(Circular) (Six-base) MAPSORT of: pFN21AE4326.seq Check: 3919 from: 1 to: 5689 pFN21AE4326 5689 bp With 182 enzymes: SgfI * March 28, 2012 14:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2313 3032 1937 Size: 376 719 4594 Fragments arranged by size: 4594 719 376 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3752 5136 5404 1632 Size: 2120 1384 268 1917 Fragments arranged by size: 2120 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 2517 4588 5565 1203 Size: 1314 2071 977 1327 Fragments arranged by size: 2071 1327 1314 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2114 2146 2114 Size: 32 5657 AlwNI CAG_nnn'CTG Cuts at: 2338 2383 2704 2716 3432 5004 2338 Size: 45 321 12 716 1572 3023 Fragments arranged by size: 3023 1572 716 321 45 12 ApaI G_GGCC'C Cuts at: 2178 2759 2178 Size: 581 5108 ApaLI G'TGCA_C Cuts at: 2969 3746 4902 2969 Size: 777 1156 3756 Fragments arranged by size: 3756 1156 777 ApoI r'AATT_y Cuts at: 1493 2998 3224 3376 5485 1493 Size: 1505 226 152 2109 1697 Fragments arranged by size: 2109 1697 1505 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2153 2356 2924 3014 1870 Size: 73 210 203 568 90 4545 Fragments arranged by size: 4545 568 210 203 90 73 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2222 2432 3010 4465 618 Size: 325 217 627 435 210 578 1455 1842 Fragments arranged by size: 1842 1455 627 578 435 325 217 210 BanII G_rGCy'C Cuts at: 1391 2178 2247 2363 2658 2759 2905 3008 1391 Size: 787 69 116 295 101 146 103 4072 Fragments arranged by size: 4072 787 295 146 116 103 101 69 BbeI G_GCGC'C Cuts at: 2226 2436 2226 Size: 210 5479 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4718 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2942 2976 3880 3914 1903 Size: 34 50 34 921 34 904 34 3678 Fragments arranged by size: 3678 921 904 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2204 2213 4300 Size: 107 122 71 631 605 532 9 2087 Cuts at: 4300 136 Size: 1525 Fragments arranged by size: 2087 1525 631 605 532 122 107 71 9 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 2178 2402 2759 2973 3750 4906 1790 Size: 388 224 357 214 777 1156 2573 Fragments arranged by size: 2573 1156 777 388 357 224 214 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2096 3420 4378 Size: 416 53 83 399 24 679 1324 958 Cuts at: 4378 442 Size: 1753 Fragments arranged by size: 1753 1324 958 679 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2743 2743 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2312 2669 2738 2783 4349 1239 Size: 1073 357 69 45 1566 2579 Fragments arranged by size: 2579 1566 1073 357 69 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2540 3814 4679 4977 2540 Size: 1274 865 298 3252 Fragments arranged by size: 3252 1274 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4352 882 Size: 617 2853 2219 Fragments arranged by size: 2853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2500 3480 493 Size: 711 1296 980 2702 Fragments arranged by size: 2702 1296 980 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2223 2310 2421 2433 Size: 53 83 186 936 690 87 111 12 Cuts at: 2433 3496 3878 4513 275 Size: 1063 382 635 1451 Fragments arranged by size: 1451 1063 936 690 635 382 186 111 87 83 53 12 BsaWI w'CCGG_w Cuts at: 2743 4122 4520 4794 4941 5352 2743 Size: 1379 398 274 147 411 3080 Fragments arranged by size: 3080 1379 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 2345 2375 2472 Size: 30 165 30 711 30 201 30 97 Cuts at: 2472 2502 2759 2789 2849 2879 1178 Size: 30 257 30 60 30 3988 Fragments arranged by size: 3988 711 257 201 165 97 60 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2666 1186 Size: 1480 4209 BseYI C'CCAG_C Cuts at: 2976 4892 5388 2976 Size: 1916 496 3277 Fragments arranged by size: 3277 1916 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2131 2765 1163 Size: 573 395 634 4087 Fragments arranged by size: 4087 634 573 395 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2247 2769 2973 3750 3835 4906 2247 Size: 522 204 777 85 1071 3030 Fragments arranged by size: 3030 1071 777 522 204 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2178 2247 2363 2402 2658 2759 2769 Size: 399 388 69 116 39 256 101 10 Cuts at: 2769 2905 2973 3008 3750 3835 4906 1391 Size: 136 68 35 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 388 256 136 116 101 85 69 68 39 35 10 BspEI T'CCGG_A Cuts at: 2743 5352 2743 Size: 2609 3080 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2437 2977 3044 844 Size: 1593 540 67 3489 Fragments arranged by size: 3489 1593 540 67 BsrBI CCG'CTC Cuts at: 1394 1823 2722 2752 3576 1394 Size: 429 899 30 824 3507 Fragments arranged by size: 3507 899 824 429 30 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2182 2610 4333 4520 1700 Size: 110 372 428 1723 187 2869 Fragments arranged by size: 2869 1723 428 372 187 110 BsrGI T'GTAC_A Cuts at: 96 2811 96 Size: 2715 2974 BssHII G'CGCG_C Cuts at: 1925 2114 2116 1925 Size: 189 2 5498 Fragments arranged by size: 5498 189 2 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 2235 794 Size: 1441 4248 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstYI r'GATC_y Cuts at: 1972 2724 3019 3386 3771 3788 5240 5326 5338 Size: 752 295 367 385 17 1452 86 12 Cuts at: 5338 5684 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 752 385 367 346 295 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2199 513 Size: 552 1134 4003 Fragments arranged by size: 4003 1134 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2478 487 Size: 683 56 1252 3698 Fragments arranged by size: 3698 1252 683 56 BtsI GCAGTG_nn' Cuts at: 2217 2542 3205 3999 4019 2217 Size: 325 663 794 20 3887 Fragments arranged by size: 3887 794 663 325 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2163 2205 2214 Size: 54 1425 50 275 18 333 42 9 Cuts at: 2214 2329 2542 2577 3136 4025 5654 8 Size: 115 213 35 559 889 1629 43 Fragments arranged by size: 1629 1425 889 559 333 275 213 115 54 50 43 42 35 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2942 3619 1353 Size: 1589 677 3423 Fragments arranged by size: 3423 1589 677 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2245 2245 Size: 5689 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 2669 2738 2783 3752 4349 5136 Size: 393 680 357 69 45 969 597 787 Cuts at: 5136 5404 1239 Size: 268 1524 Fragments arranged by size: 1524 969 787 680 597 393 357 268 69 45 EcoO109I rG'GnC_Cy Cuts at: 2175 2713 2755 2756 2920 2175 Size: 538 42 1 164 4944 Fragments arranged by size: 4944 538 164 42 1 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2161 2226 2384 2416 2436 4836 1678 Size: 483 65 158 32 20 2400 2531 Fragments arranged by size: 2531 2400 483 158 65 32 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2456 2488 4330 4362 4404 Size: 32 135 32 884 32 1842 32 42 Cuts at: 4404 4436 1373 Size: 32 2626 Fragments arranged by size: 2626 1842 884 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KasI G'GCGC_C Cuts at: 2222 2432 2222 Size: 210 5479 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4803 4987 1305 Size: 3498 184 2007 Fragments arranged by size: 3498 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3867 Fragments arranged by size: 3867 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2516 3647 4006 4165 518 Size: 648 1350 1131 359 159 2042 Fragments arranged by size: 2042 1350 1131 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2053 2062 2071 2136 2201 2980 3105 Size: 432 15 9 9 65 65 779 125 Cuts at: 3105 3782 4930 5175 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 779 677 432 245 125 65 65 15 9 9 NarI GG'CG_CC Cuts at: 2223 2433 2223 Size: 210 5479 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5137 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 2233 2521 3047 4592 1132 Size: 97 1004 288 526 1545 2229 Fragments arranged by size: 2229 1545 1004 526 288 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 2517 4588 2517 Size: 2071 3618 PflMI CCAn_nnn'nTGG Cuts at: 1108 2716 1108 Size: 1608 4081 PfoI T'CCnGG_A Cuts at: 2250 2586 2715 2250 Size: 336 129 5224 Fragments arranged by size: 5224 336 129 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PmlI CAC'GTG Cuts at: 2500 2500 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2114 2146 3758 3790 5271 5303 2114 Size: 32 1612 32 1481 32 2500 Fragments arranged by size: 2500 1612 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2713 2920 2713 Size: 207 5482 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PspOMI G'GGCC_C Cuts at: 2174 2755 2174 Size: 581 5108 PstI C_TGCA'G Cuts at: 830 2379 3041 830 Size: 1549 662 3478 Fragments arranged by size: 3478 1549 662 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 SacI G_AGCT'C Cuts at: 2247 2247 Size: 5689 SacII CC_GC'GG Cuts at: 2202 2202 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SanDI GG'GwC_CC Cuts at: 2920 2920 Size: 5689 SbfI CC_TGCA'GG Cuts at: 2379 3041 2379 Size: 662 5027 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SexAI A'CCwGG_T Cuts at: 2709 2709 Size: 5689 SfcI C'TryA_G Cuts at: 826 1045 2375 3037 4172 4853 5044 826 Size: 219 1330 662 1135 681 191 1471 Fragments arranged by size: 1471 1330 1135 681 662 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2213 2213 Size: 5689 SfoI GGC'GCC Cuts at: 2224 2434 2224 Size: 210 5479 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 2358 3016 1872 Size: 486 658 4545 Fragments arranged by size: 4545 658 486 SmlI C'TyrA_G Cuts at: 820 1017 1943 2555 3793 4694 4956 820 Size: 197 926 612 1238 901 262 1553 Fragments arranged by size: 1553 1238 926 901 612 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 2233 3047 1132 Size: 1101 814 3774 Fragments arranged by size: 3774 1101 814 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 2484 1517 Size: 967 4722 StyI C'CwwG_G Cuts at: 513 1065 2641 3128 513 Size: 552 1576 487 3074 Fragments arranged by size: 3074 1576 552 487 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2990 3716 3733 3886 4071 4516 325 Size: 1255 1410 726 17 153 185 445 1498 Fragments arranged by size: 1498 1410 1255 726 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2811 3935 5647 Size: 274 80 33 51 1443 834 1124 1712 Cuts at: 5647 96 Size: 138 Fragments arranged by size: 1712 1443 1124 834 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2345 2377 2472 2504 2759 2791 2849 2881 2912 Size: 32 95 32 255 32 58 32 31 Cuts at: 2912 2944 5649 5681 2345 Size: 32 2705 32 2353 Fragments arranged by size: 2705 2353 255 95 58 32 32 32 32 32 32 31 Tth111I GACn'n_nGTC Cuts at: 1316 2711 1316 Size: 1395 4294 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2518 2518 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 2356 3014 1870 Size: 486 658 4545 Fragments arranged by size: 4545 658 486 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI PvuII RsrII SapI SrfI SwaI