################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BalI BamHI BanII BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BsaAI BsaBI BsaI BsgI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI BspDI BspHI BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI DsaI Eco47III EcoO65I EcoRI EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccIII 937 TCCGGA T^CCGGA AcyI 250 GGCGCC G[GA]^CG[CT]C AhdI 826 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 940 GGATC GGATC(4/5) AocI 908 CCTCAGG CC^T[ACGT]AGG AspEI 826 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 250 GGCGCC GGCGC^C BfaI 1024 CTAG C^TAG BglII 685 AGATCT A^GATCT BlpI 180 GCTGAGC GC^T[ACGT]AGC BpmI 642 CTCCAG CTGGAG(16/14) Bpu1102I 180 GCTGAGC GC^T[ACGT]AGC BsaHI 250 GGCGCC G[GA]^CG[CT]C BsaWI 937 TCCGGA [AT]^CCGG[AT] BseAI 937 TCCGGA T^CCGGA Bsp1407I 134 TGTACA T^GTACA BspEI 937 TCCGGA T^CCGGA BspLU11I 532 ACATGT A^CATGT BspMI 670 ACCTGC ACCTGC(4/8) BsrDI 419 GCAATG GCAATG(2/0) BsrGI 134 TGTACA T^GTACA BssSI 189 CTCGTG CACGAG(-5/-1) Bsu36I 908 CCTCAGG CC^T[ACGT]AGG CelII 180 GCTGAGC GC^T[ACGT]AGC DraII 128 AGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 186 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 826 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 908 CCTCAGG CC^T[ACGT]AGG EcoNI 657 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 128 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 739 GATATC GAT^ATC EcoT14I 480 CCAAGG C^C[AT][AT]GG HaeII 250 GGCGCC [GA]GCGC^[CT] HgaI 347 GCGTC GACGC(5/10) Hin1I 250 GGCGCC G[GA]^CG[CT]C HinfI 632 GAGTC G^A[ACGT]TC KasI 250 GGCGCC G^GCGCC MaeI 1024 CTAG C^TAG MroI 937 TCCGGA T^CCGGA MslI 531 CACATGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 176 GCCGGC GCC^GGC NarI 250 GGCGCC GG^CGCC NgoMIV 176 GCCGGC G^CCGGC NspI 532 ACATGT [GA]CATG^[CT] PflMI 57 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 632 GAGTC GAGTC(4/5) PpuMI 128 AGGTCCT [GA]G^G[AT]CC[CT] PstI 165 CTGCAG CTGCA^G SfaNI 263 GCATC GCATC(5/9) SfoI 250 GGCGCC GGC^GCC SspBI 134 TGTACA T^GTACA StyI 480 CCAAGG C^C[AT][AT]GG Van91I 57 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1023 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 7 CGCG CG^CG 9 CGCG AfaI 135 GTAC GT^AC 477 GTAC AflIII 280 ACGTGT A^C[GA][CT]GT 532 ACATGT Alw44I 428 GTGCAC G^TGCAC 747 GTGCAC ApaLI 428 GTGCAC G^TGCAC 747 GTGCAC BanI 14 GGCACC G^G[CT][GA]CC 250 GGCGCC BbsI 961 GAAGAC GAAGAC(2/6) 988 GAAGAC BcnI 22 CCGGG CC^[GC]GG 357 CCCGG BglI 21 GCCGGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 674 GCCTGCTGGGC BpuAI 961 GAAGAC GAAGAC(2/6) 988 GAAGAC BsiEI 117 CGGCCG CG[GA][CT]^CG 174 CGGCCG BstUI 7 CGCG CG^CG 9 CGCG BstYI 685 AGATCT [GA]^GATC[CT] 940 GGATCT DpnII 686 GATC ^GATC 941 GATC EaeI 117 CGGCCG [CT]^GGCC[GA] 174 CGGCCG EagI 117 CGGCCG C^GGCCG 174 CGGCCG EarI 223 CTCTTC CTCTTC(1/4) 973 GAAGAG EclXI 117 CGGCCG C^GGCCG 174 CGGCCG Eco52I 117 CGGCCG C^GGCCG 174 CGGCCG FauI 341 CCCGC CCCGC(4/6) 395 GCGGG HphI 374 TCACC GGTGA(8/7) 635 TCACC Ksp632I 223 CTCTTC CTCTTC(1/4) 973 GAAGAG MboI 686 GATC ^GATC 941 GATC MflI 685 AGATCT [GA]^GATC[CT] 940 GGATCT MspA1I 120 CCGCTG C[AC]G^C[GT]G 342 CCGCTG MvnI 7 CGCG CG^CG 9 CGCG NciI 22 CCGGG CC^[GC]GG 357 CCCGG NdeII 686 GATC ^GATC 941 GATC RsaI 135 GTAC GT^AC 477 GTAC Sau3AI 686 GATC ^GATC 941 GATC SfcI 165 CTGCAG C^T[GA][CT]AG 999 CTACAG XcmI 530 CCACATGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 744 CCAGTGCACTGGTGG XhoII 685 AGATCT [GA]^GATC[CT] 940 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AlwNI AspHI AvaII BbvI BmyI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MaeIII MboII MnlI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI Tsp45I TspRI