Restriction map for
pFN21AE1388
(Linear) (Six-base) MAPSORT of: KIEE1388.seq Check: 5047 from: 1 to: 1551
KIEE1388.orf
With 182 enzymes: SgfI *
July 15, 2010 18:37 ..
AccI GT'mk_AC
Cuts at: 0 427 469 1551
Size: 427 42 1082
Fragments arranged by size:
1082 427 42
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 683 836 1551
Size: 245 438 153 715
Fragments arranged by size:
715 438 245 153
AgeI A'CCGG_T
Cuts at: 0 1049 1551
Size: 1049 502
AhdI GACnn_n'nnGTC
Cuts at: 0 784 1551
Size: 784 767
AlwNI CAG_nnn'CTG
Cuts at: 0 462 643 979 1551
Size: 462 181 336 572
Fragments arranged by size:
572 462 336 181
ApoI r'AATT_y
Cuts at: 0 1114 1330 1551
Size: 1114 216 221
Fragments arranged by size:
1114 221 216
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 900 933 1551
Size: 900 33 618
Fragments arranged by size:
900 618 33
BamHI G'GATC_C
Cuts at: 0 20 1551
Size: 20 1531
BanII G_rGCy'C
Cuts at: 0 1313 1551
Size: 1313 238
BbsI GAAGACnn'nnnn_
Cuts at: 0 233 689 890 1551
Size: 233 456 201 661
Fragments arranged by size:
661 456 233 201
BclI T'GATC_A
Cuts at: 0 95 1335 1551
Size: 95 1240 216
Fragments arranged by size:
1240 216 95
BfrBI ATG'CAT
Cuts at: 0 620 1551
Size: 620 931
BmgBI CAC'GTC
Cuts at: 0 216 1551
Size: 216 1335
BmrI ACTGGGnnnn_n'
Cuts at: 0 450 513 820 1551
Size: 450 63 307 731
Fragments arranged by size:
731 450 307 63
BmtI G_CTAG'C
Cuts at: 0 931 1551
Size: 931 620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 776 1551
Size: 28 748 775
Fragments arranged by size:
775 748 28
BsaWI w'CCGG_w
Cuts at: 0 1049 1551
Size: 1049 502
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 749 779 1024 1054 1551
Size: 749 30 245 30 497
Fragments arranged by size:
749 497 245 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 467 1551
Size: 25 442 1084
Fragments arranged by size:
1084 442 25
BsiHKAI G_wGCw'C
Cuts at: 0 1313 1551
Size: 1313 238
Bsp1286I G_dGCh'C
Cuts at: 0 1313 1551
Size: 1313 238
BsrDI GCAATG_nn'
Cuts at: 0 1313 1551
Size: 1313 238
BsrFI r'CCGG_y
Cuts at: 0 1049 1551
Size: 1049 502
BsrGI T'GTAC_A
Cuts at: 0 1404 1507 1551
Size: 1404 103 44
Fragments arranged by size:
1404 103 44
BssSI C'ACGA_G
Cuts at: 0 109 1551
Size: 109 1442
BstBI TT'CG_AA
Cuts at: 0 881 1551
Size: 881 670
BstXI CCAn_nnnn'nTGG
Cuts at: 0 78 1468 1551
Size: 78 1390 83
Fragments arranged by size:
1390 83 78
BstYI r'GATC_y
Cuts at: 0 20 594 1551
Size: 20 574 957
Fragments arranged by size:
957 574 20
BstZ17I GTA'TAC
Cuts at: 0 470 1551
Size: 470 1081
Bsu36I CC'TnA_GG
Cuts at: 0 57 479 1551
Size: 57 422 1072
Fragments arranged by size:
1072 422 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 36 1551
Size: 36 1515
DraI TTT'AAA
Cuts at: 0 1140 1425 1551
Size: 1140 285 126
Fragments arranged by size:
1140 285 126
EaeI y'GGCC_r
Cuts at: 0 918 1551
Size: 918 633
EarI CTCTTCn'nnn_
Cuts at: 0 1301 1551
Size: 1301 250
EcoICRI GAG'CTC
Cuts at: 0 1311 1551
Size: 1311 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 245 683 776 836 1551
Size: 28 217 438 93 60 715
Fragments arranged by size:
715 438 217 93 60 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 770 790 802 822 1551
Size: 770 20 12 20 729
Fragments arranged by size:
770 729 20 20 12
HindIII A'AGCT_T
Cuts at: 0 400 1551
Size: 400 1151
MfeI C'AATT_G
Cuts at: 0 1172 1551
Size: 1172 379
MscI TGG'CCA
Cuts at: 0 920 1551
Size: 920 631
MslI CAynn'nnrTG
Cuts at: 0 572 1551
Size: 572 979
MspA1I CmG'CkG
Cuts at: 0 78 1551
Size: 78 1473
NdeI CA'TA_TG
Cuts at: 0 1026 1551
Size: 1026 525
NheI G'CTAG_C
Cuts at: 0 927 1551
Size: 927 624
NsiI A_TGCA'T
Cuts at: 0 622 1551
Size: 622 929
NspI r_CATG'y
Cuts at: 0 94 1551
Size: 94 1457
PflMI CCAn_nnn'nTGG
Cuts at: 0 462 1551
Size: 462 1089
PsiI TTA'TAA
Cuts at: 0 562 1551
Size: 562 989
PstI C_TGCA'G
Cuts at: 0 122 1551
Size: 122 1429
PvuII CAG'CTG
Cuts at: 0 78 1551
Size: 78 1473
SacI G_AGCT'C
Cuts at: 0 1313 1551
Size: 1313 238
SapI GCTCTTCn'nnn_
Cuts at: 0 1301 1551
Size: 1301 250
SexAI A'CCwGG_T
Cuts at: 0 274 1551
Size: 274 1277
SfcI C'TryA_G
Cuts at: 0 118 1535 1551
Size: 118 1417 16
Fragments arranged by size:
1417 118 16
SpeI A'CTAG_T
Cuts at: 0 154 1551
Size: 154 1397
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 555 678 1551
Size: 555 123 873
Fragments arranged by size:
873 555 123
TatI w'GTAC_w
Cuts at: 0 1404 1507 1551
Size: 1404 103 44
Fragments arranged by size:
1404 103 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 677 709 749 781 812 844 1024 1056
Size: 677 32 40 32 31 32 180 32
Cuts at: 1056 1551
Size: 495
Fragments arranged by size:
677 495 180 40 32 32 32 32
31
XmnI GAAnn'nnTTC
Cuts at: 0 335 1551
Size: 335 1216
Enzymes that do cut:
AccI AcuI AgeI AhdI AlwNI ApoI BaeI BamHI
BanII BbsI BclI BfrBI BmgBI BmrI BmtI BpmI
BsaWI BsaXI BseRI BsiHKAI Bsp1286I BsrDI BsrFI BsrGI
BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI DraI
EaeI EarI EcoICRI Eco57MI Hin4I HindIII MfeI MscI
MslI MspA1I NdeI NheI NsiI NspI PflMI PsiI
PstI PvuII SacI SapI SexAI SfcI SpeI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BanI BbeI BbvCI BcgI BciVI BglI BglII
BlpI Bme1580I BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BssHII BstAPI BstEII BtgI BtsI
ClaI CspCI DraIII DrdI EagI EciI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MluI MmeI NaeI NarI NcoI
NgoMIV NotI NruI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI
StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI