################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI StyI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 179 GGTACC G^GTACC AciI 367 CCGC CCGC(-3/-1) AlwI 201 GATCC GGATC(4/5) AlwNI 500 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 179 GGTACC G^GTACC AspHI 104 GAGCTC G[AT]GC[AT]^C BanI 179 GGTACC G^G[CT][GA]CC BanII 104 GAGCTC G[GA]GC[CT]^C BmyI 104 GAGCTC G[AGT]GC[ACT]^C BpmI 513 CTCCAG CTGGAG(16/14) BsaI 294 GAGACC GGTCTC(1/5) BsaJI 57 CCCAGG C^C[ACGT][ACGT]GG BseRI 194 CTCCTC GAGGAG(10/8) BsgI 31 CTGCAC GTGCAG(16/14) BsiHKAI 104 GAGCTC G[AT]GC[AT]^C BsmAI 294 GAGAC GTCTC(1/5) BsmI 423 GAATGC GAATGC(1/-1) Bsp1286I 104 GAGCTC G[AGT]GC[ACT]^C BspMI 98 ACCTGC ACCTGC(4/8) BsrBI 367 CCGCTC CCGCTC(-3/-3) BsrI 548 CCAGT ACTGG(1/-1) BssKI 58 CCAGG ^CC[ACGT]GG BstNI 58 CCAGG CC^[AT]GG BstYI 200 AGATCC [GA]^GATC[CT] DpnII 201 GATC ^GATC DraI 394 TTTAAA TTT^AAA EcoRII 58 CCAGG ^CC[AT]GG FauI 366 CCCGC CCCGC(4/6) HaeIII 546 GGCC GG^CC HincII 321 GTTGAC GT[CT]^[GA]AC HindII 321 GTTGAC GT[CT]^[GA]AC HphI 508 GGTGA GGTGA(8/7) KpnI 179 GGTACC GGTAC^C MaeII 15 ACGT A^CGT MaeIII 509 GTGAC ^GT[ACGT]AC MboI 201 GATC ^GATC MflI 200 AGATCC [GA]^GATC[CT] MslI 580 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 334 CAGCTG C[AC]G^C[GT]G MvaI 58 CCAGG CC^[AT]GG NdeII 201 GATC ^GATC PleI 511 GACTC GAGTC(4/5) PvuII 334 CAGCTG CAG^CTG SacI 104 GAGCTC GAGCT^C Sau3AI 201 GATC ^GATC ScrFI 58 CCAGG CC^[ACGT]GG SmlI 418 CTCAAG C^T[CT][GA]AG SphI 447 GCATGC GCATG^C SspI 111 AATATT AAT^ATT SwaI 393 ATTTAAAT ATTT^AAAT TfiI 238 GATTC G^A[AT]TC Tsp45I 509 GTGAC ^GT[GC]AC TspRI 433 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 280 CCAAAAGGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 200 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 391 GAATTT [GA]^AATT[CT] 574 AAATTT AfaI 180 GTAC GT^AC 520 GTAC AflIII 35 ACATGT A^C[GA][CT]GT 67 ACATGT ApoI 391 GAATTT [GA]^AATT[CT] 574 AAATTT BbvI 30 GCTGC GCAGC(8/12) 336 GCTGC BfaI 157 CTAG C^TAG 556 CTAG BspLU11I 35 ACATGT A^CATGT 67 ACATGT EarI 21 GAAGAG CTCTTC(1/4) 478 GAAGAG Fnu4HI 30 GCTGC GC^[ACGT]GC 336 GCTGC HinfI 238 GATTC G^A[ACGT]TC 511 GACTC ItaI 30 GCTGC GC^[ACGT]GC 336 GCTGC Ksp632I 21 GAAGAG CTCTTC(1/4) 478 GAAGAG MaeI 157 CTAG C^TAG 556 CTAG MboII 21 GAAGA GAAGA(8/7) 478 GAAGA MseI 152 TTAA T^TAA 395 TTAA NlaIV 121 GGAGCC GG[ACGT]^[ACGT]CC 179 GGTACC PflMI 428 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 499 CCAGAGGCTGG RsaI 180 GTAC GT^AC 520 GTAC SfaNI 483 GATGC GCATC(5/9) 62 GCATC Tru9I 152 TTAA T^TAA 395 TTAA TseI 30 GCTGC G^C[AT]GC 336 GCTGC Van91I 428 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 499 CCAGAGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AluI BsiYI BslI BstF5I Cac8I DdeI FokI MnlI NlaIII NspI Tsp509I