(Linear) (Six-base) MAPSORT of: KIEE4326.seq Check: 6326 from: 1 to: 984 KIEE4326.orf With 182 enzymes: SgfI * March 22, 2012 17:44 .. AccI GT'mk_AC Cuts at: 0 308 984 Size: 308 676 AflIII A'CryG_T Cuts at: 0 512 984 Size: 512 472 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 109 141 984 Size: 109 32 843 Fragments arranged by size: 843 109 32 AlwNI CAG_nnn'CTG Cuts at: 0 333 378 699 711 984 Size: 333 45 321 12 273 Fragments arranged by size: 333 321 273 45 12 ApaI G_GGCC'C Cuts at: 0 173 754 984 Size: 173 581 230 Fragments arranged by size: 581 230 173 ApaLI G'TGCA_C Cuts at: 0 964 984 Size: 964 20 AvaI C'yCGr_G Cuts at: 0 148 351 919 984 Size: 148 203 568 65 Fragments arranged by size: 568 203 148 65 BanI G'GyrC_C Cuts at: 0 217 427 984 Size: 217 210 557 Fragments arranged by size: 557 217 210 BanII G_rGCy'C Cuts at: 0 173 242 358 653 754 900 984 Size: 173 69 116 295 101 146 84 Fragments arranged by size: 295 173 146 116 101 84 69 BbeI G_GCGC'C Cuts at: 0 221 431 984 Size: 221 210 553 Fragments arranged by size: 553 221 210 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 937 971 984 Size: 937 34 13 Fragments arranged by size: 937 34 13 BglI GCCn_nnn'nGGC Cuts at: 0 199 208 984 Size: 199 9 776 Fragments arranged by size: 776 199 9 Bme1580I G_kGCm'C Cuts at: 0 173 397 754 968 984 Size: 173 224 357 214 16 Fragments arranged by size: 357 224 214 173 16 BmrI ACTGGGnnnn_n' Cuts at: 0 91 984 Size: 91 893 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 738 984 Size: 738 246 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 664 733 778 984 Size: 307 357 69 45 206 Fragments arranged by size: 357 307 206 69 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 535 984 Size: 535 449 BsaAI yAC'GTr Cuts at: 0 495 984 Size: 495 489 BsaHI Gr'CG_yC Cuts at: 0 218 305 416 428 984 Size: 218 87 111 12 556 Fragments arranged by size: 556 218 111 87 12 BsaWI w'CCGG_w Cuts at: 0 738 984 Size: 738 246 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 109 139 340 370 467 497 754 784 Size: 109 30 201 30 97 30 257 30 Cuts at: 784 844 874 984 Size: 60 30 110 Fragments arranged by size: 257 201 110 109 97 60 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 661 984 Size: 661 323 BseYI C'CCAG_C Cuts at: 0 971 984 Size: 971 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 126 760 984 Size: 126 634 224 Fragments arranged by size: 634 224 126 BsiHKAI G_wGCw'C Cuts at: 0 242 764 968 984 Size: 242 522 204 16 Fragments arranged by size: 522 242 204 16 Bsp1286I G_dGCh'C Cuts at: 0 173 242 358 397 653 754 764 900 Size: 173 69 116 39 256 101 10 136 Cuts at: 900 968 984 Size: 68 16 Fragments arranged by size: 256 173 136 116 101 69 68 39 16 10 BspEI T'CCGG_A Cuts at: 0 738 984 Size: 738 246 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 432 984 Size: 432 552 BsrBI CCG'CTC Cuts at: 0 717 747 984 Size: 717 30 237 Fragments arranged by size: 717 237 30 BsrFI r'CCGG_y Cuts at: 0 177 605 984 Size: 177 428 379 Fragments arranged by size: 428 379 177 BsrGI T'GTAC_A Cuts at: 0 806 984 Size: 806 178 BssHII G'CGCG_C Cuts at: 0 109 111 984 Size: 109 2 873 Fragments arranged by size: 873 109 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 230 984 Size: 230 754 BstYI r'GATC_y Cuts at: 0 719 984 Size: 719 265 BtgI C'CryG_G Cuts at: 0 194 984 Size: 194 790 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 473 984 Size: 473 511 BtsI GCAGTG_nn' Cuts at: 0 212 537 984 Size: 212 325 447 Fragments arranged by size: 447 325 212 EaeI y'GGCC_r Cuts at: 0 158 200 209 324 537 572 984 Size: 158 42 9 115 213 35 412 Fragments arranged by size: 412 213 158 115 42 35 9 EarI CTCTTCn'nnn_ Cuts at: 0 937 984 Size: 937 47 EcoICRI GAG'CTC Cuts at: 0 240 984 Size: 240 744 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 664 733 778 984 Size: 307 357 69 45 206 Fragments arranged by size: 357 307 206 69 45 EcoO109I rG'GnC_Cy Cuts at: 0 170 708 750 751 915 984 Size: 170 538 42 1 164 69 Fragments arranged by size: 538 170 164 69 42 1 HaeII r_GCGC'y Cuts at: 0 156 221 379 411 431 984 Size: 156 65 158 32 20 553 Fragments arranged by size: 553 158 156 65 32 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 451 483 984 Size: 451 32 501 Fragments arranged by size: 501 451 32 KasI G'GCGC_C Cuts at: 0 217 427 984 Size: 217 210 557 Fragments arranged by size: 557 217 210 MslI CAynn'nnrTG Cuts at: 0 511 984 Size: 511 473 MspA1I CmG'CkG Cuts at: 0 33 48 57 66 131 196 975 984 Size: 33 15 9 9 65 65 779 9 Fragments arranged by size: 779 65 65 33 15 9 9 9 NarI GG'CG_CC Cuts at: 0 218 428 984 Size: 218 210 556 Fragments arranged by size: 556 218 210 NspI r_CATG'y Cuts at: 0 228 516 984 Size: 228 288 468 Fragments arranged by size: 468 288 228 PciI A'CATG_T Cuts at: 0 512 984 Size: 512 472 PflMI CCAn_nnn'nTGG Cuts at: 0 711 984 Size: 711 273 PfoI T'CCnGG_A Cuts at: 0 245 581 710 984 Size: 245 336 129 274 Fragments arranged by size: 336 274 245 129 PmlI CAC'GTG Cuts at: 0 495 984 Size: 495 489 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 109 141 984 Size: 109 32 843 Fragments arranged by size: 843 109 32 PpuMI rG'GwC_Cy Cuts at: 0 708 915 984 Size: 708 207 69 Fragments arranged by size: 708 207 69 PspOMI G'GGCC_C Cuts at: 0 169 750 984 Size: 169 581 234 Fragments arranged by size: 581 234 169 PstI C_TGCA'G Cuts at: 0 374 984 Size: 374 610 SacI G_AGCT'C Cuts at: 0 242 984 Size: 242 742 SacII CC_GC'GG Cuts at: 0 197 984 Size: 197 787 SanDI GG'GwC_CC Cuts at: 0 915 984 Size: 915 69 SbfI CC_TGCA'GG Cuts at: 0 374 984 Size: 374 610 SexAI A'CCwGG_T Cuts at: 0 704 984 Size: 704 280 SfcI C'TryA_G Cuts at: 0 370 984 Size: 370 614 SfiI GGCCn_nnn'nGGCC Cuts at: 0 208 984 Size: 208 776 SfoI GGC'GCC Cuts at: 0 219 429 984 Size: 219 210 555 Fragments arranged by size: 555 219 210 SmaI CCC'GGG Cuts at: 0 353 984 Size: 353 631 SmlI C'TyrA_G Cuts at: 0 550 984 Size: 550 434 SphI G_CATG'C Cuts at: 0 228 984 Size: 228 756 StuI AGG'CCT Cuts at: 0 479 984 Size: 479 505 StyI C'CwwG_G Cuts at: 0 636 984 Size: 636 348 TatI w'GTAC_w Cuts at: 0 806 984 Size: 806 178 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 340 372 467 499 754 786 844 876 Size: 340 32 95 32 255 32 58 32 Cuts at: 876 907 939 984 Size: 31 32 45 Fragments arranged by size: 340 255 95 58 45 32 32 32 32 32 31 Tth111I GACn'n_nGTC Cuts at: 0 706 984 Size: 706 278 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 513 984 Size: 513 471 XmaI C'CCGG_G Cuts at: 0 351 984 Size: 351 633 Enzymes that do cut: AccI AflIII AloI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BcgI BglI Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstYI BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MslI MspA1I NarI NspI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI SacI SacII SanDI SbfI SexAI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmtI Bpu10I BsaI BsaBI BsiEI BsiWI BsmI BsmBI BspHI BsrDI BssSI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI SapI ScaI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TaqII XbaI XhoI XmnI ZraI