################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvrII BalI BamHI BbrPI BbsI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI HphI KpnI MamI MfeI MflI MluNI MroI MscI MseI MslI MunI NcoI NdeI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AfaI 1048 GTAC GT^AC AflIII 774 ACGCGT A^C[GA][CT]GT AhdI 538 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 685 GGATC GGATC(4/5) Aor51HI 755 AGCGCT AGC^GCT ApaI 419 GGGCCC GGGCC^C AspEI 538 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1078 GAGCTC G[AT]GC[AT]^C BbvCI 948 CCTCAGC CCTCAGC(-5/-2) BfaI 193 CTAG C^TAG BseRI 850 CTCCTC GAGGAG(10/8) BsiHKAI 1078 GAGCTC G[AT]GC[AT]^C BsmBI 310 GAGACG CGTCTC(1/5) BsrBI 842 GAGCGG CCGCTC(-3/-3) DpnII 686 GATC ^GATC DraIII 657 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 538 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1009 GAAGAG CTCTTC(1/4) Eco47III 755 AGCGCT AGC^GCT EcoNI 291 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 95 CCTTGG C^C[AT][AT]GG HinfI 865 GATTC G^A[ACGT]TC Ksp632I 1009 GAAGAG CTCTTC(1/4) MaeI 193 CTAG C^TAG MaeIII 358 GTCAC ^GT[ACGT]AC MboI 686 GATC ^GATC MboII 1009 GAAGA GAAGA(8/7) MluI 774 ACGCGT A^CGCGT NaeI 340 GCCGGC GCC^GGC NdeII 686 GATC ^GATC NgoMIV 340 GCCGGC G^CCGGC NheI 192 GCTAGC G^CTAGC NotI 974 GCGGCCGC GC^GGCCGC NruI 729 TCGCGA TCG^CGA RsaI 1048 GTAC GT^AC SacI 1078 GAGCTC GAGCT^C Sau3AI 686 GATC ^GATC SmlI 826 CTCAAG C^T[CT][GA]AG StyI 95 CCTTGG C^C[AT][AT]GG TfiI 865 GATTC G^A[AT]TC Tsp45I 358 GTCAC ^GT[GC]AC Tsp509I 256 AATT ^AATT ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AviII 698 TGCGCA TGC^GCA 1070 TGCGCA BlpI 169 GCTGAGC GC^T[ACGT]AGC 1080 GCTCAGC BpmI 261 CTCCAG CTGGAG(16/14) 751 CTGGAG Bpu1102I 169 GCTGAGC GC^T[ACGT]AGC 1080 GCTCAGC BsgI 610 CTGCAC GTGCAG(16/14) 1000 GTGCAG BsmAI 310 GAGAC GTCTC(1/5) 587 GAGAC BsmFI 536 GGGAC GGGAC(10/14) 579 GGGAC BspMI 703 ACCTGC ACCTGC(4/8) 962 ACCTGC BsrFI 340 GCCGGC [GA]^CCGG[CT] 898 ACCGGC BssHII 692 GCGCGC G^CGCGC 740 GCGCGC BstAPI 656 GCACCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 711 GCAGAAGCTGC BstF5I 288 CATCC GGATG(2/0) 591 CATCC CelII 169 GCTGAGC GC^T[ACGT]AGC 1080 GCTCAGC Cfr10I 340 GCCGGC [GA]^CCGG[CT] 898 ACCGGC DraII 419 GGGCCCC [GA]G^G[ACGT]CC[CT] 800 AGGCCCT EagI 338 CGGCCG C^GGCCG 975 CGGCCG EclXI 338 CGGCCG C^GGCCG 975 CGGCCG Eco52I 338 CGGCCG C^GGCCG 975 CGGCCG EcoO109I 419 GGGCCCC [GA]G^G[ACGT]CC[CT] 800 AGGCCCT FokI 288 CATCC GGATG(9/13) 591 CATCC FspI 698 TGCGCA TGC^GCA 1070 TGCGCA MaeII 62 ACGT A^CGT 524 ACGT NlaIII 689 CATG CATG^ 780 CATG PstI 120 CTGCAG CTGCA^G 388 CTGCAG SfaNI 116 GCATC GCATC(5/9) 287 GCATC SfcI 120 CTGCAG C^T[GA][CT]AG 388 CTGCAG StuI 275 AGGCCT AGG^CCT 365 AGGCCT TaqI 547 TCGA T^CGA 1007 TCGA TthHB8I 547 TCGA T^CGA 1007 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BsiEI BsiYI BslI BsoBI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DsaI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI KspI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIV SacII Sau96I ScrFI SfoI TseI TspRI