size: 767 621 50 323 4072 Fragments arranged by size: 4072 767 621 323 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4862 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2119 2153 3112 3146 4024 4058 1903 Size: 34 182 34 959 34 878 34 3678 Fragments arranged by size: 3678 959 878 182 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 1548 Size: 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4444 136 Size: 107 122 71 631 605 2772 1525 Fragments arranged by size: 2772 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2991 5828 2991 Size: 2837 2996 BlpI GC'TnA_GC Cuts at: 3250 3250 Size: 5833 Bme1580I G_kGCm'C Cuts at: 1790 2511 2779 3894 5050 1790 Size: 721 268 1115 1156 2573 Fragments arranged by size: 2573 1156 1115 721 268 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3564 4522 442 Size: 416 53 83 399 24 2147 958 1753 Fragments arranged by size: 2147 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3121 3121 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2172 2180 2726 4493 1239 Size: 933 8 546 1767 2579 Fragments arranged by size: 2579 1767 933 546 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3958 4823 5121 3958 Size: 865 298 4670 Fragments arranged by size: 4670 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2079 2387 2931 4496 882 Size: 617 580 308 544 1565 2219 Fragments arranged by size: 2219 1565 617 580 544 308 BsaAI yAC'GTr Cuts at: 493 1204 3624 493 Size: 711 2420 2702 Fragments arranged by size: 2702 2420 711 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2571 3640 4022 4657 Size: 53 83 186 936 1038 1069 382 635 Cuts at: 4657 275 Size: 1451 Fragments arranged by size: 1451 1069 1038 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2646 4266 4664 4938 5085 5496 2646 Size: 1620 398 274 147 411 2983 Fragments arranged by size: 2983 1620 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 1178 Size: 30 165 30 867 30 4711 Fragments arranged by size: 4711 867 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 2321 2330 1186 Size: 1021 114 9 4689 Fragments arranged by size: 4689 1021 114 9 BseYI C'CCAG_C Cuts at: 5036 5532 5036 Size: 496 5337 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2741 2748 1163 Size: 573 1005 7 4248 Fragments arranged by size: 4248 1005 573 7 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2129 2566 3283 4044 5072 1540 Size: 275 186 128 437 717 761 1028 2301 Fragments arranged by size: 2301 1028 761 717 437 275 186 128 BsiHKAI G_wGCw'C Cuts at: 2199 2235 2331 2439 2904 3894 3979 5050 2199 Size: 36 96 108 465 990 85 1071 2982 Fragments arranged by size: 2982 1071 990 465 108 96 85 36 BsmI GAATG_Cn' Cuts at: 3344 3437 3344 Size: 93 5740 BsmBI CGTCTCn'nnnn_ Cuts at: 2578 2702 2578 Size: 124 5709 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2158 2199 2235 2331 2439 2511 2779 Size: 399 368 41 36 96 108 72 268 Cuts at: 2779 2829 2904 3152 3894 3979 5050 1391 Size: 50 75 248 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 368 268 248 108 96 85 75 72 50 41 36 BspEI T'CCGG_A Cuts at: 2646 5496 2646 Size: 2850 2983 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2880 3188 844 Size: 2036 308 3489 Fragments arranged by size: 3489 2036 308 BsrBI CCG'CTC Cuts at: 1394 1823 2347 3720 1394 Size: 429 524 1373 3507 Fragments arranged by size: 3507 1373 524 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2556 4328 4502 65 Size: 1183 1308 1772 174 1396 Fragments arranged by size: 1772 1396 1308 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2407 2561 4477 4664 1700 Size: 110 597 154 1916 187 2869 Fragments arranged by size: 2869 1916 597 187 154 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5833 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5833 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 2326 2434 794 Size: 1532 108 4193 Fragments arranged by size: 4193 1532 108 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstYI r'GATC_y Cuts at: 1972 2991 3163 3530 3915 3932 5384 5470 5482 Size: 1019 172 367 385 17 1452 86 12 Cuts at: 5482 5828 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1019 385 367 346 172 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2226 513 Size: 552 939 222 4120 Fragments arranged by size: 4120 939 552 222 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2008 3104 487 Size: 683 56 782 1096 3216 Fragments arranged by size: 3216 1096 782 683 56 BtsI GCAGTG_nn' Cuts at: 3349 4143 4163 3349 Size: 794 20 5019 Fragments arranged by size: 5019 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3010 3045 529 Size: 35 2446 35 3317 Fragments arranged by size: 3317 2446 35 35 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DraIII CAC_nnn'GTG Cuts at: 2239 2239 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2126 2563 3280 Size: 54 1425 50 275 18 296 437 717 Cuts at: 3280 4169 5798 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 717 437 296 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2126 2563 3280 1537 Size: 275 314 437 717 4090 Fragments arranged by size: 4090 717 437 314 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2177 3090 3763 1353 Size: 824 913 673 3423 Fragments arranged by size: 3423 913 824 673 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4420 4794 4940 387 Size: 1064 138 2831 374 146 1280 Fragments arranged by size: 2831 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2172 2180 2726 3896 4493 5280 5548 Size: 393 540 8 546 1170 597 787 268 Cuts at: 5548 1239 Size: 1524 Fragments arranged by size: 1524 1170 787 597 546 540 393 268 8 EcoNI CCTnn'n_nnAGG Cuts at: 2336 2336 Size: 5833 EcoO109I rG'GnC_Cy Cuts at: 2167 2341 2759 2776 2167 Size: 174 418 17 5224 Fragments arranged by size: 5224 418 174 17 EcoRI G'AATT_C Cuts at: 3142 3520 3142 Size: 378 5455 EcoRV GAT'ATC Cuts at: 2980 2980 Size: 5833 FspI TGC'GCA Cuts at: 2600 4339 2600 Size: 1739 4094 HaeII r_GCGC'y Cuts at: 1678 4980 1678 Size: 3302 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4474 4506 4548 4580 1373 Size: 32 135 32 2902 32 42 32 2626 Fragments arranged by size: 2902 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2614 3177 3423 669 Size: 988 281 676 563 246 3079 Fragments arranged by size: 3079 988 676 563 281 246 HindIII A'AGCT_T Cuts at: 748 2963 748 Size: 2215 3618 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2611 2886 4947 5131 1305 Size: 1306 275 2061 184 2007 Fragments arranged by size: 2061 2007 1306 275 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4011 Fragments arranged by size: 4011 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 2423 2549 3791 4150 4309 518 Size: 648 975 282 126 1242 359 159 2042 Fragments arranged by size: 2042 1242 975 648 359 282 159 126 MspA1I CmG'CkG Cuts at: 1606 2299 3249 3926 5074 5319 1606 Size: 693 950 677 1148 245 2120 Fragments arranged by size: 2120 1148 950 693 677 245 NaeI GCC'GGC Cuts at: 2409 2563 2409 Size: 154 5679 NcoI C'CATG_G Cuts at: 513 1065 2004 2226 513 Size: 552 939 222 4120 Fragments arranged by size: 4120 939 552 222 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NgoMIV G'CCGG_C Cuts at: 2407 2561 2407 Size: 154 5679 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NspI r_CATG'y Cuts at: 1132 1229 2140 3191 4736 1132 Size: 97 911 1051 1545 2229 Fragments arranged by size: 2229 1545 1051 911 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 2038 2836 1108 Size: 930 798 4105 Fragments arranged by size: 4105 930 798 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2774 2806 3902 3934 5415 5447 2774 Size: 32 1096 32 1481 32 3160 Fragments arranged by size: 3160 1481 1096 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2167 2167 Size: 5833 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5833 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PspOMI G'GGCC_C Cuts at: 2775 2775 Size: 5833 PstI C_TGCA'G Cuts at: 830 2304 3185 830 Size: 1474 881 3478 Fragments arranged by size: 3478 1474 881 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 PvuII CAG'CTG Cuts at: 2299 2299 Size: 5833 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 4081 5793 4081 Size: 1712 4121 SfcI C'TryA_G Cuts at: 826 1045 2300 3181 4316 4997 5188 826 Size: 219 1255 881 1135 681 191 1471 Fragments arranged by size: 1471 1255 1135 881 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3937 4838 5100 820 Size: 197 926 1994 901 262 1553 Fragments arranged by size: 1994 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 3757 5 Size: 47 3705 2081 Fragments arranged by size: 3705 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5833 StyI C'CwwG_G Cuts at: 513 1065 2004 2226 2289 3272 513 Size: 552 939 222 63 983 3074 Fragments arranged by size: 3074 983 939 552 222 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2955 3064 3860 3877 4030 4215 4660 Size: 1255 1375 109 796 17 153 185 445 Cuts at: 4660 325 Size: 1498 Fragments arranged by size: 1498 1375 1255 796 445 185 153 109 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2724 3015 4079 Size: 274 80 33 51 1443 747 291 1064 Cuts at: 4079 5791 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1064 747 291 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5793 5825 5793 Size: 32 5801 Tth111I GACn'n_nGTC Cuts at: 1316 2492 1316 Size: 1176 4657 XbaI T'CTAG_A Cuts at: 2476 3169 2476 Size: 693 5140 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2035 2548 2035 Size: 513 5320 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 3962 3962 Size: 5833 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2572 3641 276 Size: 53 83 186 936 1038 1069 2468 Fragments arranged by size: 2468 1069 1038 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI