(Circular) (Six-base) MAPSORT of: pFN21AE1502.seq Check: 2529 from: 1 to: 5800 pFN21AE1502 5800 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2717 2717 Size: 5800 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3143 1937 Size: 1206 4594 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3863 5247 5515 1632 Size: 2231 1384 268 1917 Fragments arranged by size: 2231 1917 1384 268 AfeI AGC'GCT Cuts at: 2762 2762 Size: 5800 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 2779 4699 5676 1203 Size: 1576 1920 977 1327 Fragments arranged by size: 1920 1576 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 2548 4529 2548 Size: 1981 3819 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2825 1184 Size: 1641 4159 AlwNI CAG_nnn'CTG Cuts at: 2124 2722 2961 3093 3543 5115 2124 Size: 598 239 132 450 1572 2809 Fragments arranged by size: 2809 1572 598 450 239 132 ApaI G_GGCC'C Cuts at: 2428 2428 Size: 5800 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 3109 3335 3487 5596 1493 Size: 1616 226 152 2109 1697 Fragments arranged by size: 2109 1697 1616 226 152 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2202 2216 3125 1870 Size: 73 87 172 14 909 4545 Fragments arranged by size: 4545 909 172 87 73 14 BamHI G'GATC_C Cuts at: 3130 3497 3130 Size: 367 5433 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2207 2348 2410 2453 2771 Size: 325 217 627 420 141 62 43 318 Cuts at: 2771 2789 2988 3121 4576 618 Size: 18 199 133 1455 1842 Fragments arranged by size: 1842 1455 627 420 325 318 217 199 141 133 62 43 18 BanII G_rGCy'C Cuts at: 1391 2428 2483 3087 3119 1391 Size: 1037 55 604 32 4072 Fragments arranged by size: 4072 1037 604 55 32 BbeI G_GCGC'C Cuts at: 2211 2352 2414 2775 2211 Size: 141 62 361 5236 Fragments arranged by size: 5236 361 141 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4829 BbvCI CC'TCA_GC Cuts at: 2954 2954 Size: 5800 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3991 4025 1903 Size: 34 50 34 1970 34 3678 Fragments arranged by size: 3678 1970 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 1548 Size: 5800 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2419 2451 2478 Size: 107 122 71 631 605 747 32 27 Cuts at: 2478 4411 136 Size: 1933 1525 Fragments arranged by size: 1933 1525 747 631 605 122 107 71 32 27 BglII A'GATC_T Cuts at: 5795 5795 Size: 5800 BlpI GC'TnA_GC Cuts at: 2175 3086 3217 2175 Size: 911 131 4758 Fragments arranged by size: 4758 911 131 Bme1580I G_kGCm'C Cuts at: 1790 2060 2428 2792 2991 3861 5017 1790 Size: 270 368 364 199 870 1156 2573 Fragments arranged by size: 2573 1156 870 368 364 270 199 BmgBI CAC'GTC Cuts at: 1318 2068 2530 1318 Size: 750 462 4588 Fragments arranged by size: 4588 750 462 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3531 4489 442 Size: 416 53 83 399 24 2114 958 1753 Fragments arranged by size: 2114 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2201 1056 Size: 1145 4655 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2870 2870 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2251 2777 4460 1239 Size: 1012 526 1683 2579 Fragments arranged by size: 2579 1683 1012 526 Bpu10I CC'TnA_GC Cuts at: 2954 2954 Size: 5800 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2816 3925 4790 5088 2816 Size: 1109 865 298 3528 Fragments arranged by size: 3528 1109 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4463 882 Size: 617 2964 2219 Fragments arranged by size: 2964 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3591 493 Size: 711 2387 2702 Fragments arranged by size: 2702 2387 711 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2208 2349 2411 2772 Size: 53 83 186 936 675 141 62 361 Cuts at: 2772 3607 3989 4624 275 Size: 835 382 635 1451 Fragments arranged by size: 1451 936 835 675 635 382 361 186 141 83 62 53 BsaWI w'CCGG_w Cuts at: 4233 4631 4905 5052 5463 4233 Size: 398 274 147 411 4570 Fragments arranged by size: 4570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5575 Fragments arranged by size: 5575 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2846 1186 Size: 1660 4140 BseYI C'CCAG_C Cuts at: 5003 5499 5003 Size: 496 5304 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2600 3026 1163 Size: 573 864 426 3937 Fragments arranged by size: 3937 864 573 426 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2346 2926 2983 3250 4011 5039 Size: 275 186 345 580 57 267 761 1028 Cuts at: 5039 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 580 345 275 267 186 57 BsiHKAI G_wGCw'C Cuts at: 3087 3861 3946 5017 3087 Size: 774 85 1071 3870 Fragments arranged by size: 3870 1071 774 85 BsmI GAATG_Cn' Cuts at: 3311 3404 3311 Size: 93 5707 BsmBI CGTCTCn'nnnn_ Cuts at: 2309 2309 Size: 5800 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2428 2483 2792 2991 3087 3119 Size: 399 270 368 55 309 199 96 32 Cuts at: 3119 3861 3946 5017 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 368 309 270 199 96 85 55 32 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2717 2976 3155 844 Size: 1873 259 179 3489 Fragments arranged by size: 3489 1873 259 179 BsrBI CCG'CTC Cuts at: 1394 1823 2849 3687 1394 Size: 429 1026 838 3507 Fragments arranged by size: 3507 1026 838 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4295 4469 65 Size: 1183 3047 174 1396 Fragments arranged by size: 3047 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2345 2903 4444 4631 1700 Size: 110 535 558 1541 187 2869 Fragments arranged by size: 2869 1541 558 535 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 2697 2745 1925 Size: 772 48 4980 Fragments arranged by size: 4980 772 48 BssSI C'ACGA_G Cuts at: 3860 4872 3860 Size: 1012 4788 BstAPI GCAn_nnn'nTGC Cuts at: 794 2667 2722 794 Size: 1873 55 3872 Fragments arranged by size: 3872 1873 55 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstYI r'GATC_y Cuts at: 1972 3130 3497 3882 3899 5351 5437 5449 5795 Size: 1158 367 385 17 1452 86 12 346 Cuts at: 5795 1972 Size: 1977 Fragments arranged by size: 1977 1452 1158 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2416 2475 2677 2767 2983 513 Size: 552 1351 59 202 90 216 3330 Fragments arranged by size: 3330 1351 552 216 202 90 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5061 Fragments arranged by size: 5061 683 56 BtsI GCAGTG_nn' Cuts at: 2337 2402 3316 4110 4130 2337 Size: 65 914 794 20 4007 Fragments arranged by size: 4007 914 794 65 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DraIII CAC_nnn'GTG Cuts at: 2667 2667 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4807 809 Size: 507 3491 1802 Fragments arranged by size: 3491 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2343 2640 Size: 54 1425 50 275 18 315 198 297 Cuts at: 2640 2980 3247 4136 5765 8 Size: 340 267 889 1629 43 Fragments arranged by size: 1629 1425 889 340 315 297 275 267 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2343 2980 3247 1537 Size: 275 531 637 267 4090 Fragments arranged by size: 4090 637 531 275 267 EarI CTCTTCn'nnn_ Cuts at: 1353 3009 3730 1353 Size: 1656 721 3423 Fragments arranged by size: 3423 1656 721 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2088 2240 4387 4761 4907 387 Size: 1064 138 499 152 2147 374 146 1280 Fragments arranged by size: 2147 1280 1064 499 374 152 146 138 EcoICRI GAG'CTC Cuts at: 3085 3085 Size: 5800 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2251 2777 3863 4460 5247 5515 1239 Size: 393 619 526 1086 597 787 268 1524 Fragments arranged by size: 1524 1086 787 619 597 526 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2300 2300 Size: 5800 EcoO109I rG'GnC_Cy Cuts at: 2425 2806 2425 Size: 381 5419 EcoRI G'AATT_C Cuts at: 3109 3487 3109 Size: 378 5422 FspI TGC'GCA Cuts at: 2705 3077 4306 2705 Size: 372 1229 4199 Fragments arranged by size: 4199 1229 372 HaeII r_GCGC'y Cuts at: 1678 2211 2352 2414 2499 2764 2775 2946 4947 Size: 533 141 62 85 265 11 171 2001 Cuts at: 4947 1678 Size: 2531 Fragments arranged by size: 2531 2001 533 265 171 141 85 62 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2534 2566 4441 4473 4515 Size: 32 135 32 962 32 1875 32 42 Cuts at: 4515 4547 1373 Size: 32 2626 Fragments arranged by size: 2626 1875 962 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3144 3390 669 Size: 988 281 1206 246 3079 Fragments arranged by size: 3079 1206 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KasI G'GCGC_C Cuts at: 2207 2348 2410 2771 2207 Size: 141 62 361 5236 Fragments arranged by size: 5236 361 141 62 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 2779 5676 2779 Size: 2897 2903 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4914 5098 1305 Size: 3609 184 2007 Fragments arranged by size: 3609 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3978 Fragments arranged by size: 3978 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3758 4117 4276 518 Size: 648 2592 359 159 2042 Fragments arranged by size: 2592 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2256 2418 2477 2958 2985 3066 3216 3893 Size: 650 162 59 481 27 81 150 677 Cuts at: 3893 5041 5286 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 650 481 245 162 150 81 59 27 NaeI GCC'GGC Cuts at: 2347 2347 Size: 5800 NarI GG'CG_CC Cuts at: 2208 2349 2411 2772 2208 Size: 141 62 361 5236 Fragments arranged by size: 5236 361 141 62 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5248 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NgoMIV G'CCGG_C Cuts at: 2345 2345 Size: 5800 NheI G'CTAG_C Cuts at: 1052 2197 1052 Size: 1145 4655 NotI GC'GGCC_GC Cuts at: 2980 3247 2980 Size: 267 5533 NruI TCG'CGA Cuts at: 2736 2736 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 3158 4703 1132 Size: 97 1929 1545 2229 Fragments arranged by size: 2229 1929 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5800 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5800 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2219 2251 3869 3901 5382 5414 2219 Size: 32 1618 32 1481 32 2605 Fragments arranged by size: 2605 1618 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PspOMI G'GGCC_C Cuts at: 2424 2424 Size: 5800 PstI C_TGCA'G Cuts at: 830 2129 2397 3152 830 Size: 1299 268 755 3478 Fragments arranged by size: 3478 1299 755 268 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 SacI G_AGCT'C Cuts at: 3087 3087 Size: 5800 SacII CC_GC'GG Cuts at: 2419 2478 2986 2419 Size: 59 508 5233 Fragments arranged by size: 5233 508 59 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 4048 5760 4048 Size: 1712 4088 SfcI C'TryA_G Cuts at: 826 1045 2125 2393 3148 4283 4964 5155 826 Size: 219 1080 268 755 1135 681 191 1471 Fragments arranged by size: 1471 1135 1080 755 681 268 219 191 SfoI GGC'GCC Cuts at: 2209 2350 2412 2773 2209 Size: 141 62 361 5236 Fragments arranged by size: 5236 361 141 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 3127 1872 Size: 1255 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2831 3904 4805 5067 820 Size: 197 926 888 1073 901 262 1553 Fragments arranged by size: 1553 1073 926 901 888 262 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 2282 2372 1517 Size: 765 90 4945 Fragments arranged by size: 4945 765 90 StyI C'CwwG_G Cuts at: 513 1065 2100 3239 513 Size: 552 1035 1139 3074 Fragments arranged by size: 3074 1139 1035 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3827 3844 3997 4182 4627 325 Size: 1255 2247 17 153 185 445 1498 Fragments arranged by size: 2247 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3052 4046 5758 Size: 274 80 33 51 1443 1075 994 1712 Cuts at: 5758 96 Size: 138 Fragments arranged by size: 1712 1443 1075 994 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5760 5792 5760 Size: 32 5768 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5800 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 3125 1870 Size: 1255 4545 XmnI GAAnn'nnTTC Cuts at: 3929 3929 Size: 5800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI