################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsiEI BsiWI BsmBI BsmI BspDI BspHI BsrDI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MluNI MscI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SapI ScaI SfuI SgrAI SnaBI SpeI SspI SwaI TaqI TfiI Tru9I Tsp509I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 307 GTCTAC GT^[AC][GT]AC AccIII 738 TCCGGA T^CCGGA AfaI 807 GTAC GT^AC AflIII 512 ACATGT A^C[GA][CT]GT Alw44I 964 GTGCAC G^TGCAC ApaLI 964 GTGCAC G^TGCAC AspI 703 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 493 CACGTG CAC^GTG BcgI 948 GCAGGAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 493 CACGTG [CT]AC^GT[GA] BsaWI 738 TCCGGA [AT]^CCGG[AT] BseAI 738 TCCGGA T^CCGGA BseRI 646 GAGGAG GAGGAG(10/8) BsmAI 831 GTCTC GTCTC(1/5) Bsp1407I 806 TGTACA T^GTACA BspEI 738 TCCGGA T^CCGGA BspLU11I 512 ACATGT A^CATGT BspMI 441 GCAGGT ACCTGC(4/8) BsrGI 806 TGTACA T^GTACA BstAPI 224 GCATGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 719 GGATCT [GA]^GATC[CT] DsaI 194 CCGCGG C^C[GA][CT]GG EarI 931 CTCTTC CTCTTC(1/4) EcoT14I 636 CCTTGG C^C[AT][AT]GG HinfI 681 GACTC G^A[ACGT]TC HphI 633 TCACC GGTGA(8/7) Ksp632I 931 CTCTTC CTCTTC(1/4) KspI 194 CCGCGG CCGC^GG MaeII 494 ACGT A^CGT MboII 932 TCTTC GAAGA(8/7) MflI 719 GGATCT [GA]^GATC[CT] MroI 738 TCCGGA T^CCGGA MslI 507 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflFI 703 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 705 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 681 GACTC GAGTC(4/5) PmaCI 493 CACGTG CAC^GTG PmlI 493 CACGTG CAC^GTG PstI 370 CTGCAG CTGCA^G RsaI 807 GTAC GT^AC SacI 238 GAGCTC GAGCT^C SacII 194 CCGCGG CCGC^GG SbfI 369 CCTGCAGG CCTGCA^GG SexAI 704 ACCAGGT A^CC[AT]GGT SfcI 370 CTGCAG C^T[GA][CT]AG SfiI 201 GGCCGCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 351 CCCGGG CCC^GGG SmlI 550 CTCAAG C^T[CT][GA]AG SphI 224 GCATGC GCATG^C Sse8387I 369 CCTGCAGG CCTGCA^GG SspBI 806 TGTACA T^GTACA StuI 477 AGGCCT AGG^CCT StyI 636 CCTTGG C^C[AT][AT]GG Tth111I 703 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 705 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 506 CCATGAACATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 719 GGATCT [GA]^GATC[CT] XmaCI 351 CCCGGG C^CCGGG XmaI 351 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 888 GATCC GGATC(4/5) 719 GGATC ApaI 169 GGGCCC GGGCC^C 750 GGGCCC BanI 217 GGCGCC G^G[CT][GA]CC 427 GGCGCC BbeI 217 GGCGCC GGCGC^C 427 GGCGCC BglI 193 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 202 GCCGCAGTGGC BsgI 141 CTGCAC GTGCAG(16/14) 775 CTGCAC BsmFI 413 GGGAC GGGAC(10/14) 914 GGGAC BsrBI 715 GAGCGG CCGCTC(-3/-3) 745 GAGCGG BsrFI 177 GCCGGT [GA]^CCGG[CT] 605 ACCGGC BsrI 81 ACTGG ACTGG(1/-1) 699 ACTGG BssHII 109 GCGCGC G^CGCGC 111 GCGCGC Cfr10I 177 GCCGGT [GA]^CCGG[CT] 605 ACCGGC DdeI 468 CTCAG C^T[ACGT]AG 596 CTGAG DpnII 720 GATC ^GATC 888 GATC HgaI 415 GACGC GACGC(5/10) 305 GCGTC KasI 217 GGCGCC G^GCGCC 427 GGCGCC MaeIII 592 GTCAC ^GT[ACGT]AC 791 GTGAC MboI 720 GATC ^GATC 888 GATC NarI 217 GGCGCC GG^CGCC 427 GGCGCC NdeII 720 GATC ^GATC 888 GATC NspI 224 GCATGC [GA]CATG^[CT] 512 ACATGT PpuMI 707 AGGTCCT [GA]G^G[AT]CC[CT] 914 GGGACCC Sau3AI 720 GATC ^GATC 888 GATC SfaNI 978 GATGC GCATC(5/9) 630 GCATC SfoI 217 GGCGCC GGC^GCC 427 GGCGCC Tsp45I 592 GTCAC ^GT[GC]AC 791 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AspHI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI TspRI