################################## # NO CUT ENZYMES ( 204 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeII MamI MflI MluI MroI MseI MslI MspI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I TseI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Asp700I 16 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 217 TGGCCA TGG^CCA BfaI 47 CTAG C^TAG BsaJI 257 CCTGGG C^C[ACGT][ACGT]GG BseRI 107 CTCCTC GAGGAG(10/8) BsmAI 105 GTCTC GTCTC(1/5) BsmFI 200 GGGAC GGGAC(10/14) BspDI 230 ATCGAT AT^CGAT BssKI 257 CCTGG ^CC[ACGT]GG BstNI 257 CCTGG CC^[AT]GG BstXI 303 CCAGCAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 230 ATCGAT AT^CGAT DdeI 101 CTGAG C^T[ACGT]AG EaeI 217 TGGCCA [CT]^GGCC[GA] EarI 34 GAAGAG CTCTTC(1/4) EcoRII 257 CCTGG ^CC[AT]GG FauI 273 CCCGC CCCGC(4/6) Fnu4HI 333 GCGGC GC^[ACGT]GC ItaI 333 GCGGC GC^[ACGT]GC Ksp632I 34 GAAGAG CTCTTC(1/4) MaeI 47 CTAG C^TAG MboII 34 GAAGA GAAGA(8/7) MfeI 113 CAATTG C^AATTG MluNI 217 TGGCCA TGG^CCA MscI 217 TGGCCA TGG^CCA MspA1I 331 CAGCGG C[AC]G^C[GT]G MunI 113 CAATTG C^AATTG MvaI 257 CCTGG CC^[AT]GG NlaIII 247 CATG CATG^ NlaIV 146 GGAACC GG[ACGT]^[ACGT]CC PflMI 236 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 103 GAGTC GAGTC(4/5) ScrFI 257 CCTGG CC^[ACGT]GG TaqI 231 TCGA T^CGA TfiI 53 GATTC G^A[AT]TC Tsp45I 224 GTGAC ^GT[GC]AC Tsp509I 114 AATT ^AATT TthHB8I 231 TCGA T^CGA Van91I 236 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 16 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AciI 274 CCGC CCGC(-3/-1) 333 GCGG AluI 18 AGCT AG^CT 138 AGCT AlwI 233 GATCC GGATC(4/5) 254 GATCC BsiYI 236 CCAATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 337 CCTCCTTACGG BslI 236 CCAATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 337 CCTCCTTACGG DpnII 233 GATC ^GATC 254 GATC HaeIII 218 GGCC GG^CC 335 GGCC HinfI 53 GATTC G^A[ACGT]TC 103 GAGTC MboI 233 GATC ^GATC 254 GATC NdeII 233 GATC ^GATC 254 GATC Sau3AI 233 GATC ^GATC 254 GATC TspRI 9 CACTG CA[GC]TG[ACGT][ACGT]^ 241 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MaeIII MnlI