(Linear) (Six-base) MAPSORT of: KIEE3643.seq  Check: 3765  from: 1  to: 591



KIEE3643.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:37  ..

Acc65I G'GTAC_C

Cuts at:      0     179     591 
   Size:        179     412
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     591 
   Size:         40     551
 
AflIII A'CryG_T

Cuts at:      0      35      67     591 
   Size:         35      32     524

  Fragments arranged by size: 

                 524      35      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     505     591 
   Size:        505      86
 
ApoI r'AATT_y

Cuts at:      0     391     574     591 
   Size:        391     183      17

  Fragments arranged by size: 

                 391     183      17
 
BanI G'GyrC_C

Cuts at:      0     179     591 
   Size:        179     412
 
BanII G_rGCy'C

Cuts at:      0     108     591 
   Size:        108     483
 
BmgBI CAC'GTC

Cuts at:      0      16     591 
   Size:         16     575
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     498     591 
   Size:        498      93
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     591 
   Size:        403     188
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     288     591 
   Size:        288     303
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     185     591 
   Size:        185     406
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16     591 
   Size:         16     575
 
BsiHKAI G_wGCw'C

Cuts at:      0     108     591 
   Size:        108     483
 
BsmI GAATG_Cn'

Cuts at:      0     429     591 
   Size:        429     162
 
Bsp1286I G_dGCh'C

Cuts at:      0     108     591 
   Size:        108     483
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     107     591 
   Size:        107     484
 
BsrBI CCG'CTC

Cuts at:      0     369     591 
   Size:        369     222
 
BstYI r'GATC_y

Cuts at:      0     200     591 
   Size:        200     391
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     294     591 
   Size:        294     297
 
DraI TTT'AAA

Cuts at:      0     396     591 
   Size:        396     195
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     473     591 
   Size:         16     457     118

  Fragments arranged by size: 

                 457     118      16
 
EcoICRI GAG'CTC

Cuts at:      0     106     591 
   Size:        106     485
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     498     591 
   Size:         40     458      93

  Fragments arranged by size: 

                 458      93      40
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     429     502     591 
   Size:        429      73      89

  Fragments arranged by size: 

                 429      89      73
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     287     319     370     402     591 
   Size:        287      32      51      32     189

  Fragments arranged by size: 

                 287     189      51      32      32
 
HincII GTy'rAC

Cuts at:      0     323     591 
   Size:        323     268
 
KpnI G_GTAC'C

Cuts at:      0     183     591 
   Size:        183     408
 
MslI CAynn'nnrTG

Cuts at:      0     584     591 
   Size:        584       7
 
MspA1I CmG'CkG

Cuts at:      0     336     591 
   Size:        336     255
 
NspI r_CATG'y

Cuts at:      0      39      71     451     591 
   Size:         39      32     380     140

  Fragments arranged by size: 

                 380     140      39      32
 
PciI A'CATG_T

Cuts at:      0      35      67     591 
   Size:         35      32     524

  Fragments arranged by size: 

                 524      35      32
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     434     505     591 
   Size:        434      71      86

  Fragments arranged by size: 

                 434      86      71
 
PvuII CAG'CTG

Cuts at:      0     336     591 
   Size:        336     255
 
SacI G_AGCT'C

Cuts at:      0     108     591 
   Size:        108     483
 
SmlI C'TyrA_G

Cuts at:      0     418     591 
   Size:        418     173
 
SphI G_CATG'C

Cuts at:      0     451     591 
   Size:        451     140
 
SspI AAT'ATT

Cuts at:      0     113     591 
   Size:        113     478
 
SwaI ATTT'AAAT

Cuts at:      0     396     591 
   Size:        396     195
 
TatI w'GTAC_w

Cuts at:      0     519     591 
   Size:        519      72
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     287     591 
   Size:        287     304
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII    AlwNI     ApoI     BanI    BanII    BmgBI
     BpmI    BpuEI     BsaI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrBI    BstYI    BtgZI     DraI     EarI  EcoICRI  Eco57MI
     FalI    Hin4I   HincII     KpnI     MslI   MspA1I     NspI     PciI
    PflMI    PvuII     SacI     SmlI     SphI     SspI     SwaI     TatI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     StuI     StyI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI