(Circular) (Six-base) MAPSORT of: pFN21AE4437.seq Check: 940 from: 1 to: 6064 pFN21AE4437 6064 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3407 1937 Size: 1470 4594 Acc65I G'GTAC_C Cuts at: 3385 3385 Size: 6064 AclI AA'CG_TT Cuts at: 1563 4191 4564 1563 Size: 2628 373 3063 Fragments arranged by size: 3063 2628 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2032 3341 4127 5511 5779 1632 Size: 400 1309 786 1384 268 1917 Fragments arranged by size: 1917 1384 1309 786 400 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5867 AflIII A'CryG_T Cuts at: 1203 3099 4963 5940 1203 Size: 1896 1864 977 1327 Fragments arranged by size: 1896 1864 1327 977 AgeI A'CCGG_T Cuts at: 4895 4895 Size: 6064 AhdI GACnn_n'nnGTC Cuts at: 2983 4793 2983 Size: 1810 4254 AleI CACnn'nnGTG Cuts at: 1166 2576 2972 1166 Size: 1410 396 4258 Fragments arranged by size: 4258 1410 396 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6064 AlwNI CAG_nnn'CTG Cuts at: 2092 2110 2134 2476 3807 5379 2092 Size: 18 24 342 1331 1572 2777 Fragments arranged by size: 2777 1572 1331 342 24 18 ApaLI G'TGCA_C Cuts at: 4121 5277 4121 Size: 1156 4908 ApoI r'AATT_y Cuts at: 1493 2711 3373 3599 3751 5860 1493 Size: 1218 662 226 152 2109 1697 Fragments arranged by size: 2109 1697 1218 662 226 152 AseI AT'TA_AT Cuts at: 160 1032 4618 160 Size: 872 3586 1606 Fragments arranged by size: 3586 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 2053 2841 3337 3389 1870 Size: 73 110 788 496 52 4545 Fragments arranged by size: 4545 788 496 110 73 52 BamHI G'GATC_C Cuts at: 2082 3236 3394 3761 2082 Size: 1154 158 367 4385 Fragments arranged by size: 4385 1154 367 158 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2339 2384 2924 3385 4840 Size: 325 217 627 552 45 540 461 1455 Cuts at: 4840 618 Size: 1842 Fragments arranged by size: 1842 1455 627 552 540 461 325 217 45 BanII G_rGCy'C Cuts at: 1391 2262 2298 3344 3383 1391 Size: 871 36 1046 39 4072 Fragments arranged by size: 4072 1046 871 39 36 BbeI G_GCGC'C Cuts at: 2388 2388 Size: 6064 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2276 928 Size: 971 377 4716 Fragments arranged by size: 4716 971 377 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4255 4289 1903 Size: 34 2318 34 3678 Fragments arranged by size: 3678 2318 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3956 5166 3956 Size: 1210 4854 BclI T'GATC_A Cuts at: 1548 1548 Size: 6064 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4675 136 Size: 107 122 71 631 605 3003 1525 Fragments arranged by size: 3003 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3006 6059 3006 Size: 3053 3011 BlpI GC'TnA_GC Cuts at: 3481 3481 Size: 6064 Bme1580I G_kGCm'C Cuts at: 1790 2869 2927 4125 5281 1790 Size: 1079 58 1198 1156 2573 Fragments arranged by size: 2573 1198 1156 1079 58 BmgBI CAC'GTC Cuts at: 1318 2683 2974 1318 Size: 1365 291 4408 Fragments arranged by size: 4408 1365 291 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2327 2464 2489 Size: 416 53 83 399 24 910 137 25 Cuts at: 2489 2977 3795 4753 442 Size: 488 818 958 1753 Fragments arranged by size: 1753 958 910 818 488 416 399 137 83 53 25 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3286 3286 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2651 3068 3091 4724 1239 Size: 875 537 417 23 1633 2579 Fragments arranged by size: 2579 1633 875 537 417 23 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2399 2519 2780 4189 5054 5352 2399 Size: 120 261 1409 865 298 3111 Fragments arranged by size: 3111 1409 865 298 261 120 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2513 4727 882 Size: 617 1014 2214 2219 Fragments arranged by size: 2219 2214 1014 617 BsaAI yAC'GTr Cuts at: 493 1204 2659 3855 493 Size: 711 1455 1196 2702 Fragments arranged by size: 2702 1455 1196 711 BsaBI GATnn'nnATC Cuts at: 6058 6058 Size: 6064 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2273 2385 3871 4253 Size: 53 83 186 936 740 112 1486 382 Cuts at: 4253 4888 275 Size: 635 1451 Fragments arranged by size: 1486 1451 936 740 635 382 186 112 83 53 BsaWI w'CCGG_w Cuts at: 2079 4497 4895 5169 5316 5727 2079 Size: 2418 398 274 147 411 2416 Fragments arranged by size: 2418 2416 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2183 2204 2213 2234 3089 Size: 30 165 30 780 21 9 21 855 Cuts at: 3089 3119 1178 Size: 30 4123 Fragments arranged by size: 4123 855 780 165 30 30 30 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 2225 3017 3065 1186 Size: 1018 21 792 48 4185 Fragments arranged by size: 4185 1018 792 48 21 BseYI C'CCAG_C Cuts at: 2635 3357 5267 5763 2635 Size: 722 1910 496 2936 Fragments arranged by size: 2936 1910 722 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2144 1163 Size: 573 408 5083 Fragments arranged by size: 5083 573 408 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3514 4275 5303 1540 Size: 275 186 1513 761 1028 2301 Fragments arranged by size: 2301 1513 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2298 4125 4210 5281 2298 Size: 1827 85 1071 3081 Fragments arranged by size: 3081 1827 1071 85 BsmI GAATG_Cn' Cuts at: 3575 3668 3575 Size: 93 5971 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2262 2298 2869 2927 3344 3383 4125 Size: 399 472 36 571 58 417 39 742 Cuts at: 4125 4210 5281 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 571 472 417 399 85 58 39 36 BspEI T'CCGG_A Cuts at: 2079 5727 2079 Size: 3648 2416 BspHI T'CATG_A Cuts at: 2592 2715 3953 5683 2592 Size: 123 1238 1730 2973 Fragments arranged by size: 2973 1730 1238 123 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2166 2741 3419 844 Size: 1322 575 678 3489 Fragments arranged by size: 3489 1322 678 575 BsrBI CCG'CTC Cuts at: 1394 1823 3282 3951 1394 Size: 429 1459 669 3507 Fragments arranged by size: 3507 1459 669 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4559 4733 65 Size: 1183 3311 174 1396 Fragments arranged by size: 3311 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4708 4895 1700 Size: 110 2898 187 2869 Fragments arranged by size: 2898 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2559 96 Size: 2463 3601 BssHII G'CGCG_C Cuts at: 1925 2032 2142 1925 Size: 107 110 5847 Fragments arranged by size: 5847 110 107 BssSI C'ACGA_G Cuts at: 4124 5136 4124 Size: 1012 5052 BstAPI GCAn_nnn'nTGC Cuts at: 794 3202 794 Size: 2408 3656 BstBI TT'CG_AA Cuts at: 4868 4868 Size: 6064 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6064 BstYI r'GATC_y Cuts at: 1972 2082 2485 3006 3236 3394 3761 4146 4163 Size: 110 403 521 230 158 367 385 17 Cuts at: 4163 5615 5701 5713 6059 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 521 403 385 367 346 230 158 110 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2250 2262 2376 2523 3244 513 Size: 552 1185 12 114 147 721 3333 Fragments arranged by size: 3333 1185 721 552 147 114 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2374 2683 487 Size: 683 56 1148 309 3868 Fragments arranged by size: 3868 1148 683 309 56 BtsI GCAGTG_nn' Cuts at: 2412 3580 4374 4394 2412 Size: 1168 794 20 4082 Fragments arranged by size: 4082 1168 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6064 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 3368 3368 Size: 6064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5071 809 Size: 507 3755 1802 Fragments arranged by size: 3755 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2725 3165 Size: 54 1425 50 275 18 318 577 440 Cuts at: 3165 3345 3511 4400 6029 8 Size: 180 166 889 1629 43 Fragments arranged by size: 1629 1425 889 577 440 318 275 180 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3511 1537 Size: 275 1699 4090 Fragments arranged by size: 4090 1699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2639 3248 3994 1353 Size: 1286 609 746 3423 Fragments arranged by size: 3423 1286 746 609 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4651 5025 5171 387 Size: 1064 138 3062 374 146 1280 Fragments arranged by size: 3062 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2296 2296 Size: 6064 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 2114 2651 3068 3091 3341 4127 Size: 393 400 82 537 417 23 250 786 Cuts at: 4127 4724 5511 5779 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 786 597 537 417 400 393 268 250 82 23 EcoNI CCTnn'n_nnAGG Cuts at: 2114 2480 2897 3050 2114 Size: 366 417 153 5128 Fragments arranged by size: 5128 417 366 153 EcoO109I rG'GnC_Cy Cuts at: 2061 2061 Size: 6064 EcoRI G'AATT_C Cuts at: 3373 3751 3373 Size: 378 5686 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3274 3274 Size: 6064 FspI TGC'GCA Cuts at: 3041 4570 3041 Size: 1529 4535 HaeII r_GCGC'y Cuts at: 1678 2102 2110 2388 5211 1678 Size: 424 8 278 2823 2531 Fragments arranged by size: 2823 2531 424 278 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2969 3001 3256 3288 4705 Size: 32 135 32 1397 32 255 32 1417 Cuts at: 4705 4737 4779 4811 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1417 1397 255 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3408 3654 669 Size: 988 281 1470 246 3079 Fragments arranged by size: 3079 1470 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6064 HpaI GTT'AAC Cuts at: 3654 3654 Size: 6064 KasI G'GCGC_C Cuts at: 2384 2384 Size: 6064 KpnI G_GTAC'C Cuts at: 3389 3389 Size: 6064 MfeI C'AATT_G Cuts at: 3663 3663 Size: 6064 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5178 5362 1305 Size: 3873 184 2007 Fragments arranged by size: 3873 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2727 10 Size: 54 1425 343 895 3347 Fragments arranged by size: 3347 1425 895 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2166 2576 2972 4022 4381 4540 518 Size: 648 1000 410 396 1050 359 159 2042 Fragments arranged by size: 2042 1050 1000 648 410 396 359 159 MspA1I CmG'CkG Cuts at: 1606 2071 2092 2252 3228 3480 4157 5305 5550 Size: 465 21 160 976 252 677 1148 245 Cuts at: 5550 1606 Size: 2120 Fragments arranged by size: 2120 1148 976 677 465 252 245 160 21 NarI GG'CG_CC Cuts at: 2385 2385 Size: 6064 NcoI C'CATG_G Cuts at: 513 1065 2376 3244 513 Size: 552 1311 868 3333 Fragments arranged by size: 3333 1311 868 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6064 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 3103 3422 4967 1132 Size: 97 1874 319 1545 2229 Fragments arranged by size: 2229 1874 1545 319 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6064 PciI A'CATG_T Cuts at: 3099 4963 3099 Size: 1864 4200 PflMI CCAn_nnn'nTGG Cuts at: 1108 2092 2377 2476 2785 2911 1108 Size: 984 285 99 309 126 4261 Fragments arranged by size: 4261 984 309 285 126 99 PfoI T'CCnGG_A Cuts at: 2115 2115 Size: 6064 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4165 5646 5678 4133 Size: 32 1481 32 4519 Fragments arranged by size: 4519 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2061 2061 Size: 6064 PshAI GACnn'nnGTC Cuts at: 1613 2621 1613 Size: 1008 5056 PsiI TTA'TAA Cuts at: 3634 3634 Size: 6064 PstI C_TGCA'G Cuts at: 830 2076 2106 2398 3416 830 Size: 1246 30 292 1018 3478 Fragments arranged by size: 3478 1246 1018 292 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 PvuII CAG'CTG Cuts at: 2092 2092 Size: 6064 SacI G_AGCT'C Cuts at: 2298 2298 Size: 6064 SacII CC_GC'GG Cuts at: 2253 2253 Size: 6064 SalI G'TCGA_C Cuts at: 1936 3406 1936 Size: 1470 4594 SapI GCTCTTCn'nnn_ Cuts at: 3248 3248 Size: 6064 SbfI CC_TGCA'GG Cuts at: 3416 3416 Size: 6064 ScaI AGT'ACT Cuts at: 4312 6024 4312 Size: 1712 4352 SfcI C'TryA_G Cuts at: 826 1045 2072 2102 2394 3412 4547 5228 5419 Size: 219 1027 30 292 1018 1135 681 191 Cuts at: 5419 826 Size: 1471 Fragments arranged by size: 1471 1135 1027 1018 681 292 219 191 30 SfoI GGC'GCC Cuts at: 2386 2386 Size: 6064 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6064 SmaI CCC'GGG Cuts at: 1872 2843 3391 1872 Size: 971 548 4545 Fragments arranged by size: 4545 971 548 SmlI C'TyrA_G Cuts at: 820 1017 1943 2414 2534 2795 4168 5069 5331 Size: 197 926 471 120 261 1373 901 262 Cuts at: 5331 820 Size: 1553 Fragments arranged by size: 1553 1373 926 901 471 262 261 197 120 SnaBI TAC'GTA Cuts at: 493 3855 493 Size: 3362 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6064 SphI G_CATG'C Cuts at: 1132 3422 1132 Size: 2290 3774 SspI AAT'ATT Cuts at: 5 52 3988 5 Size: 47 3936 2081 Fragments arranged by size: 3936 2081 47 StuI AGG'CCT Cuts at: 1517 3298 3318 1517 Size: 1781 20 4263 Fragments arranged by size: 4263 1781 20 StyI C'CwwG_G Cuts at: 513 1065 2376 3244 3503 513 Size: 552 1311 868 259 3074 Fragments arranged by size: 3074 1311 868 552 259 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4091 4108 4261 4446 4891 325 Size: 1255 2511 17 153 185 445 1498 Fragments arranged by size: 2511 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2220 2559 4310 Size: 274 80 33 51 1443 243 339 1751 Cuts at: 4310 6022 96 Size: 1712 138 Fragments arranged by size: 1751 1712 1443 339 274 243 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6024 6056 6024 Size: 32 6032 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6064 XbaI T'CTAG_A Cuts at: 3400 3400 Size: 6064 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2374 3157 2374 Size: 783 5281 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6064 XmaI C'CCGG_G Cuts at: 1870 2841 3389 1870 Size: 971 548 4545 Fragments arranged by size: 4545 971 548 XmnI GAAnn'nnTTC Cuts at: 2813 3257 4193 2813 Size: 444 936 4684 Fragments arranged by size: 4684 936 444 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI