Restriction map for
pFN21AE4438
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AlwNI AocI ApaI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BanII BbeI BbsI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeIII MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tsp45I
Tth111I XcmI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AhdI 676 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1112 AGCGCT AGC^GCT
AspEI 676 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 59 CCCGGG C^[CT]CG[GA]G
BalI 605 TGGCCA TGG^CCA
BcgI 422 CGAAGCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsoBI 59 CCCGGG C^[CT]CG[GA]G
BspDI 199 ATCGAT AT^CGAT
BssSI 111 CACGAG CACGAG(-5/-1)
CfoI 1113 GCGC GCG^C
ClaI 199 ATCGAT AT^CGAT
DraII 682 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 507 CCATGG C^C[GA][CT]GG
EaeI 605 TGGCCA [CT]^GGCC[GA]
Eam1105I 676 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1112 AGCGCT AGC^GCT
EcoNI 1131 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 682 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 507 CCATGG C^C[AT][AT]GG
FauI 406 CCCGC CCCGC(4/6)
HaeII 1112 AGCGCT [GA]GCGC^[CT]
HhaI 1113 GCGC GCG^C
HinP1I 1113 GCGC G^CGC
MluNI 605 TGGCCA TGG^CCA
MscI 605 TGGCCA TGG^CCA
MseI 760 TTAA T^TAA
NcoI 507 CCATGG C^CATGG
NspI 896 ACATGC [GA]CATG^[CT]
PflMI 41 CCATCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 946 GACTC GAGTC(4/5)
PpuMI 682 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1065 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI 870 CTACAG C^T[GA][CT]AG
SmaI 59 CCCGGG CCC^GGG
StyI 507 CCATGG C^C[AT][AT]GG
TaqI 200 TCGA T^CGA
TfiI 324 GATTC G^A[AT]TC
Tru9I 760 TTAA T^TAA
TthHB8I 200 TCGA T^CGA
Van91I 41 CCATCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 975 TCTAGA T^CTAGA
XmaCI 59 CCCGGG C^CCGGG
XmaI 59 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 846 GTAC GT^AC
1248 GTAC
Alw44I 439 GTGCAC G^TGCAC
909 GTGCAC
ApaLI 439 GTGCAC G^TGCAC
909 GTGCAC
Asp700I 588 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
861 GAAGGTATTC
BanI 714 GGTGCC G^G[CT][GA]CC
1093 GGCACC
BbrPI 385 CACGTG CAC^GTG
724 CACGTG
BfaI 7 CTAG C^TAG
976 CTAG
BpmI 1018 CTGGAG CTGGAG(16/14)
1354 CTGGAG
BsaAI 385 CACGTG [CT]AC^GT[GA]
724 CACGTG
BsgI 693 CTGCAC GTGCAG(16/14)
736 CTGCAC
BspMI 1058 ACCTGC ACCTGC(4/8)
315 GCAGGT
BstYI 176 AGATCC [GA]^GATC[CT]
617 GGATCT
EcoRI 655 GAATTC G^AATTC
1006 GAATTC
HinfI 324 GATTC G^A[ACGT]TC
946 GACTC
HphI 1236 GGTGA GGTGA(8/7)
215 TCACC
MaeI 7 CTAG C^TAG
976 CTAG
MflI 176 AGATCC [GA]^GATC[CT]
617 GGATCT
PmaCI 385 CACGTG CAC^GTG
724 CACGTG
PmlI 385 CACGTG CAC^GTG
724 CACGTG
RsaI 846 GTAC GT^AC
1248 GTAC
StuI 732 AGGCCT AGG^CCT
1029 AGGCCT
XhoII 176 AGATCC [GA]^GATC[CT]
617 GGATCT
XmnI 588 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
861 GAAGGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HpaII ItaI MaeII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PvuII Sau3AI Sau96I ScrFI
SfaNI TseI Tsp509I TspRI