Restriction map for
pFN21AE4437
(Linear) (Six-base) MAPSORT of: KIEE4437.seq Check: 8629 from: 1 to: 1359
KIEE4437.orf
With 182 enzymes: SgfI *
July 3, 2012 19:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 1336 1359
Size: 27 1309 23
Fragments arranged by size:
1309 27 23
AflIII A'CryG_T
Cuts at: 0 1094 1359
Size: 1094 265
AhdI GACnn_n'nnGTC
Cuts at: 0 978 1359
Size: 978 381
AleI CACnn'nnGTG
Cuts at: 0 571 967 1359
Size: 571 396 392
Fragments arranged by size:
571 396 392
AlwNI CAG_nnn'CTG
Cuts at: 0 87 105 129 471 1359
Size: 87 18 24 342 888
Fragments arranged by size:
888 342 87 24 18
ApoI r'AATT_y
Cuts at: 0 706 1359
Size: 706 653
AvaI C'yCGr_G
Cuts at: 0 48 836 1332 1359
Size: 48 788 496 27
Fragments arranged by size:
788 496 48 27
BamHI G'GATC_C
Cuts at: 0 77 1231 1359
Size: 77 1154 128
Fragments arranged by size:
1154 128 77
BanI G'GyrC_C
Cuts at: 0 334 379 919 1359
Size: 334 45 540 440
Fragments arranged by size:
540 440 334 45
BanII G_rGCy'C
Cuts at: 0 257 293 1339 1359
Size: 257 36 1046 20
Fragments arranged by size:
1046 257 36 20
BbeI G_GCGC'C
Cuts at: 0 383 1359
Size: 383 976
BbsI GAAGACnn'nnnn_
Cuts at: 0 271 1359
Size: 271 1088
BglII A'GATC_T
Cuts at: 0 1001 1359
Size: 1001 358
Bme1580I G_kGCm'C
Cuts at: 0 864 922 1359
Size: 864 58 437
Fragments arranged by size:
864 437 58
BmgBI CAC'GTC
Cuts at: 0 678 969 1359
Size: 678 291 390
Fragments arranged by size:
678 390 291
BmrI ACTGGGnnnn_n'
Cuts at: 0 322 459 484 972 1359
Size: 322 137 25 488 387
Fragments arranged by size:
488 387 322 137 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1281 1359
Size: 1281 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 646 1063 1086 1359
Size: 109 537 417 23 273
Fragments arranged by size:
537 417 273 109 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 514 775 1359
Size: 394 120 261 584
Fragments arranged by size:
584 394 261 120
BsaI GGTCTCn'nnnn_
Cuts at: 0 508 1359
Size: 508 851
BsaAI yAC'GTr
Cuts at: 0 654 1359
Size: 654 705
BsaHI Gr'CG_yC
Cuts at: 0 268 380 1359
Size: 268 112 979
Fragments arranged by size:
979 268 112
BsaWI w'CCGG_w
Cuts at: 0 74 1359
Size: 74 1285
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 178 199 208 229 1084 1114 1359
Size: 178 21 9 21 855 30 245
Fragments arranged by size:
855 245 178 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 199 220 1012 1060 1359
Size: 199 21 792 48 299
Fragments arranged by size:
792 299 199 48 21
BseYI C'CCAG_C
Cuts at: 0 630 1352 1359
Size: 630 722 7
Fragments arranged by size:
722 630 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1359
Size: 139 1220
BsiHKAI G_wGCw'C
Cuts at: 0 293 1359
Size: 293 1066
Bsp1286I G_dGCh'C
Cuts at: 0 257 293 864 922 1339 1359
Size: 257 36 571 58 417 20
Fragments arranged by size:
571 417 257 58 36 20
BspEI T'CCGG_A
Cuts at: 0 74 1359
Size: 74 1285
BspHI T'CATG_A
Cuts at: 0 587 710 1359
Size: 587 123 649
Fragments arranged by size:
649 587 123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 161 736 1359
Size: 161 575 623
Fragments arranged by size:
623 575 161
BsrBI CCG'CTC
Cuts at: 0 1277 1359
Size: 1277 82
BsrGI T'GTAC_A
Cuts at: 0 554 1359
Size: 554 805
BssHII G'CGCG_C
Cuts at: 0 27 137 1359
Size: 27 110 1222
Fragments arranged by size:
1222 110 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1197 1359
Size: 1197 162
BstYI r'GATC_y
Cuts at: 0 77 480 1001 1231 1359
Size: 77 403 521 230 128
Fragments arranged by size:
521 403 230 128 77
BtgI C'CryG_G
Cuts at: 0 245 257 371 518 1239 1359
Size: 245 12 114 147 721 120
Fragments arranged by size:
721 245 147 120 114 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 369 678 1359
Size: 369 309 681
Fragments arranged by size:
681 369 309
BtsI GCAGTG_nn'
Cuts at: 0 407 1359
Size: 407 952
EaeI y'GGCC_r
Cuts at: 0 143 720 1160 1340 1359
Size: 143 577 440 180 19
Fragments arranged by size:
577 440 180 143 19
EarI CTCTTCn'nnn_
Cuts at: 0 634 1243 1359
Size: 634 609 116
Fragments arranged by size:
634 609 116
EcoICRI GAG'CTC
Cuts at: 0 291 1359
Size: 291 1068
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 109 646 1063 1086 1336 1359
Size: 27 82 537 417 23 250 23
Fragments arranged by size:
537 417 250 82 27 23 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 109 475 892 1045 1359
Size: 109 366 417 153 314
Fragments arranged by size:
417 366 314 153 109
EcoO109I rG'GnC_Cy
Cuts at: 0 56 1359
Size: 56 1303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1269 1359
Size: 1269 90
FspI TGC'GCA
Cuts at: 0 1036 1359
Size: 1036 323
HaeII r_GCGC'y
Cuts at: 0 97 105 383 1359
Size: 97 8 278 976
Fragments arranged by size:
976 278 97 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 964 996 1251 1283 1359
Size: 964 32 255 32 76
Fragments arranged by size:
964 255 76 32 32
KasI G'GCGC_C
Cuts at: 0 379 1359
Size: 379 980
MscI TGG'CCA
Cuts at: 0 722 1359
Size: 722 637
MslI CAynn'nnrTG
Cuts at: 0 161 571 967 1359
Size: 161 410 396 392
Fragments arranged by size:
410 396 392 161
MspA1I CmG'CkG
Cuts at: 0 66 87 247 1223 1359
Size: 66 21 160 976 136
Fragments arranged by size:
976 160 136 66 21
NarI GG'CG_CC
Cuts at: 0 380 1359
Size: 380 979
NcoI C'CATG_G
Cuts at: 0 371 1239 1359
Size: 371 868 120
Fragments arranged by size:
868 371 120
NspI r_CATG'y
Cuts at: 0 1098 1359
Size: 1098 261
PciI A'CATG_T
Cuts at: 0 1094 1359
Size: 1094 265
PflMI CCAn_nnn'nTGG
Cuts at: 0 87 372 471 780 906 1359
Size: 87 285 99 309 126 453
Fragments arranged by size:
453 309 285 126 99 87
PfoI T'CCnGG_A
Cuts at: 0 110 1359
Size: 110 1249
PpuMI rG'GwC_Cy
Cuts at: 0 56 1359
Size: 56 1303
PshAI GACnn'nnGTC
Cuts at: 0 616 1359
Size: 616 743
PstI C_TGCA'G
Cuts at: 0 71 101 393 1359
Size: 71 30 292 966
Fragments arranged by size:
966 292 71 30
PvuII CAG'CTG
Cuts at: 0 87 1359
Size: 87 1272
SacI G_AGCT'C
Cuts at: 0 293 1359
Size: 293 1066
SacII CC_GC'GG
Cuts at: 0 248 1359
Size: 248 1111
SapI GCTCTTCn'nnn_
Cuts at: 0 1243 1359
Size: 1243 116
SfcI C'TryA_G
Cuts at: 0 67 97 389 1359
Size: 67 30 292 970
Fragments arranged by size:
970 292 67 30
SfoI GGC'GCC
Cuts at: 0 381 1359
Size: 381 978
SmaI CCC'GGG
Cuts at: 0 838 1359
Size: 838 521
SmlI C'TyrA_G
Cuts at: 0 409 529 790 1359
Size: 409 120 261 569
Fragments arranged by size:
569 409 261 120
StuI AGG'CCT
Cuts at: 0 1293 1313 1359
Size: 1293 20 46
Fragments arranged by size:
1293 46 20
StyI C'CwwG_G
Cuts at: 0 371 1239 1359
Size: 371 868 120
Fragments arranged by size:
868 371 120
TatI w'GTAC_w
Cuts at: 0 215 554 1359
Size: 215 339 805
Fragments arranged by size:
805 339 215
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 369 1152 1359
Size: 369 783 207
Fragments arranged by size:
783 369 207
XmaI C'CCGG_G
Cuts at: 0 836 1359
Size: 836 523
XmnI GAAnn'nnTTC
Cuts at: 0 808 1252 1359
Size: 808 444 107
Fragments arranged by size:
808 444 107
Enzymes that do cut:
AcuI AflIII AhdI AleI AlwNI ApoI AvaI BamHI
BanI BanII BbeI BbsI BglII Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrGI BssHII BstAPI BstYI BtgI BtgZI BtsI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I KasI MscI MslI MspA1I NarI NcoI
NspI PciI PflMI PfoI PpuMI PshAI PstI PvuII
SacI SacII SapI SfcI SfoI SmaI SmlI StuI
StyI TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
BmtI Bpu10I BsaBI BsiEI BsiWI BsmI BsmBI BsrDI
BsrFI BssSI BstBI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XhoI ZraI