################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AvaII AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BssHII BstAPI BstEII BstPI BstUI CelII CfoI ClaI CpoI DraII DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NcoI NgoMIV NotI NruI PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AciI 88 CCGC CCGC(-3/-1) AgeI 1049 ACCGGT A^CCGGT AhdI 779 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 331 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 779 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1309 GAGCTC G[AT]GC[AT]^C BalI 918 TGGCCA TGG^CCA BamHI 20 GGATCC G^GATCC BanII 1309 GAGCTC G[GA]GC[CT]^C BcnI 126 CCGGG CC^[GC]GG BmyI 1309 GAGCTC G[AGT]GC[ACT]^C BsaJI 126 CCGGGG C^C[ACGT][ACGT]GG BsaWI 1049 ACCGGT [AT]^CCGG[AT] BsiHKAI 1309 GAGCTC G[AT]GC[AT]^C BsmAI 1517 GTCTC GTCTC(1/5) Bsp1286I 1309 GAGCTC G[AGT]GC[ACT]^C BsrDI 1314 CATTGC GCAATG(2/0) BsrFI 1049 ACCGGT [GA]^CCGG[CT] BssSI 109 CTCGTG CACGAG(-5/-1) Bst1107I 468 GTATAC GTA^TAC BstBI 880 TTCGAA TT^CGAA BstZ17I 468 GTATAC GTA^TAC Cfr10I 1049 ACCGGT [GA]^CCGG[CT] Cfr13I 68 GGCCC G^G[ACGT]CC EaeI 918 TGGCCA [CT]^GGCC[GA] Eam1105I 779 GACACAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1306 GAAGAG CTCTTC(1/4) EcoT22I 618 ATGCAT ATGCA^T HgaI 408 GCGTC GACGC(5/10) HindIII 400 AAGCTT A^AGCTT Ksp632I 1306 GAAGAG CTCTTC(1/4) MaeII 215 ACGT A^CGT MfeI 1172 CAATTG C^AATTG MluNI 918 TGGCCA TGG^CCA MscI 918 TGGCCA TGG^CCA MslI 568 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 76 CAGCTG C[AC]G^C[GT]G MunI 1172 CAATTG C^AATTG NciI 126 CCGGG CC^[GC]GG NdeI 1025 CATATG CA^TATG NheI 927 GCTAGC G^CTAGC NlaIV 20 GGATCC GG[ACGT]^[ACGT]CC NsiI 618 ATGCAT ATGCA^T NspI 90 GCATGT [GA]CATG^[CT] NspV 880 TTCGAA TT^CGAA PflMI 456 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1049 ACCGGT A^CCGGT PleI 107 GACTC GAGTC(4/5) PstI 118 CTGCAG CTGCA^G PvuII 76 CAGCTG CAG^CTG SacI 1309 GAGCTC GAGCT^C SapI 1306 GAAGAGC GCTCTTC(1/4) Sau96I 68 GGCCC G^G[ACGT]CC SexAI 274 ACCAGGT A^CC[AT]GGT SfuI 880 TTCGAA TT^CGAA SpeI 154 ACTAGT A^CTAGT Tsp45I 151 GTGAC ^GT[GC]AC Van91I 456 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 331 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 426 GTCTAC GT^[AC][GT]AC 468 GTATAC AcsI 1114 AAATTT [GA]^AATT[CT] 1330 GAATTT AocI 56 CCTCAGG CC^T[ACGT]AGG 478 CCTCAGG ApoI 1114 AAATTT [GA]^AATT[CT] 1330 GAATTT BclI 95 TGATCA T^GATCA 1335 TGATCA BpmI 7 CTGGAG CTGGAG(16/14) 755 CTGGAG BseRI 476 CTCCTC GAGGAG(10/8) 10 GAGGAG BsmFI 105 GGGAC GGGAC(10/14) 611 GTCCC Bsp1407I 1404 TGTACA T^GTACA 1507 TGTACA BsrGI 1404 TGTACA T^GTACA 1507 TGTACA BstXI 71 CCACGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1461 CCAAGAAAATGG BstYI 20 GGATCC [GA]^GATC[CT] 594 AGATCC Bsu36I 56 CCTCAGG CC^T[ACGT]AGG 478 CCTCAGG DdeI 57 CTCAG C^T[ACGT]AG 479 CTCAG DraI 1138 TTTAAA TTT^AAA 1423 TTTAAA Eco81I 56 CCTCAGG CC^T[ACGT]AGG 478 CCTCAGG FbaI 95 TGATCA T^GATCA 1335 TGATCA HaeIII 68 GGCC GG^CC 919 GGCC HapII 126 CCGG C^CGG 1050 CCGG HpaII 126 CCGG C^CGG 1050 CCGG MaeIII 151 GTGAC ^GT[ACGT]AC 459 GTTAC MflI 20 GGATCC [GA]^GATC[CT] 594 AGATCC MspI 126 CCGG C^CGG 1050 CCGG SfaNI 949 GATGC GCATC(5/9) 50 GCATC SfcI 118 CTGCAG C^T[GA][CT]AG 1535 CTGTAG SspBI 1404 TGTACA T^GTACA 1507 TGTACA XhoII 20 GGATCC [GA]^GATC[CT] 594 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AfaI AluI AlwI AlwNI BbsI BbvI BfaI BpuAI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I DpnII EcoRII Fnu4HI FokI HinfI HphI ItaI MaeI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I