(Circular) (Six-base) MAPSORT of: pFN21AE1388.seq Check: 3504 from: 1 to: 6256 pFN21AE1388 6256 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2432 2474 3599 1937 Size: 495 42 1125 4594 Fragments arranged by size: 4594 1125 495 42 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2250 2688 2841 4319 5703 5971 1632 Size: 618 438 153 1478 1384 268 1917 Fragments arranged by size: 1917 1478 1384 618 438 268 153 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 5155 6132 1203 Size: 3952 977 1327 Fragments arranged by size: 3952 1327 977 AgeI A'CCGG_T Cuts at: 3054 5087 3054 Size: 2033 4223 AhdI GACnn_n'nnGTC Cuts at: 2789 4985 2789 Size: 2196 4060 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6256 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6256 AlwNI CAG_nnn'CTG Cuts at: 2467 2648 2984 3999 5571 2467 Size: 181 336 1015 1572 3152 Fragments arranged by size: 3152 1572 1015 336 181 ApaLI G'TGCA_C Cuts at: 4313 5469 4313 Size: 1156 5100 ApoI r'AATT_y Cuts at: 1493 3119 3335 3565 3791 3943 6052 1493 Size: 1626 216 230 226 152 2109 1697 Fragments arranged by size: 2109 1697 1626 230 226 216 152 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 3581 1870 Size: 73 1638 4545 Fragments arranged by size: 4545 1638 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2905 2938 2905 Size: 33 6223 BamHI G'GATC_C Cuts at: 2025 3586 3953 2025 Size: 1561 367 4328 Fragments arranged by size: 4328 1561 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3577 5032 618 Size: 325 217 627 1790 1455 1842 Fragments arranged by size: 1842 1790 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3318 3575 1391 Size: 1927 257 4072 Fragments arranged by size: 4072 1927 257 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2238 2694 2895 928 Size: 971 339 456 201 4289 Fragments arranged by size: 4289 971 456 339 201 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4447 4481 1903 Size: 34 2510 34 3678 Fragments arranged by size: 3678 2510 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4148 5358 4148 Size: 1210 5046 BclI T'GATC_A Cuts at: 1548 2100 3340 1548 Size: 552 1240 4464 Fragments arranged by size: 4464 1240 552 BfrBI ATG'CAT Cuts at: 2625 2625 Size: 6256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4867 136 Size: 107 122 71 631 605 3195 1525 Fragments arranged by size: 3195 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6251 6251 Size: 6256 BlpI GC'TnA_GC Cuts at: 3673 3673 Size: 6256 Bme1580I G_kGCm'C Cuts at: 1790 4317 5473 1790 Size: 2527 1156 2573 Fragments arranged by size: 2573 2527 1156 BmgBI CAC'GTC Cuts at: 1318 2221 1318 Size: 903 5353 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2455 2518 2825 Size: 416 53 83 399 24 1038 63 307 Cuts at: 2825 3987 4945 442 Size: 1162 958 1753 Fragments arranged by size: 1753 1162 1038 958 416 399 307 83 63 53 24 BmtI G_CTAG'C Cuts at: 1056 2936 1056 Size: 1880 4376 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2781 4916 1239 Size: 794 748 2135 2579 Fragments arranged by size: 2579 2135 794 748 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4381 5246 5544 4381 Size: 865 298 5093 Fragments arranged by size: 5093 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4919 882 Size: 617 3420 2219 Fragments arranged by size: 3420 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4047 493 Size: 711 2843 2702 Fragments arranged by size: 2843 2702 711 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4063 4445 5080 275 Size: 53 83 186 936 2530 382 635 1451 Fragments arranged by size: 2530 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3054 4689 5087 5361 5508 5919 3054 Size: 1635 398 274 147 411 3391 Fragments arranged by size: 3391 1635 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2754 2784 3029 3059 1178 Size: 30 165 30 1351 30 245 30 4375 Fragments arranged by size: 4375 1351 245 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2472 1186 Size: 844 442 4970 Fragments arranged by size: 4970 844 442 BseYI C'CCAG_C Cuts at: 5459 5955 5459 Size: 496 5760 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5683 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3706 4467 5495 1540 Size: 275 186 1705 761 1028 2301 Fragments arranged by size: 2301 1705 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3318 4317 4402 5473 3318 Size: 999 85 1071 4101 Fragments arranged by size: 4101 1071 999 85 BsmI GAATG_Cn' Cuts at: 3767 3860 3767 Size: 93 6163 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3318 3575 4317 4402 5473 1391 Size: 399 1528 257 742 85 1071 2174 Fragments arranged by size: 2174 1528 1071 742 399 257 85 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3611 844 Size: 2767 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4143 1394 Size: 429 2320 3507 Fragments arranged by size: 3507 2320 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3318 4751 4925 65 Size: 1183 2070 1433 174 1396 Fragments arranged by size: 2070 1433 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3054 4900 5087 1700 Size: 110 1244 1846 187 2869 Fragments arranged by size: 2869 1846 1244 187 110 BsrGI T'GTAC_A Cuts at: 96 3409 3512 96 Size: 3313 103 2840 Fragments arranged by size: 3313 2840 103 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6256 BssSI C'ACGA_G Cuts at: 2114 4316 5328 2114 Size: 2202 1012 3042 Fragments arranged by size: 3042 2202 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 2886 5060 2886 Size: 2174 4082 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6256 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2083 3473 2011 Size: 72 1390 4794 Fragments arranged by size: 4794 1390 72 BstYI r'GATC_y Cuts at: 1972 2025 2599 3586 3953 4338 4355 5807 5893 Size: 53 574 987 367 385 17 1452 86 Cuts at: 5893 5905 6251 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 987 574 385 367 346 86 53 17 12 BstZ17I GTA'TAC Cuts at: 2475 2475 Size: 6256 Bsu36I CC'TnA_GG Cuts at: 2062 2484 2062 Size: 422 5834 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4765 Fragments arranged by size: 4765 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2041 487 Size: 683 56 815 4702 Fragments arranged by size: 4702 815 683 56 BtsI GCAGTG_nn' Cuts at: 3772 4566 4586 3772 Size: 794 20 5442 Fragments arranged by size: 5442 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 3145 3430 3560 3145 Size: 285 130 5841 Fragments arranged by size: 5841 285 130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2923 3703 4592 Size: 54 1425 50 275 18 1093 780 889 Cuts at: 4592 6221 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1093 889 780 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3703 1537 Size: 275 1891 4090 Fragments arranged by size: 4090 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3306 4186 1353 Size: 1953 880 3423 Fragments arranged by size: 3423 1953 880 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4843 5217 5363 387 Size: 1064 138 3254 374 146 1280 Fragments arranged by size: 3254 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3316 3316 Size: 6256 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2250 2688 2781 2841 4319 4916 Size: 393 401 217 438 93 60 1478 597 Cuts at: 4916 5703 5971 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1478 787 597 438 401 393 268 217 93 60 EcoRI G'AATT_C Cuts at: 3565 3943 3565 Size: 378 5878 FspI TGC'GCA Cuts at: 4762 4762 Size: 6256 HaeII r_GCGC'y Cuts at: 1678 5403 1678 Size: 3725 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2775 2795 2807 2827 4897 Size: 32 135 32 1203 20 12 20 2070 Cuts at: 4897 4929 4971 5003 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2070 1203 135 42 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 3600 3846 669 Size: 988 281 1662 246 3079 Fragments arranged by size: 3079 1662 988 281 246 HindIII A'AGCT_T Cuts at: 748 2405 748 Size: 1657 4599 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3177 3855 3177 Size: 678 5578 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5370 5554 1305 Size: 4065 184 2007 Fragments arranged by size: 4065 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2925 10 Size: 54 1425 343 1093 3341 Fragments arranged by size: 3341 1425 1093 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2577 4214 4573 4732 518 Size: 648 1411 1637 359 159 2042 Fragments arranged by size: 2042 1637 1411 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2083 3672 4349 5497 5742 1606 Size: 477 1589 677 1148 245 2120 Fragments arranged by size: 2120 1589 1148 677 477 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4765 Fragments arranged by size: 4765 939 552 NdeI CA'TA_TG Cuts at: 387 3031 387 Size: 2644 3612 NheI G'CTAG_C Cuts at: 1052 2932 1052 Size: 1880 4376 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NsiI A_TGCA'T Cuts at: 2627 2627 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 2099 3614 5159 1132 Size: 97 870 1515 1545 2229 Fragments arranged by size: 2229 1545 1515 870 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6256 PciI A'CATG_T Cuts at: 5155 5155 Size: 6256 PflMI CCAn_nnn'nTGG Cuts at: 1108 2467 1108 Size: 1359 4897 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 2567 3826 2567 Size: 1259 4997 PstI C_TGCA'G Cuts at: 830 2127 3608 830 Size: 1297 1481 3478 Fragments arranged by size: 3478 1481 1297 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 PvuII CAG'CTG Cuts at: 2083 2083 Size: 6256 SacI G_AGCT'C Cuts at: 3318 3318 Size: 6256 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SapI GCTCTTCn'nnn_ Cuts at: 3306 3306 Size: 6256 SbfI CC_TGCA'GG Cuts at: 3608 3608 Size: 6256 ScaI AGT'ACT Cuts at: 4504 6216 4504 Size: 1712 4544 SexAI A'CCwGG_T Cuts at: 2279 2279 Size: 6256 SfcI C'TryA_G Cuts at: 826 1045 2123 3540 3604 4739 5420 5611 826 Size: 219 1078 1417 64 1135 681 191 1471 Fragments arranged by size: 1471 1417 1135 1078 681 219 191 64 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 3583 1872 Size: 1711 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4360 5261 5523 820 Size: 197 926 2417 901 262 1553 Fragments arranged by size: 2417 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 2159 152 Size: 2007 4249 SphI G_CATG'C Cuts at: 1132 3614 1132 Size: 2482 3774 SspI AAT'ATT Cuts at: 5 52 4180 5 Size: 47 4128 2081 Fragments arranged by size: 4128 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6256 StyI C'CwwG_G Cuts at: 513 1065 2004 3695 513 Size: 552 939 1691 3074 Fragments arranged by size: 3074 1691 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2560 2683 4283 4300 4453 4638 5083 Size: 1255 980 123 1600 17 153 185 445 Cuts at: 5083 325 Size: 1498 Fragments arranged by size: 1600 1498 1255 980 445 185 153 123 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3409 3512 4502 Size: 274 80 33 51 1443 1432 103 990 Cuts at: 4502 6214 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1432 990 274 138 103 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2682 2714 2754 2786 2817 2849 3029 3061 6216 Size: 32 40 32 31 32 180 32 3155 Cuts at: 6216 6248 2682 Size: 32 2690 Fragments arranged by size: 3155 2690 180 40 32 32 32 32 32 31 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6256 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6256 XmaI C'CCGG_G Cuts at: 1870 3581 1870 Size: 1711 4545 XmnI GAAnn'nnTTC Cuts at: 2340 4385 2340 Size: 2045 4211 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI